Supercritical carbon dioxide decellularised pericardium: Mechanical and structural characterisation for applications in cardio-thoracic surgery

Frank Ruben Halfwerk (Corresponding Author), Jeroen Rouwkema, J. Gossen, Jan G Grandjean

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    3 Citations (Scopus)
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    Abstract

    Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,
    calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterise
    decellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biological
    material for uses in cardio-thoracic surgery.

    Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensile
    strength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure was
    visualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.
    Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.

    Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared to
    untreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehyde
    treated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs
    10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher than
    glutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treated
    pericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, while
    glutaraldehyde treated pericardium showed deterioration of extracellular matrix.

    Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovine
    pericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.
    Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery without
    compromising initial good mechanical properties.
    Original languageEnglish
    Pages (from-to)400
    Number of pages8
    JournalJournal of the mechanical behavior of biomedical materials
    Volume77
    Early online date3 Oct 2017
    DOIs
    Publication statusPublished - 1 Jan 2018

    Fingerprint

    Glutaral
    Carbon Dioxide
    Surgery
    Carbon dioxide
    Mechanical properties
    Tensile strength
    Processing
    Tissue
    Biocompatible Materials
    Biomaterials
    Swelling
    Deterioration
    Structural properties
    Fracture toughness
    Anisotropy
    Elastic moduli
    Scanning electron microscopy
    Water

    Keywords

    • Decellularisation
    • Extracellular matrix
    • Isotropy
    • Cardiovascular mechanics
    • Biomechanics

    Cite this

    @article{f8e9f4c35a8e4aeaa4ddef287302502a,
    title = "Supercritical carbon dioxide decellularised pericardium: Mechanical and structural characterisation for applications in cardio-thoracic surgery",
    abstract = "Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterisedecellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biologicalmaterial for uses in cardio-thoracic surgery.Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensilestrength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure wasvisualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared tountreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehydetreated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher thanglutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treatedpericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, whileglutaraldehyde treated pericardium showed deterioration of extracellular matrix.Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovinepericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery withoutcompromising initial good mechanical properties.",
    keywords = "Decellularisation, Extracellular matrix, Isotropy , Cardiovascular mechanics, Biomechanics",
    author = "Halfwerk, {Frank Ruben} and Jeroen Rouwkema and J. Gossen and Grandjean, {Jan G}",
    year = "2018",
    month = "1",
    day = "1",
    doi = "10.1016/j.jmbbm.2017.10.002",
    language = "English",
    volume = "77",
    pages = "400",
    journal = "Journal of the mechanical behavior of biomedical materials",
    issn = "1751-6161",
    publisher = "Elsevier",

    }

    TY - JOUR

    T1 - Supercritical carbon dioxide decellularised pericardium

    T2 - Mechanical and structural characterisation for applications in cardio-thoracic surgery

    AU - Halfwerk, Frank Ruben

    AU - Rouwkema, Jeroen

    AU - Gossen, J.

    AU - Grandjean, Jan G

    PY - 2018/1/1

    Y1 - 2018/1/1

    N2 - Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterisedecellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biologicalmaterial for uses in cardio-thoracic surgery.Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensilestrength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure wasvisualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared tountreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehydetreated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher thanglutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treatedpericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, whileglutaraldehyde treated pericardium showed deterioration of extracellular matrix.Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovinepericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery withoutcompromising initial good mechanical properties.

    AB - Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterisedecellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biologicalmaterial for uses in cardio-thoracic surgery.Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensilestrength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure wasvisualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared tountreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehydetreated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher thanglutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treatedpericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, whileglutaraldehyde treated pericardium showed deterioration of extracellular matrix.Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovinepericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery withoutcompromising initial good mechanical properties.

    KW - Decellularisation

    KW - Extracellular matrix

    KW - Isotropy

    KW - Cardiovascular mechanics

    KW - Biomechanics

    U2 - 10.1016/j.jmbbm.2017.10.002

    DO - 10.1016/j.jmbbm.2017.10.002

    M3 - Article

    VL - 77

    SP - 400

    JO - Journal of the mechanical behavior of biomedical materials

    JF - Journal of the mechanical behavior of biomedical materials

    SN - 1751-6161

    ER -