Tip chip: Subcellular sampling from single cancer cells

Jos Quist*, Edin Sarajlic, Stanley C.S. Lai, Serge G. Lemay

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

To analyze the molecular content of single cells, cell lysis is typically required, yielding a snapshot of cell behavior only. To follow complex molecular profiles over time, subcellular sampling methods potentially can be used, but to date these methods involve laborious offline analysis. Here we report a "tip chip" device for continuous online coupling of subcellular sampling and on-chip sample analysis. The device contains a hollow, sharp, out-of-plane tip connected to a microfluidic channel structure. After positioning a cell onto the tip using a fluidic force microscope, intracellular molecules diffuse into the microchannel and are directly analyzed using isotachophoresis.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherThe Chemical and Biological Microsystems Society
Pages541-542
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016
Conference number: 20
http://www.microtas2016.org/

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016
Abbreviated titleMicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16
Internet address

Keywords

  • Fluidic force microscopy
  • Isotachophoresis
  • Single-cell analysis
  • Subcellular sampling

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