Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips

Anke R. Vollertsen, Stefan Dekker, Britt A.M. Wesselink, Rob Haverkate, Johan G. Bomer, Hoon Suk Rho, Robert Jan Boom, Maciej Skolimowski, Marko Blom, Andries van der Meer, Robert Passier, Albert van den Berg, Mathieu Odijk

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).
Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publicationMicroTAS
Pages2199-2201
Number of pages3
Publication statusPublished - 11 Nov 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Province of China
Duration: 10 Nov 201815 Nov 2018
Conference number: 22
https://cbmsociety.org/conferences/microtas2018/

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated titleMicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period10/11/1815/11/18
Internet address

Fingerprint

Microfluidics
Lab-On-A-Chip Devices
Human Umbilical Vein Endothelial Cells
Drug Industry
Cell Culture Techniques
Pressure
Equipment and Supplies

Keywords

  • Parallelized
  • Standardization
  • Microfluidic large-scale integration
  • Cell culture

Cite this

Vollertsen, A. R., Dekker, S., Wesselink, B. A. M., Haverkate, R., Bomer, J. G., Rho, H. S., ... Odijk, M. (2018). Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences: MicroTAS (pp. 2199-2201)
Vollertsen, Anke R. ; Dekker, Stefan ; Wesselink, Britt A.M. ; Haverkate, Rob ; Bomer, Johan G. ; Rho, Hoon Suk ; Boom, Robert Jan ; Skolimowski, Maciej ; Blom, Marko ; van der Meer, Andries ; Passier, Robert ; van den Berg, Albert ; Odijk, Mathieu . / Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences: MicroTAS. 2018. pp. 2199-2201
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title = "Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips",
abstract = "Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).",
keywords = "Parallelized, Standardization, Microfluidic large-scale integration, Cell culture",
author = "Vollertsen, {Anke R.} and Stefan Dekker and Wesselink, {Britt A.M.} and Rob Haverkate and Bomer, {Johan G.} and Rho, {Hoon Suk} and Boom, {Robert Jan} and Maciej Skolimowski and Marko Blom and {van der Meer}, Andries and Robert Passier and {van den Berg}, Albert and Mathieu Odijk",
year = "2018",
month = "11",
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language = "English",
isbn = "978-0-578-40530-8",
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booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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Vollertsen, AR, Dekker, S, Wesselink, BAM, Haverkate, R, Bomer, JG, Rho, HS, Boom, RJ, Skolimowski, M, Blom, M, van der Meer, A, Passier, R, van den Berg, A & Odijk, M 2018, Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences: MicroTAS. pp. 2199-2201, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, Kaohsiung, Taiwan, Province of China, 10/11/18.

Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. / Vollertsen, Anke R.; Dekker, Stefan ; Wesselink, Britt A.M.; Haverkate, Rob ; Bomer, Johan G.; Rho, Hoon Suk; Boom, Robert Jan; Skolimowski, Maciej; Blom, Marko; van der Meer, Andries; Passier, Robert; van den Berg, Albert ; Odijk, Mathieu .

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences: MicroTAS. 2018. p. 2199-2201.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips

AU - Vollertsen, Anke R.

AU - Dekker, Stefan

AU - Wesselink, Britt A.M.

AU - Haverkate, Rob

AU - Bomer, Johan G.

AU - Rho, Hoon Suk

AU - Boom, Robert Jan

AU - Skolimowski, Maciej

AU - Blom, Marko

AU - van der Meer, Andries

AU - Passier, Robert

AU - van den Berg, Albert

AU - Odijk, Mathieu

PY - 2018/11/11

Y1 - 2018/11/11

N2 - Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).

AB - Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).

KW - Parallelized

KW - Standardization

KW - Microfluidic large-scale integration

KW - Cell culture

M3 - Conference contribution

SN - 978-0-578-40530-8

SP - 2199

EP - 2201

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences

ER -

Vollertsen AR, Dekker S, Wesselink BAM, Haverkate R, Bomer JG, Rho HS et al. Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences: MicroTAS. 2018. p. 2199-2201