Thermosensitive hydrogels for the encapsulation of primary and human derived neuronal cells

Donatella Di Lisa; Elena Dellacasa; Lorenzo Muzzi; Alberto Lagazzo; Monica Frega; Sergio Martinoia; Laura Pastorino

Thermosensitive hydrogels for the encapsulation of primary and human derived neuronal cells

Donatella Di Lisa, Elena Dellacasa, Lorenzo Muzzi, Alberto Lagazzo, Monica Frega, Sergio Martinoia, Laura Pastorino

Clinical Neurophysiology

Research output: Contribution to journal › Conference article › Academic › peer-review

Abstract

Thermosensitive chitosan-based hydrogel was developed to encapsulate neuronal cells and to support neuronal network development. In particular, the goal of this study was to demonstrate the versatility of this hydrogel to be used as an artificial matrix for 3D neuronal networks in vitro studies and as an injectable-hydrogel for the in-vivo applications or innovative ink for 3D bioprinter. To this aim, chitosan thermogels were fabricated and characterized by different techniques. The scaffold was used to encapsulate primary rat neurons and human-induced neurons cultures to carry out morphological characterization by immunofluorescence techniques.

Original language	English
Pages (from-to)	438-441
Number of pages	4
Journal	Convegno Nazionale di Bioingegneria
Publication status	Published - 2020
Event	7th National Congress of Bioengineering, GNB 2020 - Trieste, Italy Duration: 9 Jun 2020 → 11 Jun 2020 Conference number: 7

Keywords

3D neuronal networks
Chitosan
h-iPSCs
injectability
thermogel
NLA

Cite this

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title = "Thermosensitive hydrogels for the encapsulation of primary and human derived neuronal cells",

abstract = "Thermosensitive chitosan-based hydrogel was developed to encapsulate neuronal cells and to support neuronal network development. In particular, the goal of this study was to demonstrate the versatility of this hydrogel to be used as an artificial matrix for 3D neuronal networks in vitro studies and as an injectable-hydrogel for the in-vivo applications or innovative ink for 3D bioprinter. To this aim, chitosan thermogels were fabricated and characterized by different techniques. The scaffold was used to encapsulate primary rat neurons and human-induced neurons cultures to carry out morphological characterization by immunofluorescence techniques.",

keywords = "3D neuronal networks, Chitosan, h-iPSCs, injectability, thermogel, NLA",

author = "{Di Lisa}, Donatella and Elena Dellacasa and Lorenzo Muzzi and Alberto Lagazzo and Monica Frega and Sergio Martinoia and Laura Pastorino",

note = "Funding Information: The D. Di Lisa research activity is co-financed by Programma Operativo Por FSE Regione Liguria 2014-2020, n. RLOF18ASSRIC”. Publisher Copyright: {\textcopyright} 2020 Convegno Nazionale di Bioingegneria. All rights reserved.; 7th National Congress of Bioengineering, GNB 2020, GNB 2020 ; Conference date: 09-06-2020 Through 11-06-2020",

year = "2020",

language = "English",

pages = "438--441",

journal = "Convegno Nazionale di Bioingegneria",

issn = "2724-2129",

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}

TY - JOUR

T1 - Thermosensitive hydrogels for the encapsulation of primary and human derived neuronal cells

AU - Di Lisa, Donatella

AU - Dellacasa, Elena

AU - Muzzi, Lorenzo

AU - Lagazzo, Alberto

AU - Frega, Monica

AU - Martinoia, Sergio

AU - Pastorino, Laura

N1 - Conference code: 7

PY - 2020

Y1 - 2020

N2 - Thermosensitive chitosan-based hydrogel was developed to encapsulate neuronal cells and to support neuronal network development. In particular, the goal of this study was to demonstrate the versatility of this hydrogel to be used as an artificial matrix for 3D neuronal networks in vitro studies and as an injectable-hydrogel for the in-vivo applications or innovative ink for 3D bioprinter. To this aim, chitosan thermogels were fabricated and characterized by different techniques. The scaffold was used to encapsulate primary rat neurons and human-induced neurons cultures to carry out morphological characterization by immunofluorescence techniques.

AB - Thermosensitive chitosan-based hydrogel was developed to encapsulate neuronal cells and to support neuronal network development. In particular, the goal of this study was to demonstrate the versatility of this hydrogel to be used as an artificial matrix for 3D neuronal networks in vitro studies and as an injectable-hydrogel for the in-vivo applications or innovative ink for 3D bioprinter. To this aim, chitosan thermogels were fabricated and characterized by different techniques. The scaffold was used to encapsulate primary rat neurons and human-induced neurons cultures to carry out morphological characterization by immunofluorescence techniques.

KW - 3D neuronal networks

KW - Chitosan

KW - h-iPSCs

KW - injectability

KW - thermogel

KW - NLA

UR - http://www.scopus.com/inward/record.url?scp=85149383872&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:85149383872

SN - 2724-2129

SP - 438

EP - 441

JO - Convegno Nazionale di Bioingegneria

JF - Convegno Nazionale di Bioingegneria

T2 - 7th National Congress of Bioengineering, GNB 2020

Y2 - 9 June 2020 through 11 June 2020

ER -

Thermosensitive hydrogels for the encapsulation of primary and human derived neuronal cells

Abstract

Keywords

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