Thermosensitive hydrogel-containing polymersomes for controlled drug delivery

Jung Seok Lee, W. Zhou, Fenghua Meng, D. Zhang, Cornelis Otto, Jan Feijen

Research output: Contribution to journalArticleAcademicpeer-review

61 Citations (Scopus)

Abstract

PNIPAAm-containing polymersomes (N/Ps) were prepared by injecting a solution of poly(ethylene glycol)-b-poly(D,L-lactide) (mPEG-PDLLA) and poly(N-isopropylacrylamide) (PNIPAAm) in THF into water to incorporate PNIPAAm into polymersomes (Ps). At 37 °C, hydrogel-containing Ps (Hs, hydrosomes) with an average diameter of 127 nm as measured with dynamic light scattering (DLS) were obtained which may be used as potential novel carriers for anticancer drugs and proteins. Dual-labeled N/Ps (FITC-N/RB-Ps) were prepared analogously using rhodamine B tagged mPEG-PDLLA (mPEG-PDLLA-RB) and fluorescein isothiocyanate labeled PNIPAAm (FITC-N). The co-localization of RB labeled Ps (RB-Ps) and FITC-N in RB-Ps was shown by dual fluorescence CLSM. Fluorescence correlation spectroscopy (FCS) and fluorescence anisotropy (FA) measurements with these systems gave further evidence for the colocalization of PNIPAAm and Ps. Micron-sized giant Ps with a diameter of 5–10 μm containing FITC-N were prepared using CHCl3 as the organic phase. The presence of FITC-N in these giant Ps as well as the phase separation of the internal FITC-N solution above the lower critical solution temperature (LCST) was also shown by CLSM. The release of fluorescein isothiocyanate tagged dextran (FD, FITC-dextran, Mw 4000 g/mol) from Hs revealed that in the presence of the hydrogel at 37 °C a more sustained release of FD (up to 30 days) with a low initial burst effect was obtained as compared to the release from bare Ps.
Original languageEnglish
Pages (from-to)400-408
Number of pages8
JournalJournal of controlled release
Volume146
Issue number3
DOIs
Publication statusPublished - 2010

Fingerprint

Hydrogel
Fluorescein
Pharmaceutical Preparations
rhodamine B
poly-N-isopropylacrylamide
Drug Carriers
Fluorescence Polarization
Ethylene Glycol
Fluorescein-5-isothiocyanate
Fluorescence Spectrometry
isothiocyanic acid
Fluorescence
Temperature
Water

Keywords

  • METIS-274971
  • Controlled drug delivery
  • Biodegradable polymersomes
  • IR-76588
  • Thermosensitive hydrogel
  • Poly(ethylene glycol)-b-poly(D
  • Poly(N-isopropylacrylamide)
  • L-lactide

Cite this

Lee, Jung Seok ; Zhou, W. ; Meng, Fenghua ; Zhang, D. ; Otto, Cornelis ; Feijen, Jan. / Thermosensitive hydrogel-containing polymersomes for controlled drug delivery. In: Journal of controlled release. 2010 ; Vol. 146, No. 3. pp. 400-408.
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abstract = "PNIPAAm-containing polymersomes (N/Ps) were prepared by injecting a solution of poly(ethylene glycol)-b-poly(D,L-lactide) (mPEG-PDLLA) and poly(N-isopropylacrylamide) (PNIPAAm) in THF into water to incorporate PNIPAAm into polymersomes (Ps). At 37 °C, hydrogel-containing Ps (Hs, hydrosomes) with an average diameter of 127 nm as measured with dynamic light scattering (DLS) were obtained which may be used as potential novel carriers for anticancer drugs and proteins. Dual-labeled N/Ps (FITC-N/RB-Ps) were prepared analogously using rhodamine B tagged mPEG-PDLLA (mPEG-PDLLA-RB) and fluorescein isothiocyanate labeled PNIPAAm (FITC-N). The co-localization of RB labeled Ps (RB-Ps) and FITC-N in RB-Ps was shown by dual fluorescence CLSM. Fluorescence correlation spectroscopy (FCS) and fluorescence anisotropy (FA) measurements with these systems gave further evidence for the colocalization of PNIPAAm and Ps. Micron-sized giant Ps with a diameter of 5–10 μm containing FITC-N were prepared using CHCl3 as the organic phase. The presence of FITC-N in these giant Ps as well as the phase separation of the internal FITC-N solution above the lower critical solution temperature (LCST) was also shown by CLSM. The release of fluorescein isothiocyanate tagged dextran (FD, FITC-dextran, Mw 4000 g/mol) from Hs revealed that in the presence of the hydrogel at 37 °C a more sustained release of FD (up to 30 days) with a low initial burst effect was obtained as compared to the release from bare Ps.",
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Thermosensitive hydrogel-containing polymersomes for controlled drug delivery. / Lee, Jung Seok; Zhou, W.; Meng, Fenghua; Zhang, D.; Otto, Cornelis; Feijen, Jan.

