The effect of PEGT/PBT scaffold architecture on the composition of tissue engineered cartilage

J. Malda, T.B.F. Woodfield, F. van der Vloodt, C. Wilson, C.E. Wilson, D.E. Martens, J. Tramper, Clemens van Blitterswijk, J.U. Riesle

Research output: Contribution to journalArticleAcademicpeer-review

189 Citations (Scopus)

Abstract

A highly interconnecting and accessible pore network has been suggested as one of a number of prerequisites in the design of scaffolds for tissue engineering. In the present study, two processing techniques, compression-molding/particulate-leaching (CM), and 3D fiber deposition (3DF), were used to develop porous scaffolds from biodegradable poly(ethylene glycol)-terephthalate/poly(butylene terephthalate) (PEGT/PBT) co-polymers with varying pore architectures. Three-dimensional micro-computed tomography (μCT) was used to characterize scaffold architectures and scaffolds were seeded with articular chondrocytes to evaluate tissue formation. Scaffold porosity ranged between 75% and 80%. Average pore size of tortuous CM scaffolds (182 μm) was lower than those of organized 3DF scaffolds (525 μm). The weight ratio of glycosaminoglycans (GAG)/DNA, as a measure of cartilage-like tissue formation, did not change after 14 days of culture whereas, following subcutaneous implantation, GAG/DNA increased significantly and was significantly higher in 3DF constructs than in CM constructs, whilst collagen type II was present within both constructs. In conclusion, 3DF PEGT/PBT scaffolds create an environment in vivo that enhances cartilaginous matrix deposition and hold particular promise for treatment of articular cartilage defects.
Original languageUndefined
Pages (from-to)63-72
JournalBiomaterials
Volume26
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • In vitro
  • In vivo
  • Scaffold
  • Chondrocytes
  • IR-67209
  • Cell culture
  • Cartilage tissue engineering
  • METIS-223535

Cite this