Stamped Petri dishes for 3D cell culture and drug testing

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).
Original languageUndefined
Title of host publication2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013
Place of PublicationCalifornie
PublisherLibrary of Congress
Pages132-133
Number of pages2
ISBN (Print)978-0-9743611-8-5
Publication statusPublished - 10 Apr 2013
Event7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013 - Marina del Rey, United States
Duration: 10 Apr 201312 Apr 2013
Conference number: 7

Publication series

NameMMB
PublisherLibrary of Congress
ISSN (Print)1947-5160

Conference

Conference7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013
Abbreviated titleMMB
CountryUnited States
CityMarina del Rey
Period10/04/1312/04/13

Keywords

  • EWI-24106
  • IR-88250
  • METIS-300223

Cite this

Sridhar, A., de Boer, H. L., van den Berg, A., & le Gac, S. (2013). Stamped Petri dishes for 3D cell culture and drug testing. In 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013 (pp. 132-133). (MMB). Californie: Library of Congress.
Sridhar, Adithya ; de Boer, Hans L. ; van den Berg, Albert ; le Gac, Severine. / Stamped Petri dishes for 3D cell culture and drug testing. 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. Californie : Library of Congress, 2013. pp. 132-133 (MMB).
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title = "Stamped Petri dishes for 3D cell culture and drug testing",
abstract = "We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).",
keywords = "EWI-24106, IR-88250, METIS-300223",
author = "Adithya Sridhar and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",
note = "eemcs-eprint-24106",
year = "2013",
month = "4",
day = "10",
language = "Undefined",
isbn = "978-0-9743611-8-5",
series = "MMB",
publisher = "Library of Congress",
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booktitle = "2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013",

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Sridhar, A, de Boer, HL, van den Berg, A & le Gac, S 2013, Stamped Petri dishes for 3D cell culture and drug testing. in 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. MMB, Library of Congress, Californie, pp. 132-133, 7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013, Marina del Rey, United States, 10/04/13.

Stamped Petri dishes for 3D cell culture and drug testing. / Sridhar, Adithya; de Boer, Hans L.; van den Berg, Albert; le Gac, Severine.

2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. Californie : Library of Congress, 2013. p. 132-133 (MMB).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Stamped Petri dishes for 3D cell culture and drug testing

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AU - de Boer, Hans L.

AU - van den Berg, Albert

AU - le Gac, Severine

N1 - eemcs-eprint-24106

PY - 2013/4/10

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N2 - We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).

AB - We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).

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KW - IR-88250

KW - METIS-300223

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SN - 978-0-9743611-8-5

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BT - 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013

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CY - Californie

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Sridhar A, de Boer HL, van den Berg A, le Gac S. Stamped Petri dishes for 3D cell culture and drug testing. In 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. Californie: Library of Congress. 2013. p. 132-133. (MMB).