The use of self-assembled receptor layers in immunosensors

R.P.H. Kooyman; D.J. van den Heuvel; J.W. Drijfhout; G.W. Welling

doi:10.1016/0040-6090(94)90599-1

The use of self-assembled receptor layers in immunosensors

R.P.H. Kooyman, D.J. van den Heuvel, J.W. Drijfhout, G.W. Welling

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Abstract

We demonstrate that a self-assembled monolayer consisting of an alkylthiol coupled synthetic peptide adsorbed to a gold substrate results in a sensitive receptor surface for specific recognition of protein molecules. The affinity constant of this binding is comparable with that of an antibody-antigen reaction. Evidence is found that such a receptor surface is capable of reversible binding.

Original language	English
Pages (from-to)	913-916
Number of pages	4
Journal	Thin solid films
Volume	244
Issue number	1-2
DOIs	https://doi.org/10.1016/0040-6090(94)90599-1
Publication status	Published - 1994

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title = "The use of self-assembled receptor layers in immunosensors",

abstract = "We demonstrate that a self-assembled monolayer consisting of an alkylthiol coupled synthetic peptide adsorbed to a gold substrate results in a sensitive receptor surface for specific recognition of protein molecules. The affinity constant of this binding is comparable with that of an antibody-antigen reaction. Evidence is found that such a receptor surface is capable of reversible binding.",

author = "R.P.H. Kooyman and {van den Heuvel}, D.J. and J.W. Drijfhout and G.W. Welling",

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AU - Kooyman, R.P.H.

AU - van den Heuvel, D.J.

AU - Drijfhout, J.W.

AU - Welling, G.W.

PY - 1994

Y1 - 1994

N2 - We demonstrate that a self-assembled monolayer consisting of an alkylthiol coupled synthetic peptide adsorbed to a gold substrate results in a sensitive receptor surface for specific recognition of protein molecules. The affinity constant of this binding is comparable with that of an antibody-antigen reaction. Evidence is found that such a receptor surface is capable of reversible binding.

AB - We demonstrate that a self-assembled monolayer consisting of an alkylthiol coupled synthetic peptide adsorbed to a gold substrate results in a sensitive receptor surface for specific recognition of protein molecules. The affinity constant of this binding is comparable with that of an antibody-antigen reaction. Evidence is found that such a receptor surface is capable of reversible binding.

U2 - 10.1016/0040-6090(94)90599-1

DO - 10.1016/0040-6090(94)90599-1

M3 - Article

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VL - 244

SP - 913

EP - 916

JO - Thin solid films

JF - Thin solid films

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ER -

The use of self-assembled receptor layers in immunosensors

Abstract

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