Sub-Diffraction Resolution CARS Microscopy via Rabi Modulation: a Theoretical Investigation

Willem P. Beeker, Petra Groß, Chris J. Lee, Carsten Cleff, Herman L. Offerhaus, Carsten Fallnich, Jennifer L. Herek, Klaus-J. Boller

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Label-free imaging modalities, such as coherent anti-Stokes Raman spectroscopy (CARS) microscopy, have demonstrated high contrast levels and video rate image acquisition times. CARS is a nonlinear process where two lasers coherently populate a vibrational state due to a two-photon resonant interaction. A probe laser coherently depopulates the excited state, generating light at the anti-Stokes frequency, which is collected and imaged. We show that the spatial resolution of CARS can be enhanced by modulating the ground state coherence. The resulting modulation of the CARS emission intensity introduces side bands at the Rabi frequency, to the CARS spectra. The additional laser when focused to diffraction limited Gaussian shaped intensity profile will generate a distribution of Rabi side bands that depend on the radial distance from the center of the focus. By accurately measuring the amplitudes of the side bands as the laser focus is scanned, a sub-diffraction resolution image can be constructed.
Original languageEnglish
Title of host publicationProceedings Cleo-Europe - EQEC 2009
Place of PublicationPiscataway, NJ
PublisherIEEE
Number of pages1
ISBN (Electronic)978-1-4244-4080-1
ISBN (Print)978-1-4244-4079-5
DOIs
Publication statusPublished - 14 Jun 2009
Event11th European Quantum Electronics Conference, EQEC 2009 - Munich, Germany
Duration: 14 Jun 200919 Jun 2009
Conference number: 11
http://2009.cleoeurope.org/

Conference

Conference11th European Quantum Electronics Conference, EQEC 2009
Abbreviated titleEQEC 2009
CountryGermany
CityMunich
Period14/06/0919/06/09
Internet address

Keywords

  • Microscopy
  • Focusing
  • Laser theory
  • Frequency
  • High-resolution imaging
  • Raman scattering
  • Spectroscopy
  • Resonance
  • Laser excitation
  • Probes

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