TY - JOUR
T1 - Tailoring the stealth properties of biocompatible polysaccharide nanocontainers
AU - Kang, Biao
AU - Okwieka, Patricia
AU - Schöttler, Susanne
AU - Seifert, Oliver
AU - Kontermann, Roland E.
AU - Pfizenmaier, Klaus
AU - Musyanovych, Anna
AU - Meyer, Ralf
AU - Diken, Mustafa
AU - Sahin, Ugur
AU - Mailänder, Volker
AU - Wurm, Frederik R.
AU - Landfester, Katharina
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Fundamental development of a biocompatible and degradable nanocarrier platform based on hydroxyethyl starch (HES) is reported. HES is a derivative of starch and possesses both high biocompatibility and improved stability against enzymatic degradation; it is used to prepare nanocapsules via the polyaddition reaction at the interface of water nanodroplets dispersed in an organic miniemulsion. The synthesized hollow nanocapsules can be loaded with hydrophilic guests in its aqueous core, tuned in size, chemically functionalized in various pathways, and show high shelf life stability. The surface of the HES nanocapsules is further functionalized with poly(ethylene glycol) via different chemistries, which substantially enhanced blood half-life time. Importantly, methods for precise and reliable quantification of the degree of functionalization are also introduced, which enable the precise control of the chemistry on the capsules' surface. The stealth properties of these capsules is studied both in-vitro and in-vivo. The functionalized nanocapsules serve as a modular platform for specific cell targeting, as they show no unspecific up-taken by different cell types and show very long circulating time in blood (up to 72h).
AB - Fundamental development of a biocompatible and degradable nanocarrier platform based on hydroxyethyl starch (HES) is reported. HES is a derivative of starch and possesses both high biocompatibility and improved stability against enzymatic degradation; it is used to prepare nanocapsules via the polyaddition reaction at the interface of water nanodroplets dispersed in an organic miniemulsion. The synthesized hollow nanocapsules can be loaded with hydrophilic guests in its aqueous core, tuned in size, chemically functionalized in various pathways, and show high shelf life stability. The surface of the HES nanocapsules is further functionalized with poly(ethylene glycol) via different chemistries, which substantially enhanced blood half-life time. Importantly, methods for precise and reliable quantification of the degree of functionalization are also introduced, which enable the precise control of the chemistry on the capsules' surface. The stealth properties of these capsules is studied both in-vitro and in-vivo. The functionalized nanocapsules serve as a modular platform for specific cell targeting, as they show no unspecific up-taken by different cell types and show very long circulating time in blood (up to 72h).
KW - Hydroxyl ethyl starch
KW - Miniemulsion
KW - Nanocarrier
KW - PEGylation
KW - Stealth effect
UR - http://www.scopus.com/inward/record.url?scp=84924517839&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2015.01.042
DO - 10.1016/j.biomaterials.2015.01.042
M3 - Article
C2 - 25725561
AN - SCOPUS:84924517839
VL - 49
SP - 125
EP - 134
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
ER -