Thermoresponsive giant biohybrid amphiphiles

Christine Lavigueur; Jordi Gonzalez Garcia; Linda Hendriks; Richard Hoogenboom; Jeroen Johannes Lambertus Maria Cornelissen; Roeland J.M. Nolte

doi:10.1039/C0PY00229A

Thermoresponsive giant biohybrid amphiphiles

Christine Lavigueur, Jordi Gonzalez Garcia, Linda Hendriks, Richard Hoogenboom, Jeroen Johannes Lambertus Maria Cornelissen, Roeland J.M. Nolte

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A series of random copolymers of various lengths was prepared by atom transfer radical polymerisation (ATRP) using two acrylate monomers with short pendant ethylene glycol side chains (ethylene glycol methyl ether acrylate, EGMEA, and methoxy ethoxy ethyl acrylate, MEEA). The end group was converted to an azide to enable bioconjugation through copper-catalysed azide–alkyne cycloaddition (CuAAC). All polymers were found to be thermoresponsive, with a cloud point between 25 and 35 °C depending on their molecular weight. They were conjugated to enhanced green fluorescent protein (EGFP) functionalised with a single alkyne moiety, as seen by fast performance liquid chromatography (FPLC) and gel electrophoresis (SDS-PAGE). The resulting biohybrid amphiphiles were thermoresponsive. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to study their self-assembly at elevated temperatures, and they were found to form spherical structures with a diameter of approximately 60 nm upon slow heating.

Original language	English
Pages (from-to)	333-340
Number of pages	7
Journal	Polymer chemistry
Volume	2
Issue number	2
DOIs	https://doi.org/10.1039/C0PY00229A
Publication status	Published - 2011

Keywords

IR-85342
METIS-283473

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10.1039/C0PY00229A

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title = "Thermoresponsive giant biohybrid amphiphiles",

abstract = "A series of random copolymers of various lengths was prepared by atom transfer radical polymerisation (ATRP) using two acrylate monomers with short pendant ethylene glycol side chains (ethylene glycol methyl ether acrylate, EGMEA, and methoxy ethoxy ethyl acrylate, MEEA). The end group was converted to an azide to enable bioconjugation through copper-catalysed azide–alkyne cycloaddition (CuAAC). All polymers were found to be thermoresponsive, with a cloud point between 25 and 35 °C depending on their molecular weight. They were conjugated to enhanced green fluorescent protein (EGFP) functionalised with a single alkyne moiety, as seen by fast performance liquid chromatography (FPLC) and gel electrophoresis (SDS-PAGE). The resulting biohybrid amphiphiles were thermoresponsive. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to study their self-assembly at elevated temperatures, and they were found to form spherical structures with a diameter of approximately 60 nm upon slow heating.",

keywords = "IR-85342, METIS-283473",

author = "Christine Lavigueur and {Gonzalez Garcia}, Jordi and Linda Hendriks and Richard Hoogenboom and Cornelissen, {Jeroen Johannes Lambertus Maria} and Nolte, {Roeland J.M.}",

year = "2011",

doi = "10.1039/C0PY00229A",

language = "English",

volume = "2",

pages = "333--340",

journal = "Polymer chemistry",

issn = "1759-9954",

publisher = "Royal Society of Chemistry",

number = "2",

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TY - JOUR

T1 - Thermoresponsive giant biohybrid amphiphiles

AU - Lavigueur, Christine

AU - Gonzalez Garcia, Jordi

AU - Hendriks, Linda

AU - Hoogenboom, Richard

AU - Cornelissen, Jeroen Johannes Lambertus Maria

AU - Nolte, Roeland J.M.

PY - 2011

Y1 - 2011

N2 - A series of random copolymers of various lengths was prepared by atom transfer radical polymerisation (ATRP) using two acrylate monomers with short pendant ethylene glycol side chains (ethylene glycol methyl ether acrylate, EGMEA, and methoxy ethoxy ethyl acrylate, MEEA). The end group was converted to an azide to enable bioconjugation through copper-catalysed azide–alkyne cycloaddition (CuAAC). All polymers were found to be thermoresponsive, with a cloud point between 25 and 35 °C depending on their molecular weight. They were conjugated to enhanced green fluorescent protein (EGFP) functionalised with a single alkyne moiety, as seen by fast performance liquid chromatography (FPLC) and gel electrophoresis (SDS-PAGE). The resulting biohybrid amphiphiles were thermoresponsive. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to study their self-assembly at elevated temperatures, and they were found to form spherical structures with a diameter of approximately 60 nm upon slow heating.

AB - A series of random copolymers of various lengths was prepared by atom transfer radical polymerisation (ATRP) using two acrylate monomers with short pendant ethylene glycol side chains (ethylene glycol methyl ether acrylate, EGMEA, and methoxy ethoxy ethyl acrylate, MEEA). The end group was converted to an azide to enable bioconjugation through copper-catalysed azide–alkyne cycloaddition (CuAAC). All polymers were found to be thermoresponsive, with a cloud point between 25 and 35 °C depending on their molecular weight. They were conjugated to enhanced green fluorescent protein (EGFP) functionalised with a single alkyne moiety, as seen by fast performance liquid chromatography (FPLC) and gel electrophoresis (SDS-PAGE). The resulting biohybrid amphiphiles were thermoresponsive. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to study their self-assembly at elevated temperatures, and they were found to form spherical structures with a diameter of approximately 60 nm upon slow heating.

KW - IR-85342

KW - METIS-283473

U2 - 10.1039/C0PY00229A

DO - 10.1039/C0PY00229A

M3 - Article

VL - 2

SP - 333

EP - 340

JO - Polymer chemistry

JF - Polymer chemistry

SN - 1759-9954

IS - 2

ER -

Thermoresponsive giant biohybrid amphiphiles

Abstract

Keywords

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