The number of α-synuclein proteins per vesicle gives insights into its physiological function

Research output: Contribution to conferencePoster

11 Citations (Scopus)

Abstract

Although it is well established that the protein α-synuclein (αS) plays an important role in Parkinson’s disease, its physiological function remains largely unknown. It has been reported to bind membranes and to play a role in membrane remodeling processes. The mechanism by which αS remodels membranes is still debated; it may either affect its physical properties or act as a chaperone for other membrane associated proteins.
To obtain insight into the role of αS in membrane remodeling we investigated the number of αS proteins associated with single small vesicles in a neuronal cell model. Using single-molecule microscopy and photo-bleaching approaches, we most frequently found 70 αS-GFPs per vesicle. Although this number is high enough to modulate physical membrane properties, it is also strikingly similar to the number of synaptobrevins, a putative interaction partner of αS, per vesicle. We therefore hypothesize a dual synergistic role for αS in membrane remodeling acting both as a modulator of the membrane’s physical properties and interaction partner for synaptobrevin.
Original languageEnglish
Publication statusPublished - 2016
EventDutchBiophysics 2016 - Veldhoven, Netherlands
Duration: 3 Oct 20164 Oct 2016
https://www.nwo-i.nl/wp-content/uploads/2016/04/DUTCHBIO16-Programmaposter-A3_4_DEF.pdf

Conference

ConferenceDutchBiophysics 2016
CountryNetherlands
CityVeldhoven
Period3/10/164/10/16
Internet address

Fingerprint

Synucleins
Membranes
R-SNARE Proteins
Proteins
Protein S
Parkinson Disease
Membrane Proteins

Keywords

  • α-synuclein
  • number
  • vesicles
  • physiological function
  • photo-bleaching

Cite this

@conference{089dbcf79ea141fdb6cf5ff26f155622,
title = "The number of α-synuclein proteins per vesicle gives insights into its physiological function",
abstract = "Although it is well established that the protein α-synuclein (αS) plays an important role in Parkinson’s disease, its physiological function remains largely unknown. It has been reported to bind membranes and to play a role in membrane remodeling processes. The mechanism by which αS remodels membranes is still debated; it may either affect its physical properties or act as a chaperone for other membrane associated proteins.To obtain insight into the role of αS in membrane remodeling we investigated the number of αS proteins associated with single small vesicles in a neuronal cell model. Using single-molecule microscopy and photo-bleaching approaches, we most frequently found 70 αS-GFPs per vesicle. Although this number is high enough to modulate physical membrane properties, it is also strikingly similar to the number of synaptobrevins, a putative interaction partner of αS, per vesicle. We therefore hypothesize a dual synergistic role for αS in membrane remodeling acting both as a modulator of the membrane’s physical properties and interaction partner for synaptobrevin.",
keywords = "α-synuclein , number , vesicles, physiological function, photo-bleaching",
author = "{Abolghassemi Fakhree}, {Mohammad Amin} and Christian Blum and Claessens, {Mireille Maria Anna Elisabeth}",
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language = "English",
note = "DutchBiophysics 2016 ; Conference date: 03-10-2016 Through 04-10-2016",
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The number of α-synuclein proteins per vesicle gives insights into its physiological function. / Abolghassemi Fakhree, Mohammad Amin; Blum, Christian ; Claessens, Mireille Maria Anna Elisabeth.

2016. Poster session presented at DutchBiophysics 2016, Veldhoven, Netherlands.

Research output: Contribution to conferencePoster

TY - CONF

T1 - The number of α-synuclein proteins per vesicle gives insights into its physiological function

AU - Abolghassemi Fakhree, Mohammad Amin

AU - Blum, Christian

AU - Claessens, Mireille Maria Anna Elisabeth

PY - 2016

Y1 - 2016

N2 - Although it is well established that the protein α-synuclein (αS) plays an important role in Parkinson’s disease, its physiological function remains largely unknown. It has been reported to bind membranes and to play a role in membrane remodeling processes. The mechanism by which αS remodels membranes is still debated; it may either affect its physical properties or act as a chaperone for other membrane associated proteins.To obtain insight into the role of αS in membrane remodeling we investigated the number of αS proteins associated with single small vesicles in a neuronal cell model. Using single-molecule microscopy and photo-bleaching approaches, we most frequently found 70 αS-GFPs per vesicle. Although this number is high enough to modulate physical membrane properties, it is also strikingly similar to the number of synaptobrevins, a putative interaction partner of αS, per vesicle. We therefore hypothesize a dual synergistic role for αS in membrane remodeling acting both as a modulator of the membrane’s physical properties and interaction partner for synaptobrevin.

AB - Although it is well established that the protein α-synuclein (αS) plays an important role in Parkinson’s disease, its physiological function remains largely unknown. It has been reported to bind membranes and to play a role in membrane remodeling processes. The mechanism by which αS remodels membranes is still debated; it may either affect its physical properties or act as a chaperone for other membrane associated proteins.To obtain insight into the role of αS in membrane remodeling we investigated the number of αS proteins associated with single small vesicles in a neuronal cell model. Using single-molecule microscopy and photo-bleaching approaches, we most frequently found 70 αS-GFPs per vesicle. Although this number is high enough to modulate physical membrane properties, it is also strikingly similar to the number of synaptobrevins, a putative interaction partner of αS, per vesicle. We therefore hypothesize a dual synergistic role for αS in membrane remodeling acting both as a modulator of the membrane’s physical properties and interaction partner for synaptobrevin.

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KW - number

KW - vesicles

KW - physiological function

KW - photo-bleaching

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ER -