In: Journal of controlled release, Vol. 146, No. 3, 2010, p. 400-408.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Thermosensitive hydrogel-containing polymersomes for controlled drug delivery

AU - Lee, Jung Seok

AU - Zhou, W.

AU - Meng, Fenghua

AU - Zhang, D.

AU - Otto, Cornelis

AU - Feijen, Jan

PY - 2010

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N2 - PNIPAAm-containing polymersomes (N/Ps) were prepared by injecting a solution of poly(ethylene glycol)-b-poly(D,L-lactide) (mPEG-PDLLA) and poly(N-isopropylacrylamide) (PNIPAAm) in THF into water to incorporate PNIPAAm into polymersomes (Ps). At 37 °C, hydrogel-containing Ps (Hs, hydrosomes) with an average diameter of 127 nm as measured with dynamic light scattering (DLS) were obtained which may be used as potential novel carriers for anticancer drugs and proteins. Dual-labeled N/Ps (FITC-N/RB-Ps) were prepared analogously using rhodamine B tagged mPEG-PDLLA (mPEG-PDLLA-RB) and fluorescein isothiocyanate labeled PNIPAAm (FITC-N). The co-localization of RB labeled Ps (RB-Ps) and FITC-N in RB-Ps was shown by dual fluorescence CLSM. Fluorescence correlation spectroscopy (FCS) and fluorescence anisotropy (FA) measurements with these systems gave further evidence for the colocalization of PNIPAAm and Ps. Micron-sized giant Ps with a diameter of 5–10 μm containing FITC-N were prepared using CHCl3 as the organic phase. The presence of FITC-N in these giant Ps as well as the phase separation of the internal FITC-N solution above the lower critical solution temperature (LCST) was also shown by CLSM. The release of fluorescein isothiocyanate tagged dextran (FD, FITC-dextran, Mw 4000 g/mol) from Hs revealed that in the presence of the hydrogel at 37 °C a more sustained release of FD (up to 30 days) with a low initial burst effect was obtained as compared to the release from bare Ps.

AB - PNIPAAm-containing polymersomes (N/Ps) were prepared by injecting a solution of poly(ethylene glycol)-b-poly(D,L-lactide) (mPEG-PDLLA) and poly(N-isopropylacrylamide) (PNIPAAm) in THF into water to incorporate PNIPAAm into polymersomes (Ps). At 37 °C, hydrogel-containing Ps (Hs, hydrosomes) with an average diameter of 127 nm as measured with dynamic light scattering (DLS) were obtained which may be used as potential novel carriers for anticancer drugs and proteins. Dual-labeled N/Ps (FITC-N/RB-Ps) were prepared analogously using rhodamine B tagged mPEG-PDLLA (mPEG-PDLLA-RB) and fluorescein isothiocyanate labeled PNIPAAm (FITC-N). The co-localization of RB labeled Ps (RB-Ps) and FITC-N in RB-Ps was shown by dual fluorescence CLSM. Fluorescence correlation spectroscopy (FCS) and fluorescence anisotropy (FA) measurements with these systems gave further evidence for the colocalization of PNIPAAm and Ps. Micron-sized giant Ps with a diameter of 5–10 μm containing FITC-N were prepared using CHCl3 as the organic phase. The presence of FITC-N in these giant Ps as well as the phase separation of the internal FITC-N solution above the lower critical solution temperature (LCST) was also shown by CLSM. The release of fluorescein isothiocyanate tagged dextran (FD, FITC-dextran, Mw 4000 g/mol) from Hs revealed that in the presence of the hydrogel at 37 °C a more sustained release of FD (up to 30 days) with a low initial burst effect was obtained as compared to the release from bare Ps.

KW - METIS-274971

KW - Controlled drug delivery

KW - Biodegradable polymersomes

KW - IR-76588

KW - Thermosensitive hydrogel

KW - Poly(ethylene glycol)-b-poly(D

KW - Poly(N-isopropylacrylamide)

KW - L-lactide

U2 - 10.1016/j.corel.2010.06.002

DO - 10.1016/j.corel.2010.06.002

M3 - Article

VL - 146

SP - 400

EP - 408

JO - Journal of controlled release

JF - Journal of controlled release

SN - 0168-3659

IS - 3

ER -