Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles

Agustin Enciso Martinez*, E. van der Pol, Aufried T.M. Lenferink, L.W.M.M. Terstappen, Ton van Leeuwen, Cornelis Otto

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)
85 Downloads (Pure)

Abstract

Extracellular Vesicles (EVs) can be used as biomarkers in diseases like cancer, as their lineage of origin and molecular composition depend on the presence of cancer cells. Recognition of tumor-derived EVs (tdEVs) from other particles and EVs in body fluids requires characterization of single EVs to exploit their biomarker potential. We present here a new method based on synchronized Rayleigh and Raman light scattering from a single laser beam, which optically traps single EVs. Rapidly measured sequences of the Rayleigh scattering amplitude show precisely when an individual EV is trapped and the synchronously acquired Raman spectrum labels every time interval with chemical information. Raman spectra of many single EVs can thus be acquired with great fidelity in an automated manner by blocking the laser beam at regular time intervals. This new method enables single EV characterization from fluids at the single particle level.
Original languageEnglish
Article number102109
JournalNanomedicine : nanotechnology, biology and medicine
Volume24
Early online date24 Oct 2019
DOIs
Publication statusPublished - Feb 2020

Keywords

  • UT-Hybrid-D

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