Abstract
To analyze the molecular content of single cells, cell lysis is typically required, yielding a snapshot of cell behavior only. To follow complex molecular profiles over time, subcellular sampling methods potentially can be used, but to date these methods involve laborious offline analysis. Here we report a "tip chip" device for continuous online coupling of subcellular sampling and on-chip sample analysis. The device contains a hollow, sharp, out-of-plane tip connected to a microfluidic channel structure. After positioning a cell onto the tip using a fluidic force microscope, intracellular molecules diffuse into the microchannel and are directly analyzed using isotachophoresis.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | The Chemical and Biological Microsystems Society |
| Pages | 541-542 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| Publication status | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 Conference number: 20 http://www.microtas2016.org/ |
Conference
| Conference | 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 |
|---|---|
| Abbreviated title | MicroTAS 2016 |
| Country/Territory | Ireland |
| City | Dublin |
| Period | 9/10/16 → 13/10/16 |
| Internet address |
Keywords
- Fluidic force microscopy
- Isotachophoresis
- Single-cell analysis
- Subcellular sampling
