Blood perfusion assays are a good way to determine the state of an endothelial layer in a blood vessel-on-chip model. Multiple research projects have shown the advantages of using blood perfusion assays in blood vessel-on-chip models. These models are usually based on square microchannels that do not represent the in vivo geometries of a real blood vessel.
Using induced pluripotent stem cell derived endothelial cells and polarized THP-1 monocytes, we have developed a 3D blood vessel-on-chip with embedded macrophages, which is created using sequential cell seeding in viscous finger-patterned collagen hydrogels. We have set up a human whole blood perfusion assay for these 3D blood vessel-chips.
Platelet formation was seen in brightfield images, but were not observed in fixated samples, due to washing steps to clear the channels of residual blood. An increased formation of fibrin in the blood vessel-on-chip models containing (lipid loaden) macrophages was observed indicating an increasing in the blood clotting process in these channels.
In conclusion, we have developed a 3D vessel-on-chip model with embedded macrophages and have demonstrated that it can be perfused with human whole blood. With the first version of the 3D model established, we expect to use it in future studies of the role of macrophages in thromboinflammation.
|Title of host publication
|Abstracts of the 2nd Microphysiological Systems World Summit, Berlin, 2023
|Published - 26 Jun 2023
|2nd Microphysiological Systems World Summit, 2023 - Berlin, Germany
Duration: 26 Jun 2023 → 30 Jun 2023
Conference number: 2
|2nd Microphysiological Systems World Summit, 2023
|26/06/23 → 30/06/23