The influence of nanoscale grooved substrates on osteoblast behavior and extracellular matrix depostion

Edwin Lamers, X. Frank Walboomers, Maciej Domanski, Joost te Riet, Falco C.M.J.M. van Delft, Regina Lüttge, Louis A.J.A. Winnubst, Han J.G.E. Gardeniers, John A. Jansen

Research output: Contribution to journalArticleAcademicpeer-review

161 Citations (Scopus)

Abstract

To fight bone diseases characterized by poor bone quality like osteoporosis and osteoarthritis, as well as in reconstructive surgery, there is a need for a new generation of implantable biomaterials. It is envisioned that implant surfaces can be improved by mimicking the natural extracellular matrix of bone tissue, which is highly a organized nano-composite. In this study we aimed to get a better understanding of osteoblast response to nanometric grooved substrates varying in height, width and spacing. A throughput screening biochip was created using electron beam lithography. Subsequently, uniform large-scale nanogrooved substrates were created using laser interference lithography and reactive ion etching. Results showed that osteoblasts were responsive to nanopatterns down to 75 nm in width and 33 nm in depth. SEM and TEM studies showed that an osteoblast-driven calcium phosphate (CaP) mineralization was observed to follow the surface pattern dimensions. Strikingly, aligned mineralization was found on even smaller nanopatterns of 50 nm in width and 17 nm in depth. A single cell based approach for real time PCR demonstrated that osteoblast-specific gene expression was increased on nanopatterns relative to a smooth control. The results indicate that nanogrooves can be a very promising tool to direct the bone response at the interface between an implant and the bone tissue.
Original languageEnglish
Pages (from-to)3307-3316
Number of pages10
JournalBiomaterials
Volume31
Issue number12
DOIs
Publication statusPublished - 2010

Fingerprint

Osteoblasts
Extracellular Matrix
Bone
Bone and Bones
Substrates
Reconstructive Surgical Procedures
Bone Diseases
Tissue
Biocompatible Materials
Biochips
Osteoarthritis
Electron beam lithography
Osteoporosis
Reactive ion etching
Calcium phosphate
Real-Time Polymerase Chain Reaction
Lasers
Gene expression
Biomaterials
Surgery

Keywords

  • Interface
  • Calcification
  • Cell morphology
  • Nanotopography
  • Osteoblast
  • Gene expression

Cite this

Lamers, E., Walboomers, X. F., Domanski, M., te Riet, J., van Delft, F. C. M. J. M., Lüttge, R., ... Jansen, J. A. (2010). The influence of nanoscale grooved substrates on osteoblast behavior and extracellular matrix depostion. Biomaterials, 31(12), 3307-3316. https://doi.org/10.1016/j.biomaterials.2010.01.034
Lamers, Edwin ; Walboomers, X. Frank ; Domanski, Maciej ; te Riet, Joost ; van Delft, Falco C.M.J.M. ; Lüttge, Regina ; Winnubst, Louis A.J.A. ; Gardeniers, Han J.G.E. ; Jansen, John A. / The influence of nanoscale grooved substrates on osteoblast behavior and extracellular matrix depostion. In: Biomaterials. 2010 ; Vol. 31, No. 12. pp. 3307-3316.
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Lamers, E, Walboomers, XF, Domanski, M, te Riet, J, van Delft, FCMJM, Lüttge, R, Winnubst, LAJA, Gardeniers, HJGE & Jansen, JA 2010, 'The influence of nanoscale grooved substrates on osteoblast behavior and extracellular matrix depostion' Biomaterials, vol. 31, no. 12, pp. 3307-3316. https://doi.org/10.1016/j.biomaterials.2010.01.034

The influence of nanoscale grooved substrates on osteoblast behavior and extracellular matrix depostion. / Lamers, Edwin; Walboomers, X. Frank; Domanski, Maciej; te Riet, Joost; van Delft, Falco C.M.J.M.; Lüttge, Regina; Winnubst, Louis A.J.A.; Gardeniers, Han J.G.E.; Jansen, John A.

In: Biomaterials, Vol. 31, No. 12, 2010, p. 3307-3316.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The influence of nanoscale grooved substrates on osteoblast behavior and extracellular matrix depostion

AU - Lamers, Edwin

AU - Walboomers, X. Frank

AU - Domanski, Maciej

AU - te Riet, Joost

AU - van Delft, Falco C.M.J.M.

AU - Lüttge, Regina

AU - Winnubst, Louis A.J.A.

AU - Gardeniers, Han J.G.E.

AU - Jansen, John A.

PY - 2010

Y1 - 2010

N2 - To fight bone diseases characterized by poor bone quality like osteoporosis and osteoarthritis, as well as in reconstructive surgery, there is a need for a new generation of implantable biomaterials. It is envisioned that implant surfaces can be improved by mimicking the natural extracellular matrix of bone tissue, which is highly a organized nano-composite. In this study we aimed to get a better understanding of osteoblast response to nanometric grooved substrates varying in height, width and spacing. A throughput screening biochip was created using electron beam lithography. Subsequently, uniform large-scale nanogrooved substrates were created using laser interference lithography and reactive ion etching. Results showed that osteoblasts were responsive to nanopatterns down to 75 nm in width and 33 nm in depth. SEM and TEM studies showed that an osteoblast-driven calcium phosphate (CaP) mineralization was observed to follow the surface pattern dimensions. Strikingly, aligned mineralization was found on even smaller nanopatterns of 50 nm in width and 17 nm in depth. A single cell based approach for real time PCR demonstrated that osteoblast-specific gene expression was increased on nanopatterns relative to a smooth control. The results indicate that nanogrooves can be a very promising tool to direct the bone response at the interface between an implant and the bone tissue.

AB - To fight bone diseases characterized by poor bone quality like osteoporosis and osteoarthritis, as well as in reconstructive surgery, there is a need for a new generation of implantable biomaterials. It is envisioned that implant surfaces can be improved by mimicking the natural extracellular matrix of bone tissue, which is highly a organized nano-composite. In this study we aimed to get a better understanding of osteoblast response to nanometric grooved substrates varying in height, width and spacing. A throughput screening biochip was created using electron beam lithography. Subsequently, uniform large-scale nanogrooved substrates were created using laser interference lithography and reactive ion etching. Results showed that osteoblasts were responsive to nanopatterns down to 75 nm in width and 33 nm in depth. SEM and TEM studies showed that an osteoblast-driven calcium phosphate (CaP) mineralization was observed to follow the surface pattern dimensions. Strikingly, aligned mineralization was found on even smaller nanopatterns of 50 nm in width and 17 nm in depth. A single cell based approach for real time PCR demonstrated that osteoblast-specific gene expression was increased on nanopatterns relative to a smooth control. The results indicate that nanogrooves can be a very promising tool to direct the bone response at the interface between an implant and the bone tissue.

KW - Interface

KW - Calcification

KW - Cell morphology

KW - Nanotopography

KW - Osteoblast

KW - Gene expression

U2 - 10.1016/j.biomaterials.2010.01.034

DO - 10.1016/j.biomaterials.2010.01.034

M3 - Article

VL - 31

SP - 3307

EP - 3316

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 12

ER -