Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake

Rindia M. Putri, Carolina Allende-Ballestero, Daniel Luque, Robin Klem, Katerina Asteria Rousou, Aijie Liu, Christoph H.H. Traulsen, W. Frederik Rurup, Melissa S.T. Koay, José R. Castón*, Jeroen J.L.M. Cornelissen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)
264 Downloads (Pure)

Abstract

Recent years have witnessed the emergence of bacterial semiorganelle encapsulins as promising platforms for bio-nanotechnology. To advance the development of encapsulins as nanoplatforms, a functional and structural basis of these assemblies is required. Encapsulin from Brevibacterium linens is known to be a protein-based vessel for an enzyme cargo in its cavity, which could be replaced with a foreign cargo, resulting in a modified encapsulin. Here, we characterize the native structure of B. linens encapsulins with both native and foreign cargo using cryo-electron microscopy (cryo-EM). Furthermore, by harnessing the confined enzyme (i.e., a peroxidase), we demonstrate the functionality of the encapsulin for an in vitro surface-immobilized catalysis in a cascade pathway with an additional enzyme, glucose oxidase. We also demonstrate the in vivo functionality of the encapsulin for cellular uptake using mammalian macrophages. Unraveling both the structure and functionality of the encapsulins allows transforming biological nanocompartments into functional systems.

Original languageEnglish
Pages (from-to)12796-12804
Number of pages9
JournalACS nano
Volume11
Issue number12
DOIs
Publication statusPublished - 26 Dec 2017

Keywords

  • bacterial compartments
  • cryo-electron microscopy
  • encapsulin
  • nanoplatforms
  • nanoreactors

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