Stimulated-emission pumping enabling sub-diffraction-limited spatial resolution in coherent anti-Stokes Raman scattering microscopy

Carsten Cleff, Petra Groß, Carsten Fallnich, Herman L. Offerhaus, Jennifer L. Herek, Kai Kruse, Willem P. Beeker, Chris J. Lee, Klaus-Jochen Boller

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)
54 Downloads (Pure)

Abstract

We present a theoretical investigation of stimulated emission pumping to achieve sub-diffraction-limited spatial resolution in coherent anti-Stokes Raman scattering (CARS) microscopy. A pair of control light fields is used to prepopulate the Raman state involved in the CARS process prior to the CARS signal generation. Efficient prepopulation is achieved by employing a path via an electronic or vibrational state. Thereby, the buildup of a coherence between the ground and Raman state during irradiation by the light fields for CARS signal generation is prevented, resulting in the suppression of the CARS intensity by more than 99.8%. Two-dimensional spatial excitation profiles have been calculated using donut-shaped spatial profiles for the control light fields, thus, an intensity-dependent narrowing of the CARS excitation profiles below the diffraction limit. Using computer-generated test images we demonstrate a resolution beyond the diffraction limit for CARS microscopy, which is scalable by the control light field intensity, similar to stimulated emission depletion microscopy.
Original languageEnglish
Article number033830
Number of pages9
JournalPhysical review A: Atomic, molecular, and optical physics
Volume87
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

stimulated emission
pumping
spatial resolution
Raman spectra
microscopy
diffraction
profiles
vibrational states
excitation
depletion
retarding
irradiation
ground state
electronics

Cite this

@article{c32d479f4447476a8e879ecef6534c25,
title = "Stimulated-emission pumping enabling sub-diffraction-limited spatial resolution in coherent anti-Stokes Raman scattering microscopy",
abstract = "We present a theoretical investigation of stimulated emission pumping to achieve sub-diffraction-limited spatial resolution in coherent anti-Stokes Raman scattering (CARS) microscopy. A pair of control light fields is used to prepopulate the Raman state involved in the CARS process prior to the CARS signal generation. Efficient prepopulation is achieved by employing a path via an electronic or vibrational state. Thereby, the buildup of a coherence between the ground and Raman state during irradiation by the light fields for CARS signal generation is prevented, resulting in the suppression of the CARS intensity by more than 99.8{\%}. Two-dimensional spatial excitation profiles have been calculated using donut-shaped spatial profiles for the control light fields, thus, an intensity-dependent narrowing of the CARS excitation profiles below the diffraction limit. Using computer-generated test images we demonstrate a resolution beyond the diffraction limit for CARS microscopy, which is scalable by the control light field intensity, similar to stimulated emission depletion microscopy.",
author = "Carsten Cleff and Petra Gro{\ss} and Carsten Fallnich and Offerhaus, {Herman L.} and Herek, {Jennifer L.} and Kai Kruse and Beeker, {Willem P.} and Lee, {Chris J.} and Klaus-Jochen Boller",
year = "2013",
doi = "10.1103/PhysRevA.87.033830",
language = "English",
volume = "87",
journal = "Physical review A : atomic, molecular, and optical physics and quantum information",
issn = "2469-9926",
publisher = "American Physical Society",
number = "3",

}

TY - JOUR

T1 - Stimulated-emission pumping enabling sub-diffraction-limited spatial resolution in coherent anti-Stokes Raman scattering microscopy

AU - Cleff, Carsten

AU - Groß, Petra

AU - Fallnich, Carsten

AU - Offerhaus, Herman L.

AU - Herek, Jennifer L.

AU - Kruse, Kai

AU - Beeker, Willem P.

AU - Lee, Chris J.

AU - Boller, Klaus-Jochen

PY - 2013

Y1 - 2013

N2 - We present a theoretical investigation of stimulated emission pumping to achieve sub-diffraction-limited spatial resolution in coherent anti-Stokes Raman scattering (CARS) microscopy. A pair of control light fields is used to prepopulate the Raman state involved in the CARS process prior to the CARS signal generation. Efficient prepopulation is achieved by employing a path via an electronic or vibrational state. Thereby, the buildup of a coherence between the ground and Raman state during irradiation by the light fields for CARS signal generation is prevented, resulting in the suppression of the CARS intensity by more than 99.8%. Two-dimensional spatial excitation profiles have been calculated using donut-shaped spatial profiles for the control light fields, thus, an intensity-dependent narrowing of the CARS excitation profiles below the diffraction limit. Using computer-generated test images we demonstrate a resolution beyond the diffraction limit for CARS microscopy, which is scalable by the control light field intensity, similar to stimulated emission depletion microscopy.

AB - We present a theoretical investigation of stimulated emission pumping to achieve sub-diffraction-limited spatial resolution in coherent anti-Stokes Raman scattering (CARS) microscopy. A pair of control light fields is used to prepopulate the Raman state involved in the CARS process prior to the CARS signal generation. Efficient prepopulation is achieved by employing a path via an electronic or vibrational state. Thereby, the buildup of a coherence between the ground and Raman state during irradiation by the light fields for CARS signal generation is prevented, resulting in the suppression of the CARS intensity by more than 99.8%. Two-dimensional spatial excitation profiles have been calculated using donut-shaped spatial profiles for the control light fields, thus, an intensity-dependent narrowing of the CARS excitation profiles below the diffraction limit. Using computer-generated test images we demonstrate a resolution beyond the diffraction limit for CARS microscopy, which is scalable by the control light field intensity, similar to stimulated emission depletion microscopy.

U2 - 10.1103/PhysRevA.87.033830

DO - 10.1103/PhysRevA.87.033830

M3 - Article

VL - 87

JO - Physical review A : atomic, molecular, and optical physics and quantum information

JF - Physical review A : atomic, molecular, and optical physics and quantum information

SN - 2469-9926

IS - 3

M1 - 033830

ER -