State of the art of shear driven chromatography

Veronika Fekete, David Clicq, Wim de Malsche, Johannes G.E. Gardeniers, Gert Desmet

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

The present paper reports on the experimental difficulties encountered when trying to realize the full potential of shear-driven chromatography in nanochannels. While it theoretically offers the possibility to yield over 10,000 plates per centimetre in a few seconds, the practical realization of this potential requires a detector miniaturisation that is carried to the extreme combined with very high sampling rates. In the present study, a charge coupled device camera and a photomultiplier tube combined with pinhole were tested as detector. Despite the fact that the photomultiplier tube could offer a higher sampling rate and a better sensitivity, the charge coupled device turned out to be better suited for the current set-up because of inevitable problems with the stray-light transported through the glass channel wall. The chemistry of the separation surface was additionally studied getting more homogenous coating, thus higher separation efficiency. Having also carried out a number of mechanical improvements, it is now possible to measure separations at a distance of 8 mm downstream from the injection point. This is four times further downstream than ever before while realizing a four components mixture separation in less than 1.5 s, with a plate generation velocity of about 2000–7000 plates per second depending on the sample.
Original languageUndefined
Pages (from-to)2-11
Number of pages10
JournalJournal of chromatography A
Volume1149
Issue number1
DOIs
Publication statusPublished - 2007

Keywords

  • IR-74709
  • METIS-244688

Cite this

Fekete, Veronika ; Clicq, David ; de Malsche, Wim ; Gardeniers, Johannes G.E. ; Desmet, Gert. / State of the art of shear driven chromatography. In: Journal of chromatography A. 2007 ; Vol. 1149, No. 1. pp. 2-11.
@article{0d800d50f6554a878773a5bd853c2435,
title = "State of the art of shear driven chromatography",
abstract = "The present paper reports on the experimental difficulties encountered when trying to realize the full potential of shear-driven chromatography in nanochannels. While it theoretically offers the possibility to yield over 10,000 plates per centimetre in a few seconds, the practical realization of this potential requires a detector miniaturisation that is carried to the extreme combined with very high sampling rates. In the present study, a charge coupled device camera and a photomultiplier tube combined with pinhole were tested as detector. Despite the fact that the photomultiplier tube could offer a higher sampling rate and a better sensitivity, the charge coupled device turned out to be better suited for the current set-up because of inevitable problems with the stray-light transported through the glass channel wall. The chemistry of the separation surface was additionally studied getting more homogenous coating, thus higher separation efficiency. Having also carried out a number of mechanical improvements, it is now possible to measure separations at a distance of 8 mm downstream from the injection point. This is four times further downstream than ever before while realizing a four components mixture separation in less than 1.5 s, with a plate generation velocity of about 2000–7000 plates per second depending on the sample.",
keywords = "IR-74709, METIS-244688",
author = "Veronika Fekete and David Clicq and {de Malsche}, Wim and Gardeniers, {Johannes G.E.} and Gert Desmet",
note = "26th International Symposium on Chromatography",
year = "2007",
doi = "10.1016/j.chroma.2007.01.120",
language = "Undefined",
volume = "1149",
pages = "2--11",
journal = "Journal of chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1",

}

State of the art of shear driven chromatography. / Fekete, Veronika; Clicq, David; de Malsche, Wim; Gardeniers, Johannes G.E.; Desmet, Gert.

In: Journal of chromatography A, Vol. 1149, No. 1, 2007, p. 2-11.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - State of the art of shear driven chromatography

AU - Fekete, Veronika

AU - Clicq, David

AU - de Malsche, Wim

AU - Gardeniers, Johannes G.E.

AU - Desmet, Gert

N1 - 26th International Symposium on Chromatography

PY - 2007

Y1 - 2007

N2 - The present paper reports on the experimental difficulties encountered when trying to realize the full potential of shear-driven chromatography in nanochannels. While it theoretically offers the possibility to yield over 10,000 plates per centimetre in a few seconds, the practical realization of this potential requires a detector miniaturisation that is carried to the extreme combined with very high sampling rates. In the present study, a charge coupled device camera and a photomultiplier tube combined with pinhole were tested as detector. Despite the fact that the photomultiplier tube could offer a higher sampling rate and a better sensitivity, the charge coupled device turned out to be better suited for the current set-up because of inevitable problems with the stray-light transported through the glass channel wall. The chemistry of the separation surface was additionally studied getting more homogenous coating, thus higher separation efficiency. Having also carried out a number of mechanical improvements, it is now possible to measure separations at a distance of 8 mm downstream from the injection point. This is four times further downstream than ever before while realizing a four components mixture separation in less than 1.5 s, with a plate generation velocity of about 2000–7000 plates per second depending on the sample.

AB - The present paper reports on the experimental difficulties encountered when trying to realize the full potential of shear-driven chromatography in nanochannels. While it theoretically offers the possibility to yield over 10,000 plates per centimetre in a few seconds, the practical realization of this potential requires a detector miniaturisation that is carried to the extreme combined with very high sampling rates. In the present study, a charge coupled device camera and a photomultiplier tube combined with pinhole were tested as detector. Despite the fact that the photomultiplier tube could offer a higher sampling rate and a better sensitivity, the charge coupled device turned out to be better suited for the current set-up because of inevitable problems with the stray-light transported through the glass channel wall. The chemistry of the separation surface was additionally studied getting more homogenous coating, thus higher separation efficiency. Having also carried out a number of mechanical improvements, it is now possible to measure separations at a distance of 8 mm downstream from the injection point. This is four times further downstream than ever before while realizing a four components mixture separation in less than 1.5 s, with a plate generation velocity of about 2000–7000 plates per second depending on the sample.

KW - IR-74709

KW - METIS-244688

U2 - 10.1016/j.chroma.2007.01.120

DO - 10.1016/j.chroma.2007.01.120

M3 - Article

VL - 1149

SP - 2

EP - 11

JO - Journal of chromatography A

JF - Journal of chromatography A

SN - 0021-9673

IS - 1

ER -