Stamped Petri dishes for 3D cell culture and drug testing

Adithya Sridhar; Hans L. de Boer; Albert van den Berg; Severine le Gac

Stamped Petri dishes for 3D cell culture and drug testing

Adithya Sridhar, Hans L. de Boer, Albert van den Berg, Severine le Gac

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Downloads (Pure)

Abstract

We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).

Original language	Undefined
Title of host publication	2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013
Place of Publication	Californie
Publisher	Library of Congress
Pages	132-133
Number of pages	2
ISBN (Print)	978-0-9743611-8-5
Publication status	Published - 10 Apr 2013
Event	7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013 - Marina del Rey, United States Duration: 10 Apr 2013 → 12 Apr 2013 Conference number: 7

Publication series

Name	MMB
Publisher	Library of Congress
ISSN (Print)	1947-5160

Conference

Conference	7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013
Abbreviated title	MMB
Country/Territory	United States
City	Marina del Rey
Period	10/04/13 → 12/04/13

Keywords

EWI-24106
IR-88250
METIS-300223

Access to Document

http://eprints.eemcs.utwente.nl/secure2/24106/01/117.pdf

Cite this

@inproceedings{6dc783a38c914fae9b1aafed976694c1,

title = "Stamped Petri dishes for 3D cell culture and drug testing",

abstract = "We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).",

keywords = "EWI-24106, IR-88250, METIS-300223",

author = "Adithya Sridhar and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",

note = "eemcs-eprint-24106 ; 7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013 ; Conference date: 10-04-2013 Through 12-04-2013",

year = "2013",

month = apr,

day = "10",

language = "Undefined",

isbn = "978-0-9743611-8-5",

series = "MMB",

publisher = "Library of Congress",

pages = "132--133",

booktitle = "2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013",

}

Sridhar, A, de Boer, HL, van den Berg, A & le Gac, S 2013, Stamped Petri dishes for 3D cell culture and drug testing. in 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. MMB, Library of Congress, Californie, pp. 132-133, 7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013, Marina del Rey, California, United States, 10/04/13. <http://eprints.eemcs.utwente.nl/secure2/24106/01/117.pdf>

Stamped Petri dishes for 3D cell culture and drug testing. / Sridhar, Adithya; de Boer, Hans L.; van den Berg, Albert et al.
2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. Californie: Library of Congress, 2013. p. 132-133 (MMB).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Stamped Petri dishes for 3D cell culture and drug testing

AU - Sridhar, Adithya

AU - de Boer, Hans L.

AU - van den Berg, Albert

AU - le Gac, Severine

N1 - Conference code: 7

PY - 2013/4/10

Y1 - 2013/4/10

N2 - We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).

AB - We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).

KW - EWI-24106

KW - IR-88250

KW - METIS-300223

M3 - Conference contribution

SN - 978-0-9743611-8-5

T3 - MMB

SP - 132

EP - 133

BT - 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013

PB - Library of Congress

CY - Californie

T2 - 7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013

Y2 - 10 April 2013 through 12 April 2013

ER -