Spermometer: electrical characterization of single boar sperm motility

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)
1 Downloads (Pure)

Abstract

Objective: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. Design: Comparison of the optical data and electrical impedance data. Setting: Research laboratory at a university. Animal(s): Boar semen sample were used. Intervention(s): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. Main Outcome Measure(s): The sperm beat frequency and amplitude determined from the electrical impedance and video data. Result(s): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. Conclusion(s): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications. (C) 2016 by American Society for Reproductive Medicine.
Original languageEnglish
Pages (from-to)773-780
JournalFertility and sterility
Volume106
Issue number3
DOIs
Publication statusPublished - 1 Sep 2016

Fingerprint

Sperm Motility
Spermatozoa
Electric Impedance
Microfluidics
Temperature
Intracytoplasmic Sperm Injections
Caffeine
Semen
Outcome Assessment (Health Care)
Research

Keywords

  • EWI-27561
  • METIS-320932
  • IR-103136

Cite this

@article{60f45e148aa048c49f45f85a3dff869f,
title = "Spermometer: electrical characterization of single boar sperm motility",
abstract = "Objective: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. Design: Comparison of the optical data and electrical impedance data. Setting: Research laboratory at a university. Animal(s): Boar semen sample were used. Intervention(s): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. Main Outcome Measure(s): The sperm beat frequency and amplitude determined from the electrical impedance and video data. Result(s): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. Conclusion(s): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications. (C) 2016 by American Society for Reproductive Medicine.",
keywords = "EWI-27561, METIS-320932, IR-103136",
author = "{de Wagenaar}, B. and Geijs, {Daan J.} and {de Boer}, {Hans L.} and Bomer, {Johan G.} and Wouter Olthuis and {van den Berg}, Albert and Segerink, {Loes Irene}",
note = "eemcs-eprint-27561",
year = "2016",
month = "9",
day = "1",
doi = "10.1016/j.fertnstert.2016.05.008",
language = "English",
volume = "106",
pages = "773--780",
journal = "Fertility and sterility",
issn = "0015-0282",
publisher = "Elsevier",
number = "3",

}

Spermometer: electrical characterization of single boar sperm motility. / de Wagenaar, B.; Geijs, Daan J.; de Boer, Hans L.; Bomer, Johan G.; Olthuis, Wouter; van den Berg, Albert; Segerink, Loes Irene.

In: Fertility and sterility, Vol. 106, No. 3, 01.09.2016, p. 773-780.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Spermometer: electrical characterization of single boar sperm motility

AU - de Wagenaar, B.

AU - Geijs, Daan J.

AU - de Boer, Hans L.

AU - Bomer, Johan G.

AU - Olthuis, Wouter

AU - van den Berg, Albert

AU - Segerink, Loes Irene

N1 - eemcs-eprint-27561

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Objective: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. Design: Comparison of the optical data and electrical impedance data. Setting: Research laboratory at a university. Animal(s): Boar semen sample were used. Intervention(s): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. Main Outcome Measure(s): The sperm beat frequency and amplitude determined from the electrical impedance and video data. Result(s): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. Conclusion(s): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications. (C) 2016 by American Society for Reproductive Medicine.

AB - Objective: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. Design: Comparison of the optical data and electrical impedance data. Setting: Research laboratory at a university. Animal(s): Boar semen sample were used. Intervention(s): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. Main Outcome Measure(s): The sperm beat frequency and amplitude determined from the electrical impedance and video data. Result(s): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. Conclusion(s): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications. (C) 2016 by American Society for Reproductive Medicine.

KW - EWI-27561

KW - METIS-320932

KW - IR-103136

U2 - 10.1016/j.fertnstert.2016.05.008

DO - 10.1016/j.fertnstert.2016.05.008

M3 - Article

VL - 106

SP - 773

EP - 780

JO - Fertility and sterility

JF - Fertility and sterility

SN - 0015-0282

IS - 3

ER -