Spheroid culture as a tool for creating 3D complex tissues

E.M. Fennema, N.C. Rivron, Jeroen Rouwkema, Clemens van Blitterswijk, Jan de Boer

Research output: Contribution to journalArticleAcademicpeer-review

361 Citations (Scopus)

Abstract

3D cell culture methods confer a high degree of clinical and biological relevance to in vitro models. This is specifically the case with the spheroid culture, where a small aggregate of cells grows free of foreign materials. In spheroid cultures, cells secrete the extracellular matrix (ECM) in which they reside, and they can interact with cells from their original microenvironment. The value of spheroid cultures is increasing quickly due to novel microfabricated platforms amenable to high-throughput screening (HTS) and advances in cell culture. Here, we review new possibilities that combine the strengths of spheroid culture with new microenvironment fabrication methods that allow for the creation of large numbers of highly reproducible, complex tissues.
Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalTrends in biotechnology
Volume31
Issue number2
DOIs
Publication statusPublished - 2013

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Cell culture
Cell Culture Techniques
Tissue
Extracellular Matrix
Screening
Throughput
Fabrication

Keywords

  • IR-83313
  • METIS-293634

Cite this

Fennema, E. M., Rivron, N. C., Rouwkema, J., van Blitterswijk, C., & de Boer, J. (2013). Spheroid culture as a tool for creating 3D complex tissues. Trends in biotechnology, 31(2), 108-115. https://doi.org/10.1016/j.tibtech.2012.12.003
Fennema, E.M. ; Rivron, N.C. ; Rouwkema, Jeroen ; van Blitterswijk, Clemens ; de Boer, Jan. / Spheroid culture as a tool for creating 3D complex tissues. In: Trends in biotechnology. 2013 ; Vol. 31, No. 2. pp. 108-115.
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Fennema, EM, Rivron, NC, Rouwkema, J, van Blitterswijk, C & de Boer, J 2013, 'Spheroid culture as a tool for creating 3D complex tissues' Trends in biotechnology, vol. 31, no. 2, pp. 108-115. https://doi.org/10.1016/j.tibtech.2012.12.003

Spheroid culture as a tool for creating 3D complex tissues. / Fennema, E.M.; Rivron, N.C.; Rouwkema, Jeroen; van Blitterswijk, Clemens; de Boer, Jan.

In: Trends in biotechnology, Vol. 31, No. 2, 2013, p. 108-115.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Spheroid culture as a tool for creating 3D complex tissues

AU - Fennema, E.M.

AU - Rivron, N.C.

AU - Rouwkema, Jeroen

AU - van Blitterswijk, Clemens

AU - de Boer, Jan

PY - 2013

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AB - 3D cell culture methods confer a high degree of clinical and biological relevance to in vitro models. This is specifically the case with the spheroid culture, where a small aggregate of cells grows free of foreign materials. In spheroid cultures, cells secrete the extracellular matrix (ECM) in which they reside, and they can interact with cells from their original microenvironment. The value of spheroid cultures is increasing quickly due to novel microfabricated platforms amenable to high-throughput screening (HTS) and advances in cell culture. Here, we review new possibilities that combine the strengths of spheroid culture with new microenvironment fabrication methods that allow for the creation of large numbers of highly reproducible, complex tissues.

KW - IR-83313

KW - METIS-293634

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