Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

N.L. Davison, Xiaoman Luo, T. Schoenmaker, V. Everts, Huipin Yuan, F. Barrère-de Groot, Joost Dick de Bruijn

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

A current challenge of synthetic bone graft substitute design is to induce bone formation at a similar rate to its biological resorption, matching bone’s intrinsic osteoinductivity and capacity for remodelling. We hypothesise that both osteoinduction and resorption can be achieved by altering surface microstructure of beta-tricalcium phosphate (TCP). To test this, two TCP ceramics are engineered with equivalent chemistry and macrostructure but with either submicron- or micron-scale surface architecture. In vitro, submicron-scale surface architecture differentiates larger, more active osteoclasts – a cell type shown to be important for both TCP resorption and osteogenesis – and enhances their secretion of osteogenic factors to induce osteoblast differentiation of human mesenchymal stem cells. In an intramuscular model, submicrostructured TCP forms 20 % bone in the free space, is resorbed by 24 %, and is densely populated by multinucleated osteoclast-like cells after 12 weeks; however, TCP with micron-scale surface architecture forms no bone, is essentially not resorbed, and contains scarce osteoclast-like cells. Thus, a novel submicron-structured TCP induces substantial bone formation and is resorbed at an equivalent rate, potentially through the control of osteoclast-like cells.
Original languageUndefined
Pages (from-to)281-297
JournalEuropean cells & materials
Volume27
Publication statusPublished - 2014

Keywords

  • METIS-309537
  • IR-95162

Cite this

Davison, N. L., Luo, X., Schoenmaker, T., Everts, V., Yuan, H., Barrère-de Groot, F., & de Bruijn, J. D. (2014). Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo. European cells & materials, 27, 281-297.