The opto-locomotor reflex as a tool to measure sensitivity to moving random dot patterns in mice

L. A.M.H. Kirkels* (Corresponding Author), W. Zhang, M. N. Havenith, P. Tiesinga, J. Glennon, R. J.A. Van Wezel, J. Duijnhouwer

*Corresponding author for this work

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    Abstract

    We designed a method to quantify mice visual function by measuring reflexive opto-locomotor responses. Mice were placed on a Styrofoam ball at the center of a large dome on the inside of which we projected moving random dot patterns. Because we fixed the heads of the mice in space and the ball was floating on pressurized air, locomotion of the mice was translated to rotation of the ball, which we registered. Sudden onsets of rightward or leftward moving patterns caused the mice to reflexively change their running direction. We quantified the opto-locomotor responses to different pattern speeds, luminance contrasts, and dot sizes. We show that the method is fast and reliable and the magnitude of the reflex is stable within sessions. We conclude that this opto-locomotor reflex method is suitable to quantify visual function in mice.

    Original languageEnglish
    Article number7710
    JournalScientific reports
    Volume8
    Issue number1
    DOIs
    Publication statusPublished - 16 May 2018

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    Kirkels, L. A. M. H., Zhang, W., Havenith, M. N., Tiesinga, P., Glennon, J., Van Wezel, R. J. A., & Duijnhouwer, J. (2018). The opto-locomotor reflex as a tool to measure sensitivity to moving random dot patterns in mice. Scientific reports, 8(1), [7710]. https://doi.org/10.1038/s41598-018-25844-4