Third-generation amperometric biosensor for glucose: Polypyrrole deposited within a matrix of uniform latex particles as mediator

Cornelis G.J. Koopal, Martinus C. Feiters, Roeland J.M. Nolte*, Barteld de Ruiter, Richard B.M. Schasfoort

*Corresponding author for this work

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Abstract

Uniform latex particles and agarose gels are utilized to create porous membranes in which polypyrrole is electrochemically synthesized. Within the pores of these modified membranes glucose oxidase can be adsorbed irreversibly while its catalytic activity is retained. A glucose sensor which has a considerable lifetime under continuous use is constructed from the enzyme membrane. With this sensor glucose can be measured amperometrically in the concentration range 1-60 mM. The sensor response is independent of oxygen concentration.

Original languageEnglish
Pages (from-to)159-175
Number of pages17
JournalBioelectrochemistry and bioenergetics
Volume29
Issue number2
DOIs
Publication statusPublished - 1 Jan 1992
Externally publishedYes

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