The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface

J.A. Ronner; H.G.W. Lensen; P.M.F.G. Olthuis; C.A. Smolders; J. Feijen

doi:10.1016/0142-9612(84)90023-1

The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface

J.A. Ronner, H.G.W. Lensen, P.M.F.G. Olthuis, C.A. Smolders, J. Feijen

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Abstract

The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly, one preparation showed a plateau value of 0.4 μg cm−2 which was reached at 0.1g I−1, whereas the other preparation showed no plateau value within the concentration range studied (0.1–7.0 g I−1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out These results stress the necessity for proper characterization of proteins used in adsorption studies.

Original language	English
Pages (from-to)	241
Journal	Biomaterials
Volume	5
Issue number	4
DOIs	https://doi.org/10.1016/0142-9612(84)90023-1
Publication status	Published - 1984

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title = "The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface",

abstract = "The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly, one preparation showed a plateau value of 0.4 μg cm−2 which was reached at 0.1g I−1, whereas the other preparation showed no plateau value within the concentration range studied (0.1–7.0 g I−1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out These results stress the necessity for proper characterization of proteins used in adsorption studies.",

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T1 - The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface

AU - Ronner, J.A.

AU - Lensen, H.G.W.

AU - Olthuis, P.M.F.G.

AU - Smolders, C.A.

AU - Feijen, J.

PY - 1984

Y1 - 1984

N2 - The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly, one preparation showed a plateau value of 0.4 μg cm−2 which was reached at 0.1g I−1, whereas the other preparation showed no plateau value within the concentration range studied (0.1–7.0 g I−1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out These results stress the necessity for proper characterization of proteins used in adsorption studies.

AB - The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly, one preparation showed a plateau value of 0.4 μg cm−2 which was reached at 0.1g I−1, whereas the other preparation showed no plateau value within the concentration range studied (0.1–7.0 g I−1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out These results stress the necessity for proper characterization of proteins used in adsorption studies.

U2 - 10.1016/0142-9612(84)90023-1

DO - 10.1016/0142-9612(84)90023-1

M3 - Article

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VL - 5

SP - 241

JO - Biomaterials

JF - Biomaterials

IS - 4

ER -