Stamped Petri dishes for 3D cell culture and drug testing

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).
    Original languageUndefined
    Title of host publication2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013
    Place of PublicationCalifornie
    PublisherLibrary of Congress
    Pages132-133
    Number of pages2
    ISBN (Print)978-0-9743611-8-5
    Publication statusPublished - 10 Apr 2013
    Event7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013 - Marina del Rey, United States
    Duration: 10 Apr 201312 Apr 2013
    Conference number: 7

    Publication series

    NameMMB
    PublisherLibrary of Congress
    ISSN (Print)1947-5160

    Conference

    Conference7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013
    Abbreviated titleMMB
    CountryUnited States
    CityMarina del Rey
    Period10/04/1312/04/13

    Keywords

    • EWI-24106
    • IR-88250
    • METIS-300223

    Cite this

    Sridhar, A., de Boer, H. L., van den Berg, A., & le Gac, S. (2013). Stamped Petri dishes for 3D cell culture and drug testing. In 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013 (pp. 132-133). (MMB). Californie: Library of Congress.
    Sridhar, Adithya ; de Boer, Hans L. ; van den Berg, Albert ; le Gac, Severine. / Stamped Petri dishes for 3D cell culture and drug testing. 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. Californie : Library of Congress, 2013. pp. 132-133 (MMB).
    @inproceedings{6dc783a38c914fae9b1aafed976694c1,
    title = "Stamped Petri dishes for 3D cell culture and drug testing",
    abstract = "We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).",
    keywords = "EWI-24106, IR-88250, METIS-300223",
    author = "Adithya Sridhar and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",
    note = "eemcs-eprint-24106",
    year = "2013",
    month = "4",
    day = "10",
    language = "Undefined",
    isbn = "978-0-9743611-8-5",
    series = "MMB",
    publisher = "Library of Congress",
    pages = "132--133",
    booktitle = "2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013",

    }

    Sridhar, A, de Boer, HL, van den Berg, A & le Gac, S 2013, Stamped Petri dishes for 3D cell culture and drug testing. in 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. MMB, Library of Congress, Californie, pp. 132-133, 7th International Conference on Microtechnologies in Medicine and Biology, MMB 2013, Marina del Rey, United States, 10/04/13.

    Stamped Petri dishes for 3D cell culture and drug testing. / Sridhar, Adithya; de Boer, Hans L.; van den Berg, Albert; le Gac, Severine.

    2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. Californie : Library of Congress, 2013. p. 132-133 (MMB).

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - Stamped Petri dishes for 3D cell culture and drug testing

    AU - Sridhar, Adithya

    AU - de Boer, Hans L.

    AU - van den Berg, Albert

    AU - le Gac, Severine

    N1 - eemcs-eprint-24106

    PY - 2013/4/10

    Y1 - 2013/4/10

    N2 - We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).

    AB - We report here an experimentation platform directly made in a commercially available Petri dish for large-scale production of uniformly sized microtissues and their in situ characterization using both conventional microscopy and a scanning probe technique. The platform is realized by hot embossing microwell arrays in Petri dishes. Following suitable surface coating, microtissues spontaneously form in the microwells upon cell seeding, as shown for various cell lines (C2C12, MCF-7, HepG2 and HeLa). Finally, the microtissues are exposed to a model drug (ethanol), and they are imaged using fluorescence techniques and non-invasive scanning electrochemical microscopy (SECM).

    KW - EWI-24106

    KW - IR-88250

    KW - METIS-300223

    M3 - Conference contribution

    SN - 978-0-9743611-8-5

    T3 - MMB

    SP - 132

    EP - 133

    BT - 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013

    PB - Library of Congress

    CY - Californie

    ER -

    Sridhar A, de Boer HL, van den Berg A, le Gac S. Stamped Petri dishes for 3D cell culture and drug testing. In 2013 International Conference on Microtechnologies in Medicine and Biology, MMB 2013. Californie: Library of Congress. 2013. p. 132-133. (MMB).