Thermodynamically controlled synthesis of cefamandole

V.A. Nierstrasz, C.G.P.H. Schroen, R. Bosma, P.J. Kroon, H.H. Beeftink, A.E.M. Janssen, J. Tramper

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24 Citations (Scopus)


The direct enzymatic synthesis of the antibiotic cefamandole is presented as an alternative for the current processes based on either chemical synthesis or kinetic enzymatic synthesis. The influence of pH and temperature on the apparent equilibrium constant was measured; from these data, the maximum cefamandole concentration was predicted. In spite of a beneficial apparent equilibrium constant at pH 3.75, less product was formed as compared to the concentration at the optimum pH (4.25). This effect is caused by the low solubility of one of the substrates at pH 3.75.
Original languageEnglish
Pages (from-to)209-223
Number of pages15
JournalBiocatalysis and biotransformation
Issue number3
Publication statusPublished - 1999
Externally publishedYes


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