TY - JOUR
T1 - The use of 120-nm deep channels for liquid chromatographic separations
AU - Fekete, Veronika
AU - Clicq, David
AU - de Malsche, Wim
AU - Gardeniers, Johannes G.E.
AU - Desmet, Gert
N1 - 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques
PY - 2008
Y1 - 2008
N2 - The present study reports on the exploration of the separation speed limits of RPLC chromatography in open-tubular channels. Applying the shear-driven chromatography principle in a 120-nm deep open channel, and using an improved detection set-up, the separation of three coumarin dyes was detectable 8 mm downstream of the injection point. At this distance, separation efficiencies of N = 17,900–24,100 plates were obtained at a velocity of 10 mm s−1, corresponding to a plate generation velocity of 21,100 to 28,300 plates per second for the most and least retained component, respectively.
AB - The present study reports on the exploration of the separation speed limits of RPLC chromatography in open-tubular channels. Applying the shear-driven chromatography principle in a 120-nm deep open channel, and using an improved detection set-up, the separation of three coumarin dyes was detectable 8 mm downstream of the injection point. At this distance, separation efficiencies of N = 17,900–24,100 plates were obtained at a velocity of 10 mm s−1, corresponding to a plate generation velocity of 21,100 to 28,300 plates per second for the most and least retained component, respectively.
KW - METIS-247505
KW - IR-76301
U2 - 10.1016/j.chroma.2007.11.026
DO - 10.1016/j.chroma.2007.11.026
M3 - Article
SN - 0021-9673
VL - 1189
SP - 2
EP - 9
JO - Journal of chromatography A
JF - Journal of chromatography A
IS - 1-2
ER -