Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques

R.G. Rayavarapu, Wilhelmina Petersen, C. Ungureanu, Janine Nicole Post, Ton van Leeuwen, Srirang Manohar

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Abstract

We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.
Original languageUndefined
Pages (from-to)29817-1-29817-10
Number of pages10
JournalInternational Journal of Biomedical Imaging
DOIs
Publication statusPublished - 2007

Keywords

  • METIS-243201
  • IR-71157

Cite this

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title = "Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques",
abstract = "We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.",
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author = "R.G. Rayavarapu and Wilhelmina Petersen and C. Ungureanu and Post, {Janine Nicole} and {van Leeuwen}, Ton and Srirang Manohar",
note = "Open Access",
year = "2007",
doi = "10.1155/2007/29817",
language = "Undefined",
pages = "29817--1--29817--10",
journal = "International Journal of Biomedical Imaging",
issn = "1687-4188",
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Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques. / Rayavarapu, R.G.; Petersen, Wilhelmina; Ungureanu, C.; Post, Janine Nicole; van Leeuwen, Ton; Manohar, Srirang.

In: International Journal of Biomedical Imaging, 2007, p. 29817-1-29817-10.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques

AU - Rayavarapu, R.G.

AU - Petersen, Wilhelmina

AU - Ungureanu, C.

AU - Post, Janine Nicole

AU - van Leeuwen, Ton

AU - Manohar, Srirang

N1 - Open Access

PY - 2007

Y1 - 2007

N2 - We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

AB - We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.

KW - METIS-243201

KW - IR-71157

U2 - 10.1155/2007/29817

DO - 10.1155/2007/29817

M3 - Article

SP - 29817-1-29817-10

JO - International Journal of Biomedical Imaging

JF - International Journal of Biomedical Imaging

SN - 1687-4188

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