Synchronized, continuous-flow zone electrophoresis

D.R. Zalewski, D. Kohlheyer, Stefan Schlautmann, Johannes G.E. Gardeniers

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

A new method for performing continuous electrophoretic separation of complex mixtures in microscale devices is proposed. Unlike in free-flow electrophoresis devices, no mechanical pumping is requiredboth fluid transport and separation are driven electrokinetically. This gives the method great potential for on-a-chip integration in multistep analytical systems. The method enables us to collect fractionated sample and tensfold purification is possible. The model of the operation is presented and a detailed description of the optimal conditions for performing purification is given. The chip devices with 10-μm-deep separation chamber of 1.5 mm × 4 mm in size were fabricated in glass. A standard microchip electrophoresis setup was used. Continuous separation of rhodamine B, rhodamine 6G, and fluorescein was accomplished. Purification was demonstrated on a mixture containing rhodamine B and fluorescein, and the recovery of both fractions was achieved. The results show the feasibility of the method.
Original languageUndefined
Pages (from-to)6228-6234
Number of pages7
JournalAnalytical chemistry
Volume80
Issue number16
DOIs
Publication statusPublished - 2008

Keywords

  • IR-60356
  • METIS-249131

Cite this

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Synchronized, continuous-flow zone electrophoresis. / Zalewski, D.R.; Kohlheyer, D.; Schlautmann, Stefan; Gardeniers, Johannes G.E.

In: Analytical chemistry, Vol. 80, No. 16, 2008, p. 6228-6234.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Synchronized, continuous-flow zone electrophoresis

AU - Zalewski, D.R.

AU - Kohlheyer, D.

AU - Schlautmann, Stefan

AU - Gardeniers, Johannes G.E.

PY - 2008

Y1 - 2008

N2 - A new method for performing continuous electrophoretic separation of complex mixtures in microscale devices is proposed. Unlike in free-flow electrophoresis devices, no mechanical pumping is requiredboth fluid transport and separation are driven electrokinetically. This gives the method great potential for on-a-chip integration in multistep analytical systems. The method enables us to collect fractionated sample and tensfold purification is possible. The model of the operation is presented and a detailed description of the optimal conditions for performing purification is given. The chip devices with 10-μm-deep separation chamber of 1.5 mm × 4 mm in size were fabricated in glass. A standard microchip electrophoresis setup was used. Continuous separation of rhodamine B, rhodamine 6G, and fluorescein was accomplished. Purification was demonstrated on a mixture containing rhodamine B and fluorescein, and the recovery of both fractions was achieved. The results show the feasibility of the method.

AB - A new method for performing continuous electrophoretic separation of complex mixtures in microscale devices is proposed. Unlike in free-flow electrophoresis devices, no mechanical pumping is requiredboth fluid transport and separation are driven electrokinetically. This gives the method great potential for on-a-chip integration in multistep analytical systems. The method enables us to collect fractionated sample and tensfold purification is possible. The model of the operation is presented and a detailed description of the optimal conditions for performing purification is given. The chip devices with 10-μm-deep separation chamber of 1.5 mm × 4 mm in size were fabricated in glass. A standard microchip electrophoresis setup was used. Continuous separation of rhodamine B, rhodamine 6G, and fluorescein was accomplished. Purification was demonstrated on a mixture containing rhodamine B and fluorescein, and the recovery of both fractions was achieved. The results show the feasibility of the method.

KW - IR-60356

KW - METIS-249131

U2 - 10.1021/ac800567n

DO - 10.1021/ac800567n

M3 - Article

VL - 80

SP - 6228

EP - 6234

JO - Analytical chemistry

JF - Analytical chemistry

SN - 0003-2700

IS - 16

ER -