State of the art of shear driven chromatography

Veronika Fekete, David Clicq, Wim de Malsche, Johannes G.E. Gardeniers, Gert Desmet

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17 Citations (Scopus)


The present paper reports on the experimental difficulties encountered when trying to realize the full potential of shear-driven chromatography in nanochannels. While it theoretically offers the possibility to yield over 10,000 plates per centimetre in a few seconds, the practical realization of this potential requires a detector miniaturisation that is carried to the extreme combined with very high sampling rates. In the present study, a charge coupled device camera and a photomultiplier tube combined with pinhole were tested as detector. Despite the fact that the photomultiplier tube could offer a higher sampling rate and a better sensitivity, the charge coupled device turned out to be better suited for the current set-up because of inevitable problems with the stray-light transported through the glass channel wall. The chemistry of the separation surface was additionally studied getting more homogenous coating, thus higher separation efficiency. Having also carried out a number of mechanical improvements, it is now possible to measure separations at a distance of 8 mm downstream from the injection point. This is four times further downstream than ever before while realizing a four components mixture separation in less than 1.5 s, with a plate generation velocity of about 2000–7000 plates per second depending on the sample.
Original languageUndefined
Pages (from-to)2-11
Number of pages10
JournalJournal of chromatography A
Issue number1
Publication statusPublished - 2007


  • IR-74709
  • METIS-244688

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