Thermodynamically controlled synthesis of cefamandole

V.A. Nierstrasz; C.G.P.H. Schroen; R. Bosma; P.J. Kroon; H.H. Beeftink; A.E.M. Janssen; J. Tramper

doi:10.3109/10242429909040115

Thermodynamically controlled synthesis of cefamandole

V.A. Nierstrasz, C.G.P.H. Schroen, R. Bosma, P.J. Kroon, H.H. Beeftink, A.E.M. Janssen, J. Tramper

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The direct enzymatic synthesis of the antibiotic cefamandole is presented as an alternative for the current processes based on either chemical synthesis or kinetic enzymatic synthesis. The influence of pH and temperature on the apparent equilibrium constant was measured; from these data, the maximum cefamandole concentration was predicted. In spite of a beneficial apparent equilibrium constant at pH 3.75, less product was formed as compared to the concentration at the optimum pH (4.25). This effect is caused by the low solubility of one of the substrates at pH 3.75.

Original language	English
Pages (from-to)	209-223
Number of pages	15
Journal	Biocatalysis and biotransformation
Volume	17
Issue number	3
DOIs	https://doi.org/10.3109/10242429909040115
Publication status	Published - 1999
Externally published	Yes

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abstract = "The direct enzymatic synthesis of the antibiotic cefamandole is presented as an alternative for the current processes based on either chemical synthesis or kinetic enzymatic synthesis. The influence of pH and temperature on the apparent equilibrium constant was measured; from these data, the maximum cefamandole concentration was predicted. In spite of a beneficial apparent equilibrium constant at pH 3.75, less product was formed as compared to the concentration at the optimum pH (4.25). This effect is caused by the low solubility of one of the substrates at pH 3.75.",

author = "V.A. Nierstrasz and C.G.P.H. Schroen and R. Bosma and P.J. Kroon and H.H. Beeftink and A.E.M. Janssen and J. Tramper",

year = "1999",

doi = "10.3109/10242429909040115",

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T1 - Thermodynamically controlled synthesis of cefamandole

AU - Nierstrasz, V.A.

AU - Schroen, C.G.P.H.

AU - Bosma, R.

AU - Kroon, P.J.

AU - Beeftink, H.H.

AU - Janssen, A.E.M.

AU - Tramper, J.

PY - 1999

Y1 - 1999

N2 - The direct enzymatic synthesis of the antibiotic cefamandole is presented as an alternative for the current processes based on either chemical synthesis or kinetic enzymatic synthesis. The influence of pH and temperature on the apparent equilibrium constant was measured; from these data, the maximum cefamandole concentration was predicted. In spite of a beneficial apparent equilibrium constant at pH 3.75, less product was formed as compared to the concentration at the optimum pH (4.25). This effect is caused by the low solubility of one of the substrates at pH 3.75.

AB - The direct enzymatic synthesis of the antibiotic cefamandole is presented as an alternative for the current processes based on either chemical synthesis or kinetic enzymatic synthesis. The influence of pH and temperature on the apparent equilibrium constant was measured; from these data, the maximum cefamandole concentration was predicted. In spite of a beneficial apparent equilibrium constant at pH 3.75, less product was formed as compared to the concentration at the optimum pH (4.25). This effect is caused by the low solubility of one of the substrates at pH 3.75.

U2 - 10.3109/10242429909040115

DO - 10.3109/10242429909040115

M3 - Article

SN - 1024-2422

VL - 17

SP - 209

EP - 223

JO - Biocatalysis and biotransformation

JF - Biocatalysis and biotransformation

IS - 3

ER -

Thermodynamically controlled synthesis of cefamandole

Abstract

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