Spermometer: electrical characterization of single boar sperm motility

    Research output: Contribution to journalArticleAcademicpeer-review

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    Abstract

    Objective: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. Design: Comparison of the optical data and electrical impedance data. Setting: Research laboratory at a university. Animal(s): Boar semen sample were used. Intervention(s): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. Main Outcome Measure(s): The sperm beat frequency and amplitude determined from the electrical impedance and video data. Result(s): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. Conclusion(s): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications. (C) 2016 by American Society for Reproductive Medicine.
    Original languageEnglish
    Pages (from-to)773-780
    JournalFertility and sterility
    Volume106
    Issue number3
    DOIs
    Publication statusPublished - 1 Sep 2016

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    Sperm Motility
    Spermatozoa
    Electric Impedance
    Microfluidics
    Temperature
    Intracytoplasmic Sperm Injections
    Caffeine
    Semen
    Outcome Assessment (Health Care)
    Research

    Keywords

    • EWI-27561
    • METIS-320932
    • IR-103136

    Cite this

    @article{60f45e148aa048c49f45f85a3dff869f,
    title = "Spermometer: electrical characterization of single boar sperm motility",
    abstract = "Objective: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. Design: Comparison of the optical data and electrical impedance data. Setting: Research laboratory at a university. Animal(s): Boar semen sample were used. Intervention(s): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. Main Outcome Measure(s): The sperm beat frequency and amplitude determined from the electrical impedance and video data. Result(s): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. Conclusion(s): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications. (C) 2016 by American Society for Reproductive Medicine.",
    keywords = "EWI-27561, METIS-320932, IR-103136",
    author = "{de Wagenaar}, B. and Geijs, {Daan J.} and {de Boer}, {Hans L.} and Bomer, {Johan G.} and Wouter Olthuis and {van den Berg}, Albert and Segerink, {Loes Irene}",
    note = "eemcs-eprint-27561",
    year = "2016",
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    day = "1",
    doi = "10.1016/j.fertnstert.2016.05.008",
    language = "English",
    volume = "106",
    pages = "773--780",
    journal = "Fertility and sterility",
    issn = "0015-0282",
    publisher = "Elsevier",
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    }

    Spermometer: electrical characterization of single boar sperm motility. / de Wagenaar, B.; Geijs, Daan J.; de Boer, Hans L.; Bomer, Johan G.; Olthuis, Wouter; van den Berg, Albert; Segerink, Loes Irene.

    In: Fertility and sterility, Vol. 106, No. 3, 01.09.2016, p. 773-780.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Spermometer: electrical characterization of single boar sperm motility

    AU - de Wagenaar, B.

    AU - Geijs, Daan J.

    AU - de Boer, Hans L.

    AU - Bomer, Johan G.

    AU - Olthuis, Wouter

    AU - van den Berg, Albert

    AU - Segerink, Loes Irene

    N1 - eemcs-eprint-27561

    PY - 2016/9/1

    Y1 - 2016/9/1

    N2 - Objective: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. Design: Comparison of the optical data and electrical impedance data. Setting: Research laboratory at a university. Animal(s): Boar semen sample were used. Intervention(s): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. Main Outcome Measure(s): The sperm beat frequency and amplitude determined from the electrical impedance and video data. Result(s): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. Conclusion(s): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications. (C) 2016 by American Society for Reproductive Medicine.

    AB - Objective: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. Design: Comparison of the optical data and electrical impedance data. Setting: Research laboratory at a university. Animal(s): Boar semen sample were used. Intervention(s): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. Main Outcome Measure(s): The sperm beat frequency and amplitude determined from the electrical impedance and video data. Result(s): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. Conclusion(s): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications. (C) 2016 by American Society for Reproductive Medicine.

    KW - EWI-27561

    KW - METIS-320932

    KW - IR-103136

    U2 - 10.1016/j.fertnstert.2016.05.008

    DO - 10.1016/j.fertnstert.2016.05.008

    M3 - Article

    VL - 106

    SP - 773

    EP - 780

    JO - Fertility and sterility

    JF - Fertility and sterility

    SN - 0015-0282

    IS - 3

    ER -