Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips

Anke R. Vollertsen; Stefan  Dekker; Britt A.M. Wesselink; Rob  Haverkate; Johan G. Bomer; Hoon Suk Rho; Robert Jan Boom; Maciej Skolimowski; Marko Blom; Andries van der Meer; Robert Passier; Albert  van den Berg; Mathieu  Odijk

Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips

Anke R. Vollertsen, Stefan Dekker, Britt A.M. Wesselink, Rob Haverkate, Johan G. Bomer, Hoon Suk Rho, Robert Jan Boom, Maciej Skolimowski, Marko Blom, Andries van der Meer, Robert Passier, Albert van den Berg, Mathieu Odijk

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

2 Citations (Scopus)

274 Downloads (Pure)

Abstract

Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).

Original language	English
Title of host publication	MicroTAS 2018
Subtitle of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	Fan-Gang Tseng, Gwo-Bin Lee
Publisher	The Chemical and Biological Microsystems Society
Pages	2199-2201
Number of pages	3
ISBN (Electronic)	978-1-510-89757-1
ISBN (Print)	978-0-578-40530-8
Publication status	Published - 11 Nov 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan Duration: 10 Nov 2018 → 15 Nov 2018 Conference number: 22 https://cbmsociety.org/conferences/microtas2018/

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated title	MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	10/11/18 → 15/11/18
Internet address	https://cbmsociety.org/conferences/microtas2018/

Keywords

2019 OA procedure
Standardization
Microfluidic large-scale integration
Cell culture
Parallelized

Access to Document

ArticleFinal published version, 1.08 MBLicence: Taverne

CHEMINAS Poster Award
Vollertsen, A. R. (Recipient), 15 Nov 2018
Prize

File

Poster Prize at MicroTAS Conference
Vollertsen, A. R. & Dekker, S.
19/11/18
2 items of Media coverage
Press/Media: Other

Cite this

Vollertsen, A. R., Dekker, S., Wesselink, B. A. M., Haverkate, R., Bomer, J. G., Rho, H. S., Boom, R. J., Skolimowski, M., Blom, M., van der Meer, A., Passier, R., van den Berg, A., & Odijk, M. (2018). Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. In F.-G. Tseng, & G.-B. Lee (Eds.), MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 2199-2201). The Chemical and Biological Microsystems Society.

Vollertsen, Anke R. ; Dekker, Stefan ; Wesselink, Britt A.M. et al. / Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / Fan-Gang Tseng ; Gwo-Bin Lee. The Chemical and Biological Microsystems Society, 2018. pp. 2199-2201

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title = "Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips",

abstract = "Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).",

keywords = "2019 OA procedure, Standardization, Microfluidic large-scale integration, Cell culture, Parallelized",

author = "Vollertsen, {Anke R.} and Stefan Dekker and Wesselink, {Britt A.M.} and Rob Haverkate and Bomer, {Johan G.} and Rho, {Hoon Suk} and Boom, {Robert Jan} and Maciej Skolimowski and Marko Blom and {van der Meer}, Andries and Robert Passier and {van den Berg}, Albert and Mathieu Odijk",

year = "2018",

month = nov,

day = "11",

language = "English",

isbn = "978-0-578-40530-8",

pages = "2199--2201",

editor = "Fan-Gang Tseng and Gwo-Bin Lee",

booktitle = "MicroTAS 2018",

publisher = "The Chemical and Biological Microsystems Society",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, MicroTAS 2018 ; Conference date: 10-11-2018 Through 15-11-2018",

url = "https://cbmsociety.org/conferences/microtas2018/",

}

Vollertsen, AR, Dekker, S, Wesselink, BAM, Haverkate, R, Bomer, JG, Rho, HS, Boom, RJ, Skolimowski, M, Blom, M, van der Meer, A , Passier, R , van den Berg, A & Odijk, M 2018, Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. in F-G Tseng & G-B Lee (eds), MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, pp. 2199-2201, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, Kaohsiung, Taiwan, 10/11/18.

Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. / Vollertsen, Anke R.; Dekker, Stefan ; Wesselink, Britt A.M. et al.
MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Fan-Gang Tseng; Gwo-Bin Lee. The Chemical and Biological Microsystems Society, 2018. p. 2199-2201.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips

AU - Vollertsen, Anke R.

AU - Dekker, Stefan

AU - Wesselink, Britt A.M.

AU - Haverkate, Rob

AU - Bomer, Johan G.

AU - Rho, Hoon Suk

AU - Boom, Robert Jan

AU - Skolimowski, Maciej

AU - Blom, Marko

AU - van der Meer, Andries

AU - Passier, Robert

AU - van den Berg, Albert

AU - Odijk, Mathieu

N1 - Conference code: 22

PY - 2018/11/11

Y1 - 2018/11/11

N2 - Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).

AB - Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).

KW - 2019 OA procedure

KW - Standardization

KW - Microfluidic large-scale integration

KW - Cell culture

KW - Parallelized

M3 - Conference contribution

SN - 978-0-578-40530-8

SP - 2199

EP - 2201

BT - MicroTAS 2018

A2 - Tseng, Fan-Gang

A2 - Lee, Gwo-Bin

PB - The Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018

Y2 - 10 November 2018 through 15 November 2018

ER -

Vollertsen AR, Dekker S, Wesselink BAM, Haverkate R, Bomer JG, Rho HS et al. Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips. In Tseng FG, Lee GB, editors, MicroTAS 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society. 2018. p. 2199-2201

Standardized, Modular Parallelization Platform for Microfluidic Large-Scale Integration Cell Culturing Chips

Abstract

Conference

Keywords

Access to Document

Fingerprint

Prizes

CHEMINAS Poster Award

Press/Media

Poster Prize at MicroTAS Conference

Cite this