The critical sensor: a new type of evanescent wave immunosensor

E.F. Schipper, R.P.H. Kooyman, A. Borreman, J. Greve

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)
71 Downloads (Pure)

Abstract

A new planar waveguide immunosensor has been developed in which adsorption at a surface, changing the refractive index contrast, is measured. In this ¿critical¿ sensor the change in the effective refractive index contrast is transducted to a shift of the critical reflection angle. The sensor's response after a specific binding of antigens to antibodies is discussed theoretically. In addition, an experimental sensitivity evaluation on the basis of several immunosensing experiments is presented. The obtained lower detection limit is 2 × 10¿2 nm in adlayer growth, equivalent to 12 pg/mm2 of analyte coverage. This sensitivity is comparable to the performance of the surface plasmon resonance sensors or the grating coupler sensors. However, the ¿critical¿ sensor has some advantages. These are mainly the ease of fabrication and adjustment prior to a measurement, and the fact for an experiment no metal layer has to be used.
Original languageEnglish
Pages (from-to)295-304
Number of pages10
JournalBiosensors and bioelectronics
Volume11
Issue number3
DOIs
Publication statusPublished - 1996

Keywords

  • Immunosensor
  • Planar waveguides
  • Deflection
  • Critical angle
  • Evanescent wave sensor

Fingerprint Dive into the research topics of 'The critical sensor: a new type of evanescent wave immunosensor'. Together they form a unique fingerprint.

  • Cite this