The adsorption behaviour of two commercial IgG-preparations onto a polystyrene latex surface

J.A. Ronner, H.G.W. Lensen, P.M.F.G. Olthuis, C.A. Smolders, J. Feijen

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The adsorption behaviour of two commercial preparations of human IgG onto a polystyrene latex surface was studied. The adsorption isotherms obtained differed markedly, one preparation showed a plateau value of 0.4 μg cm−2 which was reached at 0.1g I−1, whereas the other preparation showed no plateau value within the concentration range studied (0.1–7.0 g I−1). Characterization by means of iso-electric focusing and HPLC also showed differences between the two preparations. No differences were observed when immuno-electrophoresis was carried out These results stress the necessity for proper characterization of proteins used in adsorption studies.
Original languageEnglish
Pages (from-to)241
Issue number4
Publication statusPublished - 1984

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