Sub-base-pair resolution during DNA separation in an optofluidic chip

Markus Pollnau, Manfred Hammer, C. Dongre, Hugo Hoekstra

Abstract

DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.
Original languageUndefined
PagesPage 26
Number of pages1
StatePublished - Sep 2014
Event40th International Conference on Micro & Nano Engineering, MNE 2014 - Lausanne, Switzerland

Conference

Conference40th International Conference on Micro & Nano Engineering, MNE 2014
Abbreviated titleMNE
CountrySwitzerland
CityLausanne
Period22/09/1426/09/14

Fingerprint

Base Pairing
DNA Sequence Analysis
DNA

Keywords

  • Capillary electrophoresis
  • femtosecond laser writing
  • EWI-25107
  • microfluidic chip
  • IR-91972
  • IOMS-SNS: SENSORS
  • DNA separation

Cite this

Pollnau, M., Hammer, M., Dongre, C., & Hoekstra, H. (2014). Sub-base-pair resolution during DNA separation in an optofluidic chip. Page 26. Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.

Pollnau, Markus; Hammer, Manfred; Dongre, C.; Hoekstra, Hugo / Sub-base-pair resolution during DNA separation in an optofluidic chip.

2014. Page 26 Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.

Research output: ScientificPaper

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title = "Sub-base-pair resolution during DNA separation in an optofluidic chip",
abstract = "DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.",
keywords = "Capillary electrophoresis, femtosecond laser writing, EWI-25107, microfluidic chip, IR-91972, IOMS-SNS: SENSORS, DNA separation",
author = "Markus Pollnau and Manfred Hammer and C. Dongre and Hugo Hoekstra",
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Pollnau, M, Hammer, M, Dongre, C & Hoekstra, H 2014, 'Sub-base-pair resolution during DNA separation in an optofluidic chip' Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland, 22/09/14 - 26/09/14, pp. Page 26.

Sub-base-pair resolution during DNA separation in an optofluidic chip. / Pollnau, Markus; Hammer, Manfred; Dongre, C.; Hoekstra, Hugo.

2014. Page 26 Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.

Research output: ScientificPaper

TY - CONF

T1 - Sub-base-pair resolution during DNA separation in an optofluidic chip

AU - Pollnau,Markus

AU - Hammer,Manfred

AU - Dongre,C.

AU - Hoekstra,Hugo

PY - 2014/9

Y1 - 2014/9

N2 - DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.

AB - DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.

KW - Capillary electrophoresis

KW - femtosecond laser writing

KW - EWI-25107

KW - microfluidic chip

KW - IR-91972

KW - IOMS-SNS: SENSORS

KW - DNA separation

M3 - Paper

SP - Page 26

ER -

Pollnau M, Hammer M, Dongre C, Hoekstra H. Sub-base-pair resolution during DNA separation in an optofluidic chip. 2014. Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.