Subharmonic contrast intravascular ultrasound for vasa vasorum imaging

David E. Goertz, Martijn E. Frijlink, Dennie Tempel, Vijay Bhagwandas, Andries Gisolf, Robert Krams, N. de Jong, Antonius F.W. van der Steen

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The feasibility of subharmonic contrast intravascular ultrasound (IVUS) imaging was investigated using a prototype nonlinear IVUS system and the commercial contrast agent Definity™. The system employed a mechanically scanned commercial catheter with a custom transducer element fabricated to have sensitivity at both 15 and 30 MHz. Experiments were conducted at a fundamental frequency of 30 MHz (F30; 25% bandwidth), with on-axis pressures ranging from 0.12 to 0.79 MPa, as measured with a needle hydrophone. In vitro characterization experiments demonstrated the detection of 15 MHz subharmonic signals (SH15) when pressure levels reached 360 kPa. The formation of SH15 images was shown, with tissue signals suppressed to near the noise floor and contrast to tissue ratios were improved by up to 30 dB relative to F30. In vivo experiments were performed using the atherosclerotic rabbit aorta model. Following the bolus injection of contrast agent upstream of the imaging catheter, agent was detected within the aorta, vena cava and within the perivascular space. These results provide a first in vivo demonstration of subharmonic contrast IVUS and suggest its potential as a new technique for imaging vasa vasorum.
Original languageUndefined
Pages (from-to)1859-1872
Number of pages14
JournalUltrasound in medicine and biology
Issue number12
Publication statusPublished - 2007


  • METIS-244553
  • Vulnerable plaque
  • Subharmonic
  • Vasa vasorum
  • Non-linear
  • Microbubbles
  • Coronary artery
  • Intravascular ultrasound
  • Atherosclerosis
  • IR-78821
  • Contrast agents

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