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Abstract
Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,
calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterise
decellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biological
material for uses in cardio-thoracic surgery.
Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensile
strength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure was
visualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.
Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.
Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared to
untreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehyde
treated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs
10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher than
glutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treated
pericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, while
glutaraldehyde treated pericardium showed deterioration of extracellular matrix.
Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovine
pericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.
Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery without
compromising initial good mechanical properties.
calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterise
decellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biological
material for uses in cardio-thoracic surgery.
Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensile
strength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure was
visualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.
Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.
Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared to
untreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehyde
treated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs
10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher than
glutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treated
pericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, while
glutaraldehyde treated pericardium showed deterioration of extracellular matrix.
Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovine
pericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.
Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery without
compromising initial good mechanical properties.
Original language | English |
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Pages (from-to) | 400 |
Number of pages | 8 |
Journal | Journal of the mechanical behavior of biomedical materials |
Volume | 77 |
Early online date | 3 Oct 2017 |
DOIs | |
Publication status | Published - 1 Jan 2018 |
Keywords
- Decellularisation
- Extracellular matrix
- Isotropy
- Cardiovascular mechanics
- Biomechanics
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Biomechanical Engineering meetings from October 2014 to July 2023: The heart of Biomechanical Engineering
Halfwerk, F. R. (Speaker)
6 Jul 2023Activity: Talk or presentation › Oral presentation