Standardization of heparins by means of high performance liquid chromatography equipped with a low angle laser light scattering detector

W.E. Hennink, J.W.A. van den Berg, Jan Feijen

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Abstract

This study shows that HPLC-LALLS (high performance liquid chromatography with a light-scattering detector) is a convenient and reliable method for the characterization of standard heparin samples, provided that polyelectrolyte artefacts are suppressed by a suitable dialysis procedure. The method has been succesfully applied to the assessment of calibrated relations like the Mark-Houwink relation and the calibration curve of gel permeation chromatography. Furthermore, this study confirms that gel permeation chromatography for heparins cannot be calibrated with dextranes or polyethyleneglycols, but in addition to this well known fact it is shown that even the universal (hydrodynamic volume) calibration procedure does not work. Moreover, in addition to the familiar conclusion that heparins should be calibrated with heparins, it is shown that calibrated relations are valid only when the calibration has been made with heparin samples of the same origin (i.e. same tissue source, same animal source, and same purification scheme).
Original languageUndefined
Pages (from-to)463-475
JournalThrombosis Research
Volume45
Issue number5
DOIs
Publication statusPublished - 1987

Keywords

  • IR-69885

Cite this

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title = "Standardization of heparins by means of high performance liquid chromatography equipped with a low angle laser light scattering detector",
abstract = "This study shows that HPLC-LALLS (high performance liquid chromatography with a light-scattering detector) is a convenient and reliable method for the characterization of standard heparin samples, provided that polyelectrolyte artefacts are suppressed by a suitable dialysis procedure. The method has been succesfully applied to the assessment of calibrated relations like the Mark-Houwink relation and the calibration curve of gel permeation chromatography. Furthermore, this study confirms that gel permeation chromatography for heparins cannot be calibrated with dextranes or polyethyleneglycols, but in addition to this well known fact it is shown that even the universal (hydrodynamic volume) calibration procedure does not work. Moreover, in addition to the familiar conclusion that heparins should be calibrated with heparins, it is shown that calibrated relations are valid only when the calibration has been made with heparin samples of the same origin (i.e. same tissue source, same animal source, and same purification scheme).",
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journal = "Thrombosis Research",
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Standardization of heparins by means of high performance liquid chromatography equipped with a low angle laser light scattering detector. / Hennink, W.E.; van den Berg, J.W.A.; Feijen, Jan.

In: Thrombosis Research, Vol. 45, No. 5, 1987, p. 463-475.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Standardization of heparins by means of high performance liquid chromatography equipped with a low angle laser light scattering detector

AU - Hennink, W.E.

AU - van den Berg, J.W.A.

AU - Feijen, Jan

PY - 1987

Y1 - 1987

N2 - This study shows that HPLC-LALLS (high performance liquid chromatography with a light-scattering detector) is a convenient and reliable method for the characterization of standard heparin samples, provided that polyelectrolyte artefacts are suppressed by a suitable dialysis procedure. The method has been succesfully applied to the assessment of calibrated relations like the Mark-Houwink relation and the calibration curve of gel permeation chromatography. Furthermore, this study confirms that gel permeation chromatography for heparins cannot be calibrated with dextranes or polyethyleneglycols, but in addition to this well known fact it is shown that even the universal (hydrodynamic volume) calibration procedure does not work. Moreover, in addition to the familiar conclusion that heparins should be calibrated with heparins, it is shown that calibrated relations are valid only when the calibration has been made with heparin samples of the same origin (i.e. same tissue source, same animal source, and same purification scheme).

AB - This study shows that HPLC-LALLS (high performance liquid chromatography with a light-scattering detector) is a convenient and reliable method for the characterization of standard heparin samples, provided that polyelectrolyte artefacts are suppressed by a suitable dialysis procedure. The method has been succesfully applied to the assessment of calibrated relations like the Mark-Houwink relation and the calibration curve of gel permeation chromatography. Furthermore, this study confirms that gel permeation chromatography for heparins cannot be calibrated with dextranes or polyethyleneglycols, but in addition to this well known fact it is shown that even the universal (hydrodynamic volume) calibration procedure does not work. Moreover, in addition to the familiar conclusion that heparins should be calibrated with heparins, it is shown that calibrated relations are valid only when the calibration has been made with heparin samples of the same origin (i.e. same tissue source, same animal source, and same purification scheme).

KW - IR-69885

U2 - 10.1016/0049-3848(87)90309-4

DO - 10.1016/0049-3848(87)90309-4

M3 - Article

VL - 45

SP - 463

EP - 475

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

IS - 5

ER -