Sub-base-pair resolution during DNA separation in an optofluidic chip

Markus Pollnau; Manfred Hammer; C. Dongre; Hugo Hoekstra

Sub-base-pair resolution during DNA separation in an optofluidic chip

Markus Pollnau
, Manfred Hammer
, C. Dongre
, Hugo Hoekstra

Research output: Contribution to conference › Paper

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Abstract

DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.

Original language	Undefined
Pages	Page 26
Number of pages	1
Publication status	Published - Sept 2014
Event	40th International Conference on Micro & Nano Engineering, MNE 2014 - Lausanne, Switzerland Duration: 22 Sept 2014 → 26 Sept 2014 Conference number: 40

Conference

Conference	40th International Conference on Micro & Nano Engineering, MNE 2014
Abbreviated title	MNE
Country/Territory	Switzerland
City	Lausanne
Period	22/09/14 → 26/09/14

Keywords

Capillary electrophoresis
femtosecond laser writing
EWI-25107
microfluidic chip
IR-91972
IOMS-SNS: SENSORS
DNA separation

Access to Document

MNE2014_Pollnau

http://eprints.eemcs.utwente.nl/secure2/25107/01/MNE2014_Pollnau.pdf

Cite this

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title = "Sub-base-pair resolution during DNA separation in an optofluidic chip",

abstract = "DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10\textasciicircum{}-3, while only S < 10\textasciicircum{}-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10\textasciicircum{}-4, thereby paving the way for the envisaged applications.",

keywords = "Capillary electrophoresis, femtosecond laser writing, EWI-25107, microfluidic chip, IR-91972, IOMS-SNS: SENSORS, DNA separation",

author = "Markus Pollnau and Manfred Hammer and C. Dongre and Hugo Hoekstra",

year = "2014",

month = sep,

language = "Undefined",

pages = "Page 26",

note = "40th International Conference on Micro \& Nano Engineering, MNE 2014 ; Conference date: 22-09-2014 Through 26-09-2014",

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TY - CONF

T1 - Sub-base-pair resolution during DNA separation in an optofluidic chip

AU - Pollnau, Markus

AU - Hammer, Manfred

AU - Dongre, C.

AU - Hoekstra, Hugo

N1 - Conference code: 40

PY - 2014/9

Y1 - 2014/9

N2 - DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.

AB - DNA sequencing in a lab-on-a-chip aims at providing cheap, high-speed analysis of low reagent volumes to, e.g., identify genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant range of 150-1000 base-pairs requires a sizing accuracy of S < 10^-3, while only S < 10^-2 were reported. Here we demonstrate a sizing accuracy of S = 4 x 10^-4, thereby paving the way for the envisaged applications.

KW - Capillary electrophoresis

KW - femtosecond laser writing

KW - EWI-25107

KW - microfluidic chip

KW - IR-91972

KW - IOMS-SNS: SENSORS

KW - DNA separation

M3 - Paper

SP - Page 26

T2 - 40th International Conference on Micro & Nano Engineering, MNE 2014

Y2 - 22 September 2014 through 26 September 2014

ER -