Time-resolved absolute radius estimation of vibrating contrast microbubbles using an acoustical camera

Sander Spiekhout*, Jason Voorneveld, Benjamin van Elburg, Guillaume Renaud, Tim Segers, Guillaume P. R. Lajoinie, Michel Versluis, Martin D. Verweij, Nico de Jong, Johannes G. Bosch

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Ultrasound (US) contrast agents consist of microbubbles ranging from 1 to 10 μm in size. The acoustical response of individual microbubbles can be studied with high-frame-rate optics or an “acoustical camera” (AC). The AC measures the relative microbubble oscillation while the optical camera measures the absolute oscillation. In this article, the capabilities of the AC are extended to measure the absolute oscillations. In the AC setup, microbubbles are insonified with a high- (25 MHz) and low-frequency US wave (1–2.5 MHz). Other than the amplitude modulation (AM) from the relative size change of the microbubble (employed in Renaud, Bosch, van der Steen, and de Jong (2012a). “An ‘acoustical camera’ for in vitro characterization of contrast agent microbubble vibrations,” Appl. Phys. Lett. 100(10), 101911, the high-frequency response from individual vibrating microbubbles contains a phase modulation (PM) from the microbubble wall displacement, which is the extension described here. The ratio of PM and AM is used to determine the absolute radius, R0. To test this sizing, the size distributions of two monodisperse microbubble populations (𝑅0=R0= 2.1 and 3.5 μm) acquired with the AC were matched to the distribution acquired with a Coulter counter. As a result of measuring the absolute size of the microbubbles, this “extended AC” can capture the full radial dynamics of single freely floating microbubbles with a throughput of hundreds of microbubbles per hour.
Original languageEnglish
Article number3993
Pages (from-to)3993-4003
Number of pages11
JournalThe Journal of the Acoustical Society of America
Volume151
Issue number6
Early online date14 Jun 2022
DOIs
Publication statusPublished - Jun 2022

Keywords

  • 22/2 OA procedure

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