Strong and Reversible Monovalent Supramolecular Protein Immobilization

Jacqui F. Young, Hoang D. Nguyen, Lanti Yang, Jurriaan Huskens, Pascal Jonkheijm, Luc Brunsveld

Research output: Contribution to journalArticleAcademic

72 Citations (Scopus)

Abstract

Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand.
Original languageUndefined
Pages (from-to)180-183
JournalChemBioChem
Volume11
Issue number2
DOIs
Publication statusPublished - 2010

Keywords

  • protein immobilization
  • IR-72762
  • host–guest systems
  • protein ligation
  • Supramolecular Chemistry
  • Cucurbituril

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