The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly, one preparation showed a plateau value of 0.4 μg cm−2 which was reached at 0.1g I−1, whereas the other preparation showed no plateau value within the concentration range studied (0.1–7.0 g I−1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out These results stress the necessity for proper characterization of proteins used in adsorption studies.
Ronner, J. A., Lensen, H. G. W., Olthuis, P. M. F. G., Smolders, C. A., & Feijen, J. (1984). The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface. Biomaterials, 5(4), 241. https://doi.org/10.1016/0142-9612(84)90023-1