Some aspects of improving surface plasmon resonance response in immunosensing applications are considered. Both from calculations and experiments, it was found that maximum sensitivity is obtained for a silver layer about 55 nm thick in direct contact with the species to be quantified. Application of an intermediate layer with high permittivity can be useful in suppressing background responses. Experimentally, a protein surface-coverage fraction of ca. 0.1 could be measured, corresponding to ca. 10−10 mol1−1 antibody.