The long-range organization of a native photosynthetic membrane

Raoul N. Frese, C. Alistair Siebert, Robert A. Niederman, C. Neil Hunter, Cees Otto, Rienk van Grondelle

    Research output: Contribution to journalArticleAcademicpeer-review

    52 Citations (Scopus)
    19 Downloads (Pure)

    Abstract

    Photosynthesis relies on the delicate interplay between a specific set of membrane-bound pigment–protein complexes that harvest and transport solar energy, execute charge separation, and conserve the energy. We have investigated the organization of the light-harvesting (LH) and reaction-center (RC) complexes in native bacterial photosynthetic membranes of the purple bacterium Rhodobacter sphaeroides by using polarized light spectroscopy, linear dichroism (LD) on oriented membranes. These LD measurements show that in native membranes, which contain LH2 as the major energy absorber, the RC–LH1–PufX complexes are highly organized in a way similar to that which we found previously for a mutant lacking LH2. The relative contribution of LH1 and LH2 light-harvesting complexes to the LD spectrum shows that LH2 preferentially resides in highly curved parts of the membrane. Combining the spectroscopic data with our recent atomic force microscopy (AFM) results, we propose an organization for this photosynthetic membrane that features domains containing linear arrays of RC–LH1–PufX complexes interspersed with LH2 complexes and some LH2 found in separate domains. The study described here allows the simultaneous assessment of both global and local structural information on the organization of intact, untreated membranes.
    Original languageEnglish
    Pages (from-to)17994-17999
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume101
    Issue number52
    DOIs
    Publication statusPublished - 28 Dec 2004

    Fingerprint

    Photosynthetic membranes
    Membranes
    Photosynthesis
    Dichroism
    Light polarization
    Solar energy
    Atomic force microscopy
    Spectroscopy

    Cite this

    Frese, Raoul N. ; Siebert, C. Alistair ; Niederman, Robert A. ; Hunter, C. Neil ; Otto, Cees ; van Grondelle, Rienk. / The long-range organization of a native photosynthetic membrane. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 52. pp. 17994-17999.
    @article{68e13c4651bf43a9954202577f823da5,
    title = "The long-range organization of a native photosynthetic membrane",
    abstract = "Photosynthesis relies on the delicate interplay between a specific set of membrane-bound pigment–protein complexes that harvest and transport solar energy, execute charge separation, and conserve the energy. We have investigated the organization of the light-harvesting (LH) and reaction-center (RC) complexes in native bacterial photosynthetic membranes of the purple bacterium Rhodobacter sphaeroides by using polarized light spectroscopy, linear dichroism (LD) on oriented membranes. These LD measurements show that in native membranes, which contain LH2 as the major energy absorber, the RC–LH1–PufX complexes are highly organized in a way similar to that which we found previously for a mutant lacking LH2. The relative contribution of LH1 and LH2 light-harvesting complexes to the LD spectrum shows that LH2 preferentially resides in highly curved parts of the membrane. Combining the spectroscopic data with our recent atomic force microscopy (AFM) results, we propose an organization for this photosynthetic membrane that features domains containing linear arrays of RC–LH1–PufX complexes interspersed with LH2 complexes and some LH2 found in separate domains. The study described here allows the simultaneous assessment of both global and local structural information on the organization of intact, untreated membranes.",
    author = "Frese, {Raoul N.} and Siebert, {C. Alistair} and Niederman, {Robert A.} and Hunter, {C. Neil} and Cees Otto and {van Grondelle}, Rienk",
    year = "2004",
    month = "12",
    day = "28",
    doi = "10.1073/pnas.0407295102",
    language = "English",
    volume = "101",
    pages = "17994--17999",
    journal = "Proceedings of the National Academy of Sciences of the United States of America",
    issn = "0027-8424",
    publisher = "National Academy of Sciences",
    number = "52",

    }

    The long-range organization of a native photosynthetic membrane. / Frese, Raoul N.; Siebert, C. Alistair; Niederman, Robert A.; Hunter, C. Neil; Otto, Cees; van Grondelle, Rienk.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 52, 28.12.2004, p. 17994-17999.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - The long-range organization of a native photosynthetic membrane

    AU - Frese, Raoul N.

    AU - Siebert, C. Alistair

    AU - Niederman, Robert A.

    AU - Hunter, C. Neil

    AU - Otto, Cees

    AU - van Grondelle, Rienk

    PY - 2004/12/28

    Y1 - 2004/12/28

    N2 - Photosynthesis relies on the delicate interplay between a specific set of membrane-bound pigment–protein complexes that harvest and transport solar energy, execute charge separation, and conserve the energy. We have investigated the organization of the light-harvesting (LH) and reaction-center (RC) complexes in native bacterial photosynthetic membranes of the purple bacterium Rhodobacter sphaeroides by using polarized light spectroscopy, linear dichroism (LD) on oriented membranes. These LD measurements show that in native membranes, which contain LH2 as the major energy absorber, the RC–LH1–PufX complexes are highly organized in a way similar to that which we found previously for a mutant lacking LH2. The relative contribution of LH1 and LH2 light-harvesting complexes to the LD spectrum shows that LH2 preferentially resides in highly curved parts of the membrane. Combining the spectroscopic data with our recent atomic force microscopy (AFM) results, we propose an organization for this photosynthetic membrane that features domains containing linear arrays of RC–LH1–PufX complexes interspersed with LH2 complexes and some LH2 found in separate domains. The study described here allows the simultaneous assessment of both global and local structural information on the organization of intact, untreated membranes.

    AB - Photosynthesis relies on the delicate interplay between a specific set of membrane-bound pigment–protein complexes that harvest and transport solar energy, execute charge separation, and conserve the energy. We have investigated the organization of the light-harvesting (LH) and reaction-center (RC) complexes in native bacterial photosynthetic membranes of the purple bacterium Rhodobacter sphaeroides by using polarized light spectroscopy, linear dichroism (LD) on oriented membranes. These LD measurements show that in native membranes, which contain LH2 as the major energy absorber, the RC–LH1–PufX complexes are highly organized in a way similar to that which we found previously for a mutant lacking LH2. The relative contribution of LH1 and LH2 light-harvesting complexes to the LD spectrum shows that LH2 preferentially resides in highly curved parts of the membrane. Combining the spectroscopic data with our recent atomic force microscopy (AFM) results, we propose an organization for this photosynthetic membrane that features domains containing linear arrays of RC–LH1–PufX complexes interspersed with LH2 complexes and some LH2 found in separate domains. The study described here allows the simultaneous assessment of both global and local structural information on the organization of intact, untreated membranes.

    U2 - 10.1073/pnas.0407295102

    DO - 10.1073/pnas.0407295102

    M3 - Article

    VL - 101

    SP - 17994

    EP - 17999

    JO - Proceedings of the National Academy of Sciences of the United States of America

    JF - Proceedings of the National Academy of Sciences of the United States of America

    SN - 0027-8424

    IS - 52

    ER -