Surface plasmon resonance immunosensors: sensitivity considerations

R.P.H. Kooyman; H. Kolkman; J. van Gent; Jan Greve

doi:10.1016/S0003-2670(00)81337-9

Surface plasmon resonance immunosensors: sensitivity considerations

R.P.H. Kooyman, H. Kolkman, J. van Gent, Jan Greve

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Abstract

Some aspects of improving surface plasmon resonance response in immunosensing applications are considered. Both from calculations and experiments, it was found that maximum sensitivity is obtained for a silver layer about 55 nm thick in direct contact with the species to be quantified. Application of an intermediate layer with high permittivity can be useful in suppressing background responses. Experimentally, a protein surface-coverage fraction of ca. 0.1 could be measured, corresponding to ca. 10−10 mol1−1 antibody.

Original language	Undefined
Pages (from-to)	35-45
Journal	Analytica chimica acta
Volume	213
DOIs	https://doi.org/10.1016/S0003-2670(00)81337-9
Publication status	Published - 1988

Keywords

IR-70325

Access to Document

10.1016/S0003-2670(00)81337-9

Kooyman88surface

Cite this

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title = "Surface plasmon resonance immunosensors: sensitivity considerations",

abstract = "Some aspects of improving surface plasmon resonance response in immunosensing applications are considered. Both from calculations and experiments, it was found that maximum sensitivity is obtained for a silver layer about 55 nm thick in direct contact with the species to be quantified. Application of an intermediate layer with high permittivity can be useful in suppressing background responses. Experimentally, a protein surface-coverage fraction of ca. 0.1 could be measured, corresponding to ca. 10−10 mol1−1 antibody.",

keywords = "IR-70325",

author = "R.P.H. Kooyman and H. Kolkman and {van Gent}, J. and Jan Greve",

year = "1988",

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T1 - Surface plasmon resonance immunosensors: sensitivity considerations

AU - Kooyman, R.P.H.

AU - Kolkman, H.

AU - van Gent, J.

AU - Greve, Jan

PY - 1988

Y1 - 1988

N2 - Some aspects of improving surface plasmon resonance response in immunosensing applications are considered. Both from calculations and experiments, it was found that maximum sensitivity is obtained for a silver layer about 55 nm thick in direct contact with the species to be quantified. Application of an intermediate layer with high permittivity can be useful in suppressing background responses. Experimentally, a protein surface-coverage fraction of ca. 0.1 could be measured, corresponding to ca. 10−10 mol1−1 antibody.

AB - Some aspects of improving surface plasmon resonance response in immunosensing applications are considered. Both from calculations and experiments, it was found that maximum sensitivity is obtained for a silver layer about 55 nm thick in direct contact with the species to be quantified. Application of an intermediate layer with high permittivity can be useful in suppressing background responses. Experimentally, a protein surface-coverage fraction of ca. 0.1 could be measured, corresponding to ca. 10−10 mol1−1 antibody.

KW - IR-70325

U2 - 10.1016/S0003-2670(00)81337-9

DO - 10.1016/S0003-2670(00)81337-9

M3 - Article

SN - 0003-2670

VL - 213

SP - 35

EP - 45

JO - Analytica chimica acta

JF - Analytica chimica acta

ER -