Strong and reversible monovalent supramolecular protein immobilization

Jacqui F. Young; Hoang D. Nguyen; Lanti Yang; Jurriaan Huskens; Pascal Jonkheijm; Luc Brunsveld

doi:10.1002/cbic.200900599

Strong and reversible monovalent supramolecular protein immobilization

Jacqui F. Young, Hoang D. Nguyen, Lanti Yang, Jurriaan Huskens, Pascal Jonkheijm, Luc Brunsveld

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand.

Original language	English
Pages (from-to)	180-183
Number of pages	4
Journal	ChemBioChem
Volume	11
Issue number	2
DOIs	https://doi.org/10.1002/cbic.200900599
Publication status	Published - 2010

Keywords

Protein immobilization
Host–guest systems
Protein ligation
Supramolecular chemistry
Cucurbituril

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10.1002/cbic.200900599

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abstract = "Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand.",

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AU - Young, Jacqui F.

AU - Nguyen, Hoang D.

AU - Yang, Lanti

AU - Huskens, Jurriaan

AU - Jonkheijm, Pascal

AU - Brunsveld, Luc

PY - 2010

Y1 - 2010

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AB - Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand.

KW - Protein immobilization

KW - Host–guest systems

KW - Protein ligation

KW - Supramolecular chemistry

KW - Cucurbituril

U2 - 10.1002/cbic.200900599

DO - 10.1002/cbic.200900599

M3 - Article

SN - 1439-4227

VL - 11

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JO - ChemBioChem

JF - ChemBioChem

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Strong and reversible monovalent supramolecular protein immobilization

Abstract

Keywords

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