TGF-? and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro

Ilse M.S.L. Khouw, Pauline B. van Wachem, Josée A. Plantinga, Zeljko Vujaskovic, M.J.B. Wissink, Lou F.M.H. de Leij, Marja J.A. van Luyn

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Abstract

Fibroblasts and myofibroblasts are involved in the foreign body reaction to biomaterials, especially in capsule formation. However, contraction or detachment of the capsule can lead to complications. Biocompatibility of biomaterials may be improved by the application of proteins regulating the differentiation or activation of (myo)fibroblasts. Myofibroblasts, differentiating from fibroblasts can be identified by the expression of α-smooth muscle actin (α-SM actin). We investigated the influence of proliferation and quiescence on the differentiation of porcine dermal cells and whether transforming growth factor-β (TGF-β) and basic fibroblast growth factor (bFGF) are involved in the differentiation of proliferating cells. Porcine cells were used because pigs increasingly function as in vivo models while little is known of the characteristics of their cells. Serum-free cultured, quiescent fibroblasts differentiated into myofibroblasts, while proliferating fibroblasts cultured in the presence of serum containing TGF-β, formed α-SM actin-negative cell clusters. After reaching confluency, these clusters started to expressing α-SM actin. Moreover, these proliferating cells produced TGF-β from day 4 onwards while bFGF did not. Differentiation into myofibroblasts was inhibited by bFGF and to an even greater extent by antibodies to TGF-β. Further, two theories concerning the role of the myofibroblast in tissue contraction in view of two biomaterial application will be discussed.
Original languageUndefined
Pages (from-to)1815-1822
JournalBiomaterials
Volume20
Issue number19
DOIs
Publication statusPublished - 1999

Keywords

  • METIS-105670
  • IR-74179
  • Differentiation
  • bFGF
  • Porcine (myo)fibroblast
  • TGF-β
  • Proliferation

Cite this

Khouw, I. M. S. L., van Wachem, P. B., Plantinga, J. A., Vujaskovic, Z., Wissink, M. J. B., de Leij, L. F. M. H., & van Luyn, M. J. A. (1999). TGF-? and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro. Biomaterials, 20(19), 1815-1822. https://doi.org/10.1016/S0142-9612(99)00077-0