Sub-base-pair resolution during DNA separation in an optofluidic chip

Markus Pollnau, Manfred Hammer, C. Dongre, Hugo Hoekstra

    Research output: Contribution to conferencePaper

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    Abstract

    DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.
    Original languageUndefined
    PagesPage 26
    Number of pages1
    Publication statusPublished - Sep 2014
    Event40th International Conference on Micro & Nano Engineering, MNE 2014 - Lausanne, Switzerland
    Duration: 22 Sep 201426 Sep 2014
    Conference number: 40

    Conference

    Conference40th International Conference on Micro & Nano Engineering, MNE 2014
    Abbreviated titleMNE
    CountrySwitzerland
    CityLausanne
    Period22/09/1426/09/14

    Keywords

    • Capillary electrophoresis
    • femtosecond laser writing
    • EWI-25107
    • microfluidic chip
    • IR-91972
    • IOMS-SNS: SENSORS
    • DNA separation

    Cite this

    Pollnau, M., Hammer, M., Dongre, C., & Hoekstra, H. (2014). Sub-base-pair resolution during DNA separation in an optofluidic chip. Page 26. Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.