The intensity of the 1602 cm-1 band in human cells is related to mitochondrial activity

V.V. Pully, Cornelis Otto

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

We report a Raman band at 1602 cm−1 in the spectra of human cells, which previously had only been observed in mitochondria of yeast cells. This band, which has not yet been assigned to a particular molecular species, was found to occur in HeLa cells, peripheral blood lymphocytes, human mesenchymal stem cells and bovine chondrocytes. The band is proposed as an indicator of the activity of mitochondria in cells. Cells were cultured with and without serum or temporarily deprived of serum. The band can be observed for all these variations in cell culture methodology. The band intensity decreases under the influence of an increase of the calcium ion concentration in the surrounding medium.
Original languageUndefined
Pages (from-to)473-475
Number of pages3
JournalJournal of raman spectroscopy
Volume40
Issue number5
DOIs
Publication statusPublished - 2009

Keywords

  • Mitochondria
  • IR-72592
  • living human cells
  • Ca2+ influence
  • hyperspectral Raman imaging
  • METIS-258857

Cite this