Survival of adhering cortical neurons on polyethylenimine micropatterns

T.G. Ruardij, M.H. Goedbloed, Wim Rutten

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

The influence of neuron-adhesive pattern geometry on long-term survival of cortical neural tissue (rat brain) was studied over a time period of 15 days. Microwells (depth 0.5 /spl mu/m) with diameters of 25, 50, 100 and 150 /spl mu/m and inter-microwell distances of 15, 30, 60 and 90 /spl mu/m, were etched in a neuron-repellent fluorocarbon (FC)-layer and coated with neuron-adhesive polyethylenimine (PEI). Results showed that the survival of neural tissue was geometry-independent after 1, 4 and 8 days but was favored on 150 /spl mu/m wells after 15 days.
Original languageUndefined
Title of host publicationProceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Isyanbul, Turkey
Place of PublicationPiscattaway, USA
PublisherIEEE
Pages-
Number of pages4
ISBN (Print)0-7803-7213-1
Publication statusPublished - 25 Oct 2001
Event23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2001 - Istanbul, Turkey
Duration: 25 Oct 200128 Oct 2001
Conference number: 23

Publication series

Name
PublisherIEEE
Volume1

Conference

Conference23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2001
Abbreviated titleEMBC
CountryTurkey
CityIstanbul
Period25/10/0128/10/01

Keywords

  • METIS-200709
  • IR-42182

Cite this

Ruardij, T. G., Goedbloed, M. H., & Rutten, W. (2001). Survival of adhering cortical neurons on polyethylenimine micropatterns. In Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Isyanbul, Turkey (pp. -). Piscattaway, USA: IEEE.
Ruardij, T.G. ; Goedbloed, M.H. ; Rutten, Wim. / Survival of adhering cortical neurons on polyethylenimine micropatterns. Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Isyanbul, Turkey. Piscattaway, USA : IEEE, 2001. pp. -
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Ruardij, TG, Goedbloed, MH & Rutten, W 2001, Survival of adhering cortical neurons on polyethylenimine micropatterns. in Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Isyanbul, Turkey. IEEE, Piscattaway, USA, pp. -, 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2001, Istanbul, Turkey, 25/10/01.

Survival of adhering cortical neurons on polyethylenimine micropatterns. / Ruardij, T.G.; Goedbloed, M.H.; Rutten, Wim.

Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Isyanbul, Turkey. Piscattaway, USA : IEEE, 2001. p. -.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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N2 - The influence of neuron-adhesive pattern geometry on long-term survival of cortical neural tissue (rat brain) was studied over a time period of 15 days. Microwells (depth 0.5 /spl mu/m) with diameters of 25, 50, 100 and 150 /spl mu/m and inter-microwell distances of 15, 30, 60 and 90 /spl mu/m, were etched in a neuron-repellent fluorocarbon (FC)-layer and coated with neuron-adhesive polyethylenimine (PEI). Results showed that the survival of neural tissue was geometry-independent after 1, 4 and 8 days but was favored on 150 /spl mu/m wells after 15 days.

AB - The influence of neuron-adhesive pattern geometry on long-term survival of cortical neural tissue (rat brain) was studied over a time period of 15 days. Microwells (depth 0.5 /spl mu/m) with diameters of 25, 50, 100 and 150 /spl mu/m and inter-microwell distances of 15, 30, 60 and 90 /spl mu/m, were etched in a neuron-repellent fluorocarbon (FC)-layer and coated with neuron-adhesive polyethylenimine (PEI). Results showed that the survival of neural tissue was geometry-independent after 1, 4 and 8 days but was favored on 150 /spl mu/m wells after 15 days.

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Ruardij TG, Goedbloed MH, Rutten W. Survival of adhering cortical neurons on polyethylenimine micropatterns. In Proceedings 23rd International Conference of the IEEE Engineering in Medicine and Biology Society, Isyanbul, Turkey. Piscattaway, USA: IEEE. 2001. p. -