DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.
|Number of pages||1|
|Publication status||Published - Sep 2014|
|Event||40th International Conference on Micro & Nano Engineering, MNE 2014 - Lausanne, Switzerland|
Duration: 22 Sep 2014 → 26 Sep 2014
Conference number: 40
|Conference||40th International Conference on Micro & Nano Engineering, MNE 2014|
|Period||22/09/14 → 26/09/14|
- Capillary electrophoresis
- femtosecond laser writing
- microfluidic chip
- IOMS-SNS: SENSORS
- DNA separation
Pollnau, M., Hammer, M., Dongre, C., & Hoekstra, H. (2014). Sub-base-pair resolution during DNA separation in an optofluidic chip. Page 26. Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.