TY - JOUR
T1 - Surface plasmon resonance immunosensors: sensitivity considerations
AU - Kooyman, R.P.H.
AU - Kolkman, H.
AU - van Gent, J.
AU - Greve, Jan
PY - 1988
Y1 - 1988
N2 - Some aspects of improving surface plasmon resonance response in immunosensing applications are considered. Both from calculations and experiments, it was found that maximum sensitivity is obtained for a silver layer about 55 nm thick in direct contact with the species to be quantified. Application of an intermediate layer with high permittivity can be useful in suppressing background responses. Experimentally, a protein surface-coverage fraction of ca. 0.1 could be measured, corresponding to ca. 10−10 mol1−1 antibody.
AB - Some aspects of improving surface plasmon resonance response in immunosensing applications are considered. Both from calculations and experiments, it was found that maximum sensitivity is obtained for a silver layer about 55 nm thick in direct contact with the species to be quantified. Application of an intermediate layer with high permittivity can be useful in suppressing background responses. Experimentally, a protein surface-coverage fraction of ca. 0.1 could be measured, corresponding to ca. 10−10 mol1−1 antibody.
KW - IR-70325
U2 - 10.1016/S0003-2670(00)81337-9
DO - 10.1016/S0003-2670(00)81337-9
M3 - Article
SN - 0003-2670
VL - 213
SP - 35
EP - 45
JO - Analytica chimica acta
JF - Analytica chimica acta
ER -