Sub-base-pair resolution during DNA separation in an optofluidic chip

Markus Pollnau, Manfred Hammer, C. Dongre, Hugo Hoekstra

    Research output: Contribution to conferencePaper

    15 Downloads (Pure)

    Abstract

    DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.
    Original languageUndefined
    PagesPage 26
    Number of pages1
    Publication statusPublished - Sep 2014
    Event40th International Conference on Micro & Nano Engineering, MNE 2014 - Lausanne, Switzerland
    Duration: 22 Sep 201426 Sep 2014
    Conference number: 40

    Conference

    Conference40th International Conference on Micro & Nano Engineering, MNE 2014
    Abbreviated titleMNE
    CountrySwitzerland
    CityLausanne
    Period22/09/1426/09/14

    Keywords

    • Capillary electrophoresis
    • femtosecond laser writing
    • EWI-25107
    • microfluidic chip
    • IR-91972
    • IOMS-SNS: SENSORS
    • DNA separation

    Cite this

    Pollnau, M., Hammer, M., Dongre, C., & Hoekstra, H. (2014). Sub-base-pair resolution during DNA separation in an optofluidic chip. Page 26. Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.
    Pollnau, Markus ; Hammer, Manfred ; Dongre, C. ; Hoekstra, Hugo. / Sub-base-pair resolution during DNA separation in an optofluidic chip. Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.1 p.
    @conference{4b83bf054f214ac3be280f83c185cc02,
    title = "Sub-base-pair resolution during DNA separation in an optofluidic chip",
    abstract = "DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.",
    keywords = "Capillary electrophoresis, femtosecond laser writing, EWI-25107, microfluidic chip, IR-91972, IOMS-SNS: SENSORS, DNA separation",
    author = "Markus Pollnau and Manfred Hammer and C. Dongre and Hugo Hoekstra",
    year = "2014",
    month = "9",
    language = "Undefined",
    pages = "Page 26",
    note = "null ; Conference date: 22-09-2014 Through 26-09-2014",

    }

    Pollnau, M, Hammer, M, Dongre, C & Hoekstra, H 2014, 'Sub-base-pair resolution during DNA separation in an optofluidic chip' Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland, 22/09/14 - 26/09/14, pp. Page 26.

    Sub-base-pair resolution during DNA separation in an optofluidic chip. / Pollnau, Markus; Hammer, Manfred; Dongre, C.; Hoekstra, Hugo.

    2014. Page 26 Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.

    Research output: Contribution to conferencePaper

    TY - CONF

    T1 - Sub-base-pair resolution during DNA separation in an optofluidic chip

    AU - Pollnau, Markus

    AU - Hammer, Manfred

    AU - Dongre, C.

    AU - Hoekstra, Hugo

    PY - 2014/9

    Y1 - 2014/9

    N2 - DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.

    AB - DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.

    KW - Capillary electrophoresis

    KW - femtosecond laser writing

    KW - EWI-25107

    KW - microfluidic chip

    KW - IR-91972

    KW - IOMS-SNS: SENSORS

    KW - DNA separation

    M3 - Paper

    SP - Page 26

    ER -

    Pollnau M, Hammer M, Dongre C, Hoekstra H. Sub-base-pair resolution during DNA separation in an optofluidic chip. 2014. Paper presented at 40th International Conference on Micro & Nano Engineering, MNE 2014 , Lausanne, Switzerland.