Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips

Anke R. Vollertsen, Stefan Dekker, Britt A.M. Wesselink, Rob Haverkate, Johan G. Bomer, Hoon Suk Rho, Robert Jan Boom, Maciej Skolimowski, Marko Blom, Andries van der Meer, Robert Passier, Albert van den Berg, Mathieu Odijk

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Abstract

Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).
Original languageEnglish
Title of host publicationMicroTAS 2018
Subtitle of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsFan-Gang Tseng, Gwo-Bin Lee
PublisherThe Chemical and Biological Microsystems Society
Pages2199-2201
Number of pages3
ISBN (Electronic)978-1-510-89757-1
ISBN (Print)978-0-578-40530-8
Publication statusPublished - 11 Nov 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Province of China
Duration: 10 Nov 201815 Nov 2018
Conference number: 22
https://cbmsociety.org/conferences/microtas2018/

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated titleMicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period10/11/1815/11/18
Internet address

Keywords

  • Parallelized
  • Standardization
  • Microfluidic large-scale integration
  • Cell culture

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  • Cite this

    Vollertsen, A. R., Dekker, S., Wesselink, B. A. M., Haverkate, R., Bomer, J. G., Rho, H. S., ... Odijk, M. (2018). Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. In F-G. Tseng, & G-B. Lee (Eds.), MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 2199-2201). The Chemical and Biological Microsystems Society.