Tailoring pulses for coherent raman microscopy

A.C.W. van Rhijn

Research output: ThesisPhD Thesis - Research UT, graduation UT

Abstract

This thesis describes improvements of chemical selectivity and contrast in coherent Raman scattering (CRS) spectroscopy and microscopy. Stimulated Raman Scattering (SRS) and Coherent Anti-Stokes Raman Scattering (CARS) provide chemical information of a sample bsed on the vibrational resonances in molecules. These resonances occur at characteristic frequencies related to the mass of the atoms and the strength of the bonds between the atoms. From the presence of these resonances the presence of compounds of interest can be inferred. In conventional narrowband CRS, the contrast is based on a narrow frequency range, covering only a single vibrational resonance. In samples with many compounds, vibrational resonances can overlap significantly and contrast based on a single resonance may not be sufficient to separate different compounds. Furthermore, CARS suffers from a non-resonant background that is generated even in the absence of vibrational resonances. This non-resonant background can overwhelm the resonant CARS signal. The CRS techniques described in this thesis excite multiple vibrational resonances simultaneously. Spectral phase shaping of the excitation light is used to influence the interferences between the different resonances and excitation pathways. In this way selective and background-free images are obtained.
LanguageEnglish
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Herek, Jennifer Lynn, Supervisor
  • Offerhaus, Herman L., Advisor
Award date20 Jul 2012
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-3390-4
DOIs
StatePublished - 20 Jul 2012

Fingerprint

microscopy
Raman spectra
pulses
coherent scattering
theses
excitation
atoms
narrowband
coverings
selectivity
frequency ranges
interference
spectroscopy
molecules

Keywords

  • IR-80832
  • METIS-287032
  • PhysicsTechnological sciencesOptics (see also 3311)Overig onderzoek in de civiele sectorProduktie van instrumenten

Cite this

van Rhijn, A. C. W. (2012). Tailoring pulses for coherent raman microscopy Enschede: Universiteit Twente DOI: 10.3990/1.9789036533904
van Rhijn, A.C.W.. / Tailoring pulses for coherent raman microscopy. Enschede : Universiteit Twente, 2012. 148 p.
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title = "Tailoring pulses for coherent raman microscopy",
abstract = "This thesis describes improvements of chemical selectivity and contrast in coherent Raman scattering (CRS) spectroscopy and microscopy. Stimulated Raman Scattering (SRS) and Coherent Anti-Stokes Raman Scattering (CARS) provide chemical information of a sample bsed on the vibrational resonances in molecules. These resonances occur at characteristic frequencies related to the mass of the atoms and the strength of the bonds between the atoms. From the presence of these resonances the presence of compounds of interest can be inferred. In conventional narrowband CRS, the contrast is based on a narrow frequency range, covering only a single vibrational resonance. In samples with many compounds, vibrational resonances can overlap significantly and contrast based on a single resonance may not be sufficient to separate different compounds. Furthermore, CARS suffers from a non-resonant background that is generated even in the absence of vibrational resonances. This non-resonant background can overwhelm the resonant CARS signal. The CRS techniques described in this thesis excite multiple vibrational resonances simultaneously. Spectral phase shaping of the excitation light is used to influence the interferences between the different resonances and excitation pathways. In this way selective and background-free images are obtained.",
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van Rhijn, ACW 2012, 'Tailoring pulses for coherent raman microscopy', University of Twente, Enschede. DOI: 10.3990/1.9789036533904

Tailoring pulses for coherent raman microscopy. / van Rhijn, A.C.W.

Enschede : Universiteit Twente, 2012. 148 p.

Research output: ThesisPhD Thesis - Research UT, graduation UT

TY - THES

T1 - Tailoring pulses for coherent raman microscopy

AU - van Rhijn,A.C.W.

PY - 2012/7/20

Y1 - 2012/7/20

N2 - This thesis describes improvements of chemical selectivity and contrast in coherent Raman scattering (CRS) spectroscopy and microscopy. Stimulated Raman Scattering (SRS) and Coherent Anti-Stokes Raman Scattering (CARS) provide chemical information of a sample bsed on the vibrational resonances in molecules. These resonances occur at characteristic frequencies related to the mass of the atoms and the strength of the bonds between the atoms. From the presence of these resonances the presence of compounds of interest can be inferred. In conventional narrowband CRS, the contrast is based on a narrow frequency range, covering only a single vibrational resonance. In samples with many compounds, vibrational resonances can overlap significantly and contrast based on a single resonance may not be sufficient to separate different compounds. Furthermore, CARS suffers from a non-resonant background that is generated even in the absence of vibrational resonances. This non-resonant background can overwhelm the resonant CARS signal. The CRS techniques described in this thesis excite multiple vibrational resonances simultaneously. Spectral phase shaping of the excitation light is used to influence the interferences between the different resonances and excitation pathways. In this way selective and background-free images are obtained.

AB - This thesis describes improvements of chemical selectivity and contrast in coherent Raman scattering (CRS) spectroscopy and microscopy. Stimulated Raman Scattering (SRS) and Coherent Anti-Stokes Raman Scattering (CARS) provide chemical information of a sample bsed on the vibrational resonances in molecules. These resonances occur at characteristic frequencies related to the mass of the atoms and the strength of the bonds between the atoms. From the presence of these resonances the presence of compounds of interest can be inferred. In conventional narrowband CRS, the contrast is based on a narrow frequency range, covering only a single vibrational resonance. In samples with many compounds, vibrational resonances can overlap significantly and contrast based on a single resonance may not be sufficient to separate different compounds. Furthermore, CARS suffers from a non-resonant background that is generated even in the absence of vibrational resonances. This non-resonant background can overwhelm the resonant CARS signal. The CRS techniques described in this thesis excite multiple vibrational resonances simultaneously. Spectral phase shaping of the excitation light is used to influence the interferences between the different resonances and excitation pathways. In this way selective and background-free images are obtained.

KW - IR-80832

KW - METIS-287032

KW - PhysicsTechnological sciencesOptics (see also 3311)Overig onderzoek in de civiele sectorProduktie van instrumenten

U2 - 10.3990/1.9789036533904

DO - 10.3990/1.9789036533904

M3 - PhD Thesis - Research UT, graduation UT

SN - 978-90-365-3390-4

PB - Universiteit Twente

CY - Enschede

ER -

van Rhijn ACW. Tailoring pulses for coherent raman microscopy. Enschede: Universiteit Twente, 2012. 148 p. Available from, DOI: 10.3990/1.9789036533904