Surfactant-free Preparation of Highly Stable Zwitterionic Poly(amido amine) Nanogels with Minimal Cytotoxicity

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Abstract

Narrowly dispersed zwitterionic poly(amido amine) (PAA) nanogels with a diameter of approximately 100 nm were prepared by a high-yielding and surfactant-free, inverse nanoprecipitation of PAA polymers. The resulting, negatively charged, nanogels (PAA-NG1) were functionalized with N,N-dimethylethylenediamine via EDC/NHS coupling chemistry. This resulted in nanogels with a positive surface charge (PAA-NG2). Both types of nanogels were fluorescently labelled via isothiocyanate coupling. PAA-NG1 displays high colloidal stability both in PBS and Fetal Bovine Serum solution. Moreover, both nanogels exhibit a distinct zwitterionic swelling profile in response to pH changes. Cellular uptake of FITC-labelled nanogels with RAW 264.7, PC-3 and COS-7 cells was evaluated by fluorescence microscopy. These studies showed that nanogel surface charge greatly influences nanogel–cell interactions. The PAA polymer and PAA-NG1 showed minimal cell toxicity as was evaluated by MTT assays. The findings reported here demonstrate that PAA nanogels possess interesting properties for future studies in both drug delivery and imaging.
Original languageEnglish
Pages (from-to)126-134
JournalActa biomaterialia
Volume30
DOIs
Publication statusPublished - 2016

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Cytotoxicity
Surface charge
Surface-Active Agents
Amines
Surface active agents
Fluorescence microscopy
Polymers
Drug delivery
Toxicity
Swelling
Assays
Imaging techniques
NanoGel
Fluorescein-5-isothiocyanate
COS Cells
Fluorescence Microscopy
Serum
Pharmaceutical Preparations

Keywords

  • IR-99423
  • METIS-313819

Cite this

@article{d2eee2921325450bb2742919d6431127,
title = "Surfactant-free Preparation of Highly Stable Zwitterionic Poly(amido amine) Nanogels with Minimal Cytotoxicity",
abstract = "Narrowly dispersed zwitterionic poly(amido amine) (PAA) nanogels with a diameter of approximately 100 nm were prepared by a high-yielding and surfactant-free, inverse nanoprecipitation of PAA polymers. The resulting, negatively charged, nanogels (PAA-NG1) were functionalized with N,N-dimethylethylenediamine via EDC/NHS coupling chemistry. This resulted in nanogels with a positive surface charge (PAA-NG2). Both types of nanogels were fluorescently labelled via isothiocyanate coupling. PAA-NG1 displays high colloidal stability both in PBS and Fetal Bovine Serum solution. Moreover, both nanogels exhibit a distinct zwitterionic swelling profile in response to pH changes. Cellular uptake of FITC-labelled nanogels with RAW 264.7, PC-3 and COS-7 cells was evaluated by fluorescence microscopy. These studies showed that nanogel surface charge greatly influences nanogel–cell interactions. The PAA polymer and PAA-NG1 showed minimal cell toxicity as was evaluated by MTT assays. The findings reported here demonstrate that PAA nanogels possess interesting properties for future studies in both drug delivery and imaging.",
keywords = "IR-99423, METIS-313819",
author = "A.E. Ekkelenkamp and M.M.T. Jansman and Roelofs-Haarhuis, {Hendrika Maria} and Engbersen, {Johannes F.J.} and Paulusse, {Jos Marie Johannes}",
year = "2016",
doi = "10.1016/j.actbio.2015.10.037",
language = "English",
volume = "30",
pages = "126--134",
journal = "Acta biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",

}

Surfactant-free Preparation of Highly Stable Zwitterionic Poly(amido amine) Nanogels with Minimal Cytotoxicity. / Ekkelenkamp, A.E.; Jansman, M.M.T.; Roelofs-Haarhuis, Hendrika Maria; Engbersen, Johannes F.J.; Paulusse, Jos Marie Johannes.

In: Acta biomaterialia, Vol. 30, 2016, p. 126-134.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Surfactant-free Preparation of Highly Stable Zwitterionic Poly(amido amine) Nanogels with Minimal Cytotoxicity

AU - Ekkelenkamp, A.E.

AU - Jansman, M.M.T.

AU - Roelofs-Haarhuis, Hendrika Maria

AU - Engbersen, Johannes F.J.

AU - Paulusse, Jos Marie Johannes

PY - 2016

Y1 - 2016

N2 - Narrowly dispersed zwitterionic poly(amido amine) (PAA) nanogels with a diameter of approximately 100 nm were prepared by a high-yielding and surfactant-free, inverse nanoprecipitation of PAA polymers. The resulting, negatively charged, nanogels (PAA-NG1) were functionalized with N,N-dimethylethylenediamine via EDC/NHS coupling chemistry. This resulted in nanogels with a positive surface charge (PAA-NG2). Both types of nanogels were fluorescently labelled via isothiocyanate coupling. PAA-NG1 displays high colloidal stability both in PBS and Fetal Bovine Serum solution. Moreover, both nanogels exhibit a distinct zwitterionic swelling profile in response to pH changes. Cellular uptake of FITC-labelled nanogels with RAW 264.7, PC-3 and COS-7 cells was evaluated by fluorescence microscopy. These studies showed that nanogel surface charge greatly influences nanogel–cell interactions. The PAA polymer and PAA-NG1 showed minimal cell toxicity as was evaluated by MTT assays. The findings reported here demonstrate that PAA nanogels possess interesting properties for future studies in both drug delivery and imaging.

AB - Narrowly dispersed zwitterionic poly(amido amine) (PAA) nanogels with a diameter of approximately 100 nm were prepared by a high-yielding and surfactant-free, inverse nanoprecipitation of PAA polymers. The resulting, negatively charged, nanogels (PAA-NG1) were functionalized with N,N-dimethylethylenediamine via EDC/NHS coupling chemistry. This resulted in nanogels with a positive surface charge (PAA-NG2). Both types of nanogels were fluorescently labelled via isothiocyanate coupling. PAA-NG1 displays high colloidal stability both in PBS and Fetal Bovine Serum solution. Moreover, both nanogels exhibit a distinct zwitterionic swelling profile in response to pH changes. Cellular uptake of FITC-labelled nanogels with RAW 264.7, PC-3 and COS-7 cells was evaluated by fluorescence microscopy. These studies showed that nanogel surface charge greatly influences nanogel–cell interactions. The PAA polymer and PAA-NG1 showed minimal cell toxicity as was evaluated by MTT assays. The findings reported here demonstrate that PAA nanogels possess interesting properties for future studies in both drug delivery and imaging.

KW - IR-99423

KW - METIS-313819

U2 - 10.1016/j.actbio.2015.10.037

DO - 10.1016/j.actbio.2015.10.037

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JO - Acta biomaterialia

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SN - 1742-7061

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