TY - JOUR
T1 - Tailored Multivalent Targeting of Siglecs with Photosensitizing Liposome Nanocarriers
AU - Almeida-Marrero, Verónica
AU - Bethlehem, Fleur
AU - Longo, Sara
AU - Bertolino, M. Candelaria
AU - Torres, Tomás
AU - Huskens, Jurriaan
AU - de la Escosura, Andrés
N1 - Funding Information:
This work was supported by MINECO-Feder funds [PID2020-116490GB-I00, Porphyrinoids (T.T.) and PID2020-119306GB-100 (AdlE)]. IMDEA Nanociencia also acknowledges support from the “Severo Ochoa” Programme for Centres of Excellence in R&D (MINECO, Grant SEV-2016-0686). S.L. and M.C.B. acknowledge financial support by The Netherlands Organization for Scientific Research (NWO, TOP grant 715.015.001 to J.H.).
Funding Information:
This work was supported by MINECO‐Feder funds [PID2020‐116490GB‐I00, Porphyrinoids (T.T.) and PID2020‐119306GB‐100 (AdlE)]. IMDEA Nanociencia also acknowledges support from the “Severo Ochoa” Programme for Centres of Excellence in R&D (MINECO, Grant SEV‐2016‐0686). S.L. and M.C.B. acknowledge financial support by The Netherlands Organization for Scientific Research (NWO, TOP grant 715.015.001 to J.H.).
Publisher Copyright:
© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
PY - 2022/8/1
Y1 - 2022/8/1
N2 - The modification of surfaces with multiple ligands allows the formation of platforms for the study of multivalency in diverse processes. Herein we use this approach for the implementation of a photosensitizer (PS)-nanocarrier system that binds efficiently to siglec-10, a member of the CD33 family of siglecs (sialic acid (SA)-binding immunoglobulin-like lectins). In particular, a zinc phthalocyanine derivative bearing three SA moieties (PcSA) has been incorporated in the membrane of small unilamellar vesicles (SUVs), retaining its photophysical properties upon insertion into the SUV's membrane. The interaction of these biohybrid systems with human siglec-10-displaying supported lipid bilayers (SLBs) has shown the occurrence of weakly multivalent, superselective interactions between vesicle and SLB. The SLB therefore acts as an excellent cell membrane mimic, while the binding with PS-loaded SUVs shows the potential for targeting siglec-expressing cells with photosensitizing nanocarriers.
AB - The modification of surfaces with multiple ligands allows the formation of platforms for the study of multivalency in diverse processes. Herein we use this approach for the implementation of a photosensitizer (PS)-nanocarrier system that binds efficiently to siglec-10, a member of the CD33 family of siglecs (sialic acid (SA)-binding immunoglobulin-like lectins). In particular, a zinc phthalocyanine derivative bearing three SA moieties (PcSA) has been incorporated in the membrane of small unilamellar vesicles (SUVs), retaining its photophysical properties upon insertion into the SUV's membrane. The interaction of these biohybrid systems with human siglec-10-displaying supported lipid bilayers (SLBs) has shown the occurrence of weakly multivalent, superselective interactions between vesicle and SLB. The SLB therefore acts as an excellent cell membrane mimic, while the binding with PS-loaded SUVs shows the potential for targeting siglec-expressing cells with photosensitizing nanocarriers.
KW - Liposome Nanocarriers
KW - Multivalency
KW - Photosensitizer
KW - Siglecs
UR - http://www.scopus.com/inward/record.url?scp=85132571430&partnerID=8YFLogxK
U2 - 10.1002/anie.202206900
DO - 10.1002/anie.202206900
M3 - Article
C2 - 35652453
AN - SCOPUS:85132571430
SN - 1433-7851
VL - 61
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 31
M1 - e202206900
ER -