Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release

Elaheh Jooybar, Mohammad J. Abdekhodaie, Pieter J. Dijkstra

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Microgels are hydrophilic polymer matrix with high water content suitable for encapsulation and delivery of biomolecules. In this study, hyaluronic acid (HA) microgels were synthesized using a water in oil emulsion method. First, hyaluronic acid was modified with tyramine, and then the microemulsion was produced by homogenizing the polymer solution in isooctane as an oil phase. HA microdroplets were crosslinked via enzymatic method by addition of horseradish peroxidase (enzyme) and hydrogen peroxide, and stable microgels were produced in a mild crosslinking reaction. According to the results, larger microgels were achieved by increasing the initial polymer concentration. Two sample proteins, Bovine serum albumin (BSA) and lysozyme, were incorporated in the polymer network to investigate the encapsulation efficiency of the microgels. The results demonstrated that the proposed method has a high efficiency for protein encapsulation (> 70%). The release profiles showed that lysozyme, as a cationic protein, was released in a sustained manner over a period of two weeks. However, BSA, as a negatively charged protein, showed a faster release rate. The simple method of microgel fabrication, besides the sustained release of the encapsulated proteins, makes the HA microgels a promising vehicle for delivery of cationic proteins.

Original languageEnglish
Title of host publication2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018
Place of PublicationPiscataway, NJ
PublisherIEEE
Number of pages5
ISBN (Electronic)978-1-5386-7952-4
DOIs
Publication statusPublished - 2 Jul 2018
Event2018 25th National and 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018 - Qom, Iran, Islamic Republic of
Duration: 29 Nov 201830 Nov 2018
Conference number: 25

Publication series

Name2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018

Conference

Conference2018 25th National and 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018
Abbreviated titleICBME
CountryIran, Islamic Republic of
CityQom
Period29/11/1830/11/18

Fingerprint

Hyaluronic acid
Proteins
Encapsulation
Enzymes
Microemulsions
Biomolecules
Polymers
Polymer solutions
Polymer matrix
Hydrogen peroxide
Crosslinking
Water content
Emulsions
Fabrication
Water

Keywords

  • Enzymatic crosslinking
  • Hyaluronic acid
  • Inverse microemulsion
  • Microgels
  • Protein delivery

Cite this

Jooybar, E., Abdekhodaie, M. J., & Dijkstra, P. J. (2018). Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release. In 2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018 [8703588] (2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018). Piscataway, NJ: IEEE. https://doi.org/10.1109/ICBME.2018.8703588
Jooybar, Elaheh ; Abdekhodaie, Mohammad J. ; Dijkstra, Pieter J. / Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release. 2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018. Piscataway, NJ : IEEE, 2018. (2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018).
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abstract = "Microgels are hydrophilic polymer matrix with high water content suitable for encapsulation and delivery of biomolecules. In this study, hyaluronic acid (HA) microgels were synthesized using a water in oil emulsion method. First, hyaluronic acid was modified with tyramine, and then the microemulsion was produced by homogenizing the polymer solution in isooctane as an oil phase. HA microdroplets were crosslinked via enzymatic method by addition of horseradish peroxidase (enzyme) and hydrogen peroxide, and stable microgels were produced in a mild crosslinking reaction. According to the results, larger microgels were achieved by increasing the initial polymer concentration. Two sample proteins, Bovine serum albumin (BSA) and lysozyme, were incorporated in the polymer network to investigate the encapsulation efficiency of the microgels. The results demonstrated that the proposed method has a high efficiency for protein encapsulation (> 70{\%}). The release profiles showed that lysozyme, as a cationic protein, was released in a sustained manner over a period of two weeks. However, BSA, as a negatively charged protein, showed a faster release rate. The simple method of microgel fabrication, besides the sustained release of the encapsulated proteins, makes the HA microgels a promising vehicle for delivery of cationic proteins.",
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Jooybar, E, Abdekhodaie, MJ & Dijkstra, PJ 2018, Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release. in 2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018., 8703588, 2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018, IEEE, Piscataway, NJ, 2018 25th National and 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018, Qom, Iran, Islamic Republic of, 29/11/18. https://doi.org/10.1109/ICBME.2018.8703588

Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release. / Jooybar, Elaheh; Abdekhodaie, Mohammad J.; Dijkstra, Pieter J.

2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018. Piscataway, NJ : IEEE, 2018. 8703588 (2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release

AU - Jooybar, Elaheh

AU - Abdekhodaie, Mohammad J.

AU - Dijkstra, Pieter J.

PY - 2018/7/2

Y1 - 2018/7/2

N2 - Microgels are hydrophilic polymer matrix with high water content suitable for encapsulation and delivery of biomolecules. In this study, hyaluronic acid (HA) microgels were synthesized using a water in oil emulsion method. First, hyaluronic acid was modified with tyramine, and then the microemulsion was produced by homogenizing the polymer solution in isooctane as an oil phase. HA microdroplets were crosslinked via enzymatic method by addition of horseradish peroxidase (enzyme) and hydrogen peroxide, and stable microgels were produced in a mild crosslinking reaction. According to the results, larger microgels were achieved by increasing the initial polymer concentration. Two sample proteins, Bovine serum albumin (BSA) and lysozyme, were incorporated in the polymer network to investigate the encapsulation efficiency of the microgels. The results demonstrated that the proposed method has a high efficiency for protein encapsulation (> 70%). The release profiles showed that lysozyme, as a cationic protein, was released in a sustained manner over a period of two weeks. However, BSA, as a negatively charged protein, showed a faster release rate. The simple method of microgel fabrication, besides the sustained release of the encapsulated proteins, makes the HA microgels a promising vehicle for delivery of cationic proteins.

AB - Microgels are hydrophilic polymer matrix with high water content suitable for encapsulation and delivery of biomolecules. In this study, hyaluronic acid (HA) microgels were synthesized using a water in oil emulsion method. First, hyaluronic acid was modified with tyramine, and then the microemulsion was produced by homogenizing the polymer solution in isooctane as an oil phase. HA microdroplets were crosslinked via enzymatic method by addition of horseradish peroxidase (enzyme) and hydrogen peroxide, and stable microgels were produced in a mild crosslinking reaction. According to the results, larger microgels were achieved by increasing the initial polymer concentration. Two sample proteins, Bovine serum albumin (BSA) and lysozyme, were incorporated in the polymer network to investigate the encapsulation efficiency of the microgels. The results demonstrated that the proposed method has a high efficiency for protein encapsulation (> 70%). The release profiles showed that lysozyme, as a cationic protein, was released in a sustained manner over a period of two weeks. However, BSA, as a negatively charged protein, showed a faster release rate. The simple method of microgel fabrication, besides the sustained release of the encapsulated proteins, makes the HA microgels a promising vehicle for delivery of cationic proteins.

KW - Enzymatic crosslinking

KW - Hyaluronic acid

KW - Inverse microemulsion

KW - Microgels

KW - Protein delivery

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U2 - 10.1109/ICBME.2018.8703588

DO - 10.1109/ICBME.2018.8703588

M3 - Conference contribution

T3 - 2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018

BT - 2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018

PB - IEEE

CY - Piscataway, NJ

ER -

Jooybar E, Abdekhodaie MJ, Dijkstra PJ. Synthesis of Hyaluronic acid-Tyramine Microgels for Sustained Protein Release. In 2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018. Piscataway, NJ: IEEE. 2018. 8703588. (2018 25th Iranian Conference on Biomedical Engineering and 2018 3rd International Iranian Conference on Biomedical Engineering, ICBME 2018). https://doi.org/10.1109/ICBME.2018.8703588