The Extraction and Recovery Efficiency of Pure DNA for Different Types of Swabs

Brigitte Bibiche Bruijns; Roald M. Tiggelaar; H. Gardeniers

doi:10.1111/1556-4029.13837

The Extraction and Recovery Efficiency of Pure DNA for Different Types of Swabs

Brigitte Bibiche Bruijns (Corresponding Author), Roald M. Tiggelaar, H. Gardeniers

Mesoscale Chemical Systems

Research output: Contribution to journal › Article › Academic › peer-review

80 Citations (Scopus)

621 Downloads (Pure)

Abstract

The extraction and recovery efficiency of swabs used to collect evidence at crime scenes is relatively low (typically <50%) for bacterial spores and body fluids. Cell‐free deoxyribonucleic acid (DNA) is an interesting alternative compared to whole cells as a source for forensic analysis, but extraction and recovery from swabs has not been tested before using pure DNA. In this study cotton, foam, nylon flocked, polyester and rayon swabs are investigated in order to collect pure DNA isolated from saliva samples. The morphology and absorption capacity of swabs is studied. Extraction and recovery efficiencies are determined and compared to the maximum theoretical efficiency. The results indicate that a substantial part of DNA is not extracted from the swab and some types of swab seem to bind effectively with DNA. The efficiency of the different types of swab never exceeds 50%. The nylon flocked 4N6FLOQSwab used for buccal sampling performs the best.

Original language	English
Pages (from-to)	1492-1499
Number of pages	8
Journal	Journal of Forensic Sciences
Volume	63
Issue number	5
Early online date	11 Jun 2018
DOIs	https://doi.org/10.1111/1556-4029.13837
Publication status	Published - 1 Sept 2018

Keywords

UT-Hybrid-D

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10.1111/1556-4029.13837Licence: CC BY

Bruijns_et_al-2018-Journal_of_Forensic_SciencesFinal published version, 281 KBLicence: CC BY-NC

80 Citations
1 PhD Thesis - Research external, graduation UT

Microfluidic devices for presumptive forensic tests
Bruijns, B. B., 18 Jan 2019, Enschede: University of Twente. 340 p.
Research output: Thesis › PhD Thesis - Research external, graduation UT

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abstract = "The extraction and recovery efficiency of swabs used to collect evidence at crime scenes is relatively low (typically <50%) for bacterial spores and body fluids. Cell‐free deoxyribonucleic acid (DNA) is an interesting alternative compared to whole cells as a source for forensic analysis, but extraction and recovery from swabs has not been tested before using pure DNA. In this study cotton, foam, nylon flocked, polyester and rayon swabs are investigated in order to collect pure DNA isolated from saliva samples. The morphology and absorption capacity of swabs is studied. Extraction and recovery efficiencies are determined and compared to the maximum theoretical efficiency. The results indicate that a substantial part of DNA is not extracted from the swab and some types of swab seem to bind effectively with DNA. The efficiency of the different types of swab never exceeds 50%. The nylon flocked 4N6FLOQSwab used for buccal sampling performs the best.",

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The Extraction and Recovery Efficiency of Pure DNA for Different Types of Swabs

Abstract

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Research output

Microfluidic devices for presumptive forensic tests

Cite this