Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex

Irina Stoyanova, Remy Wiertz, Wim Rutten

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    Abstract

    Ghrelin is a hormone, initially described as a gastric peptide stimulating appetite and growth hormone secretion, which also has an important role in the regulation of many other processes, including higher brain functions. Ghrelin has been described in situ in different parts of the brain, but so far there has been no data about its expression in cell cultures. Therefore, we aimed in this study to investigate the levels of ghrelin in dissociated cortical neurons at various times in culture. We applied the ABC immunocytochemical method for the detection of ghrelin in one-day-, one-week-, and two-week-old cultures. Our results clearly show that at the early stages after plating the cultures 86.2% (± 8.93) of the neurons are ghrelin-positive and their number decreases during the culturing period. As ghrelin is present in the majority of cultured newborn neurons, when the neuronal differentiation and network formation take place, it may also influence the early synaptic formation and cell-to-cell interactions, which are both very important for network functions like learning and memory.
    Original languageUndefined
    Article number10.1016/j.regpep.2009.06.008
    Pages (from-to)86-90
    Number of pages5
    JournalRegulatory Peptides
    Volume158
    Issue number1-3
    DOIs
    Publication statusPublished - 27 Nov 2009

    Keywords

    • dissociated neuronal cultures
    • EWI-15954
    • Ghrelin expression
    • METIS-266485
    • IR-70130
    • BSS-Neurotechnology and cellular engineering

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