Tailoring pulses for coherent raman microscopy

A.C.W. van Rhijn

Research output: ThesisPhD Thesis - Research UT, graduation UT

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This thesis describes improvements of chemical selectivity and contrast in coherent Raman scattering (CRS) spectroscopy and microscopy. Stimulated Raman Scattering (SRS) and Coherent Anti-Stokes Raman Scattering (CARS) provide chemical information of a sample bsed on the vibrational resonances in molecules. These resonances occur at characteristic frequencies related to the mass of the atoms and the strength of the bonds between the atoms. From the presence of these resonances the presence of compounds of interest can be inferred. In conventional narrowband CRS, the contrast is based on a narrow frequency range, covering only a single vibrational resonance. In samples with many compounds, vibrational resonances can overlap significantly and contrast based on a single resonance may not be sufficient to separate different compounds. Furthermore, CARS suffers from a non-resonant background that is generated even in the absence of vibrational resonances. This non-resonant background can overwhelm the resonant CARS signal. The CRS techniques described in this thesis excite multiple vibrational resonances simultaneously. Spectral phase shaping of the excitation light is used to influence the interferences between the different resonances and excitation pathways. In this way selective and background-free images are obtained.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Twente
  • Herek, Jennifer, Supervisor
  • Offerhaus, Herman, Advisor
Award date20 Jul 2012
Place of PublicationEnschede
Print ISBNs978-90-365-3390-4
Publication statusPublished - 20 Jul 2012


  • Technological sciences
  • Optics
  • Physics


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