Time domain algorithm for whole field laser Doppler perfusion imaging

Matthijs Draijer, Erwin Hondebrink, Ton van Leeuwen, Wiendelt Steenbergen

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Recently, various groups have developed wide field laser Doppler perfusion imaging systems based on high speed camera's. The limiting factor for the frame rate and measurement duration in whole field laser Doppler perfusion imaging is the speed of transfer and analysis of data. We present an algorithm for calculating perfusion estimations with much lower demands for data storage and computational effort than the conventional FFT-based method. Our algorithm works in the time domain and estimates perfusion through simple time differentiations and multiplications of speckle image values. The algorithm is partly based on mathematical reasoning, and partly on a hypothesis that cannot be proven with rigorous mathematics. We will compare our algorithm with the frequency-domain counterpart for phantom studies involving static and dynamic media, and in vivo experiments on human skin. It is found that both algorithms, applied on the same dataset, approximately give the same perfusion estimations. The random differences are similar to the random variations found in tissue perfusion. Systematic differences between the algorithms smaller than 15% are found. The algorithm is currently twice as fast as the FFT-counterpart. Another advantage is that our algorithm can be included in a moving average scheme, where a new perfusion value can be determined based on the previous value and a small number of new raw speckle images
Original languageUndefined
Title of host publicationOptical Diagnostics and Sensing IX
EditorsGerard L. Cote
Place of PublicationBellingham
ISBN (Print)9780819474322
Publication statusPublished - 24 Jan 2009
EventSPIE International Symposium on Biomedical Optics, BIOS 2009 - San Jose, United States
Duration: 24 Jan 200929 Jan 2009

Publication series

NameProceedings of SPIE
ISSN (Print)1605-7422


ConferenceSPIE International Symposium on Biomedical Optics, BIOS 2009
Country/TerritoryUnited States
CitySan Jose


  • Signal processing
  • perfusion imaging
  • Laser-Doppler
  • METIS-258862
  • Microcirculation
  • IR-77778

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