The synthesis and characterization of polypeptide-adriamycin conjugates and its complexes with adriamycin: Part I

W.A.R. Heeswijk, C.J.T. Hoes, T. Stoffer, M.J.D. Eenink, W. Potman, J. Feijen

Research output: Contribution to journalArticleAcademic

41 Citations (Scopus)
249 Downloads (Pure)

Abstract

Poly(α-l-glutamic acid) (PGA) was grafted with amino acid and oligopeptide spacers up to 5 amino acids with the use of N,N'-carbonyldiimidazole and 2,3-dihydro-1,2-benz-isothiazole-3-on-1, 1-dioxide (saccharin) as an additive, and these polypeptides were characterized. The antitumor antibiotic adriamycin was covalently coupled via an amide bond onto PGA and onto the grafted polymers with the use of N-ethoxycarbonyl-2-ethoxy-1, 2-dihydroquinoline (EEDQ); these conjugates were characterized. The conjugates containing Gly—Gly—l-Leu spacer arms did yield free adriamycin upon digestion with papain. Adriamycin gave fairly stable complexes with PGA—adriamycin and branched poly peptide—adriamycin conjugates; these complexes were characterized.
Original languageEnglish
Pages (from-to)301-315
JournalJournal of controlled release
Volume1
Issue number4
DOIs
Publication statusPublished - 1985

Fingerprint Dive into the research topics of 'The synthesis and characterization of polypeptide-adriamycin conjugates and its complexes with adriamycin: Part I'. Together they form a unique fingerprint.

Cite this