Supercritical carbon dioxide decellularised pericardium: Mechanical and structural characterisation for applications in cardio-thoracic surgery

Frank Ruben Halfwerk (Corresponding Author), Jeroen Rouwkema, J. Gossen, Jan G Grandjean

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,
calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterise
decellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biological
material for uses in cardio-thoracic surgery.

Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensile
strength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure was
visualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.
Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.

Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared to
untreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehyde
treated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs
10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher than
glutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treated
pericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, while
glutaraldehyde treated pericardium showed deterioration of extracellular matrix.

Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovine
pericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.
Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery without
compromising initial good mechanical properties.
Original languageEnglish
Pages (from-to)400
Number of pages8
JournalJournal of the mechanical behavior of biomedical materials
Volume77
Early online date3 Oct 2017
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Glutaral
Carbon Dioxide
Surgery
Carbon dioxide
Mechanical properties
Tensile strength
Processing
Tissue
Biocompatible Materials
Biomaterials
Swelling
Deterioration
Structural properties
Fracture toughness
Anisotropy
Elastic moduli
Scanning electron microscopy
Water

Keywords

  • Decellularisation
  • Extracellular matrix
  • Isotropy
  • Cardiovascular mechanics
  • Biomechanics

Cite this

@article{f8e9f4c35a8e4aeaa4ddef287302502a,
title = "Supercritical carbon dioxide decellularised pericardium: Mechanical and structural characterisation for applications in cardio-thoracic surgery",
abstract = "Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterisedecellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biologicalmaterial for uses in cardio-thoracic surgery.Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensilestrength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure wasvisualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared tountreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehydetreated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher thanglutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treatedpericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, whileglutaraldehyde treated pericardium showed deterioration of extracellular matrix.Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovinepericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery withoutcompromising initial good mechanical properties.",
keywords = "Decellularisation, Extracellular matrix, Isotropy , Cardiovascular mechanics, Biomechanics",
author = "Halfwerk, {Frank Ruben} and Jeroen Rouwkema and J. Gossen and Grandjean, {Jan G}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.jmbbm.2017.10.002",
language = "English",
volume = "77",
pages = "400",
journal = "Journal of the mechanical behavior of biomedical materials",
issn = "1751-6161",
publisher = "Elsevier",

}

TY - JOUR

T1 - Supercritical carbon dioxide decellularised pericardium

T2 - Mechanical and structural characterisation for applications in cardio-thoracic surgery

AU - Halfwerk, Frank Ruben

AU - Rouwkema, Jeroen

AU - Gossen, J.

AU - Grandjean, Jan G

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterisedecellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biologicalmaterial for uses in cardio-thoracic surgery.Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensilestrength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure wasvisualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared tountreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehydetreated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher thanglutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treatedpericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, whileglutaraldehyde treated pericardium showed deterioration of extracellular matrix.Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovinepericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery withoutcompromising initial good mechanical properties.

AB - Introduction: Many biomaterials are used in cardio-thoracic surgery with good short-term results. However,calcification, dehiscence, and formation of scar tissue are reported. The aim of this research is to characterisedecellularised pericardium after supercritical carbon dioxide (scCO2) processing as an alternative biologicalmaterial for uses in cardio-thoracic surgery.Methods: Porcine and bovine pericardium were decellularised using scCO2. Mechanical properties such as tensilestrength, elastic modulus, fracture toughness and suture retention strength were determined. Ultrastructure wasvisualised using Scanning Electron Microscopy. Water uptake and swelling was experimentally determined.Commercially available glutaraldehyde treated bovine pericardium was used as gold standard for comparison.Results: scCO2 decellularised porcine (and bovine pericardium) maintained their tensile strength compared tountreated native pericardium (13.3±2.4 MPa vs 14.0±4.1 MPa, p =0.73). Tensile strength of glutaraldehydetreated pericardium was significantly higher compared to untreated pericardium (19.4±7.3 MPa vs10.2±2.2 MPa, p=0.02). Suture retention strength of scCO2 treated pericardium was significantly higher thanglutaraldehyde treated pericardium (p = 0.01). We found no anisotropy of scCO2 or glutaraldehyde treatedpericardium based on a trouser tear test. Ultrastructure was uncompromised in scCO2 treated pericardium, whileglutaraldehyde treated pericardium showed deterioration of extracellular matrix.Conclusion: scCO2 processing preserves initial mechanical and structural properties of porcine and bovinepericardium, while glutaraldehyde processing damages the extracellular matrix of bovine pericardium.Decellularisation of tissue using scCO2 might give long-term solutions for cardio-thoracic surgery withoutcompromising initial good mechanical properties.

KW - Decellularisation

KW - Extracellular matrix

KW - Isotropy

KW - Cardiovascular mechanics

KW - Biomechanics

U2 - 10.1016/j.jmbbm.2017.10.002

DO - 10.1016/j.jmbbm.2017.10.002

M3 - Article

VL - 77

SP - 400

JO - Journal of the mechanical behavior of biomedical materials

JF - Journal of the mechanical behavior of biomedical materials

SN - 1751-6161

ER -