TY - JOUR
T1 - Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo
AU - Davison, N.L.
AU - Luo, Xiaoman
AU - Schoenmaker, T.
AU - Everts, V.
AU - Yuan, Huipin
AU - Barrère-de Groot, F.
AU - de Bruijn, Joost Dick
N1 - Article. PubMed ID: 24733686
PY - 2014
Y1 - 2014
N2 - A current challenge of synthetic bone graft substitute design is to induce bone formation at a similar rate to its biological resorption, matching bone’s intrinsic osteoinductivity and capacity for remodelling. We hypothesise that both osteoinduction and resorption can be achieved by altering surface microstructure of beta-tricalcium phosphate (TCP). To test this, two TCP ceramics are engineered with equivalent chemistry and macrostructure but with either submicron- or micron-scale surface architecture. In vitro, submicron-scale surface architecture differentiates larger, more active osteoclasts – a cell type shown to be important for both TCP resorption and osteogenesis – and enhances their secretion of osteogenic factors to induce osteoblast differentiation of human mesenchymal stem cells. In an intramuscular model, submicrostructured TCP forms 20 % bone in the free space, is resorbed by 24 %, and is densely populated by multinucleated osteoclast-like cells after 12 weeks; however, TCP with micron-scale surface architecture forms no bone, is essentially not resorbed, and contains scarce osteoclast-like cells. Thus, a novel submicron-structured TCP induces substantial bone formation and is resorbed at an equivalent rate, potentially through the control of osteoclast-like cells.
AB - A current challenge of synthetic bone graft substitute design is to induce bone formation at a similar rate to its biological resorption, matching bone’s intrinsic osteoinductivity and capacity for remodelling. We hypothesise that both osteoinduction and resorption can be achieved by altering surface microstructure of beta-tricalcium phosphate (TCP). To test this, two TCP ceramics are engineered with equivalent chemistry and macrostructure but with either submicron- or micron-scale surface architecture. In vitro, submicron-scale surface architecture differentiates larger, more active osteoclasts – a cell type shown to be important for both TCP resorption and osteogenesis – and enhances their secretion of osteogenic factors to induce osteoblast differentiation of human mesenchymal stem cells. In an intramuscular model, submicrostructured TCP forms 20 % bone in the free space, is resorbed by 24 %, and is densely populated by multinucleated osteoclast-like cells after 12 weeks; however, TCP with micron-scale surface architecture forms no bone, is essentially not resorbed, and contains scarce osteoclast-like cells. Thus, a novel submicron-structured TCP induces substantial bone formation and is resorbed at an equivalent rate, potentially through the control of osteoclast-like cells.
KW - METIS-309537
KW - IR-95162
M3 - Article
VL - 27
SP - 281
EP - 297
JO - European cells & materials
JF - European cells & materials
SN - 1473-2262
ER -