Top-and-side dual-view microfluidic device with embedded prism

Yuto Kazama; Edwin T. Carlen; Albert van den Berg; Akihide Hibara

doi:10.1016/j.snb.2017.04.013

Top-and-side dual-view microfluidic device with embedded prism

Yuto Kazama, Edwin T. Carlen, Albert van den Berg, Akihide Hibara^*

^*Corresponding author for this work

Biomedical and Environmental Sensorsystems

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

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Abstract

A polydimethylsiloxane microfluidic device enabling dual-view visualization is proposed and demonstrated. A prism with a 2 mm square base was embedded beside a 300 μm-wide microchannel. In addition to ordinary visualization from the top of the device, the microchannel could be viewed from the side, and its optical path was reflected to the top by the prism. The top and side dual visualization in a single field of view was then realized with a single objective lens. The shifts in the focal point in the top and side directions were modeled, and a compensation method utilizing a flat sheet was used. After simultaneous bright-field and dark-field visualization was attained, dual-view fluorescence imaging of the fluorescent solution and cells was realized.

Original language	English
Pages (from-to)	753-760
Number of pages	8
Journal	Sensors and Actuators B: Chemical
Volume	248
DOIs	https://doi.org/10.1016/j.snb.2017.04.013
Publication status	Published - 2017

Keywords

Dark-field view
Dual view
Fluorescence
Microfluidic channel
PDMS
2023 OA procedure

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10.1016/j.snb.2017.04.013

ArticleFinal published version, 2.52 MBLicence: Taverne

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title = "Top-and-side dual-view microfluidic device with embedded prism",

abstract = "A polydimethylsiloxane microfluidic device enabling dual-view visualization is proposed and demonstrated. A prism with a 2 mm square base was embedded beside a 300 μm-wide microchannel. In addition to ordinary visualization from the top of the device, the microchannel could be viewed from the side, and its optical path was reflected to the top by the prism. The top and side dual visualization in a single field of view was then realized with a single objective lens. The shifts in the focal point in the top and side directions were modeled, and a compensation method utilizing a flat sheet was used. After simultaneous bright-field and dark-field visualization was attained, dual-view fluorescence imaging of the fluorescent solution and cells was realized.",

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author = "Yuto Kazama and Carlen, {Edwin T.} and {van den Berg}, Albert and Akihide Hibara",

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AU - Kazama, Yuto

AU - Carlen, Edwin T.

AU - van den Berg, Albert

AU - Hibara, Akihide

PY - 2017

Y1 - 2017

N2 - A polydimethylsiloxane microfluidic device enabling dual-view visualization is proposed and demonstrated. A prism with a 2 mm square base was embedded beside a 300 μm-wide microchannel. In addition to ordinary visualization from the top of the device, the microchannel could be viewed from the side, and its optical path was reflected to the top by the prism. The top and side dual visualization in a single field of view was then realized with a single objective lens. The shifts in the focal point in the top and side directions were modeled, and a compensation method utilizing a flat sheet was used. After simultaneous bright-field and dark-field visualization was attained, dual-view fluorescence imaging of the fluorescent solution and cells was realized.

AB - A polydimethylsiloxane microfluidic device enabling dual-view visualization is proposed and demonstrated. A prism with a 2 mm square base was embedded beside a 300 μm-wide microchannel. In addition to ordinary visualization from the top of the device, the microchannel could be viewed from the side, and its optical path was reflected to the top by the prism. The top and side dual visualization in a single field of view was then realized with a single objective lens. The shifts in the focal point in the top and side directions were modeled, and a compensation method utilizing a flat sheet was used. After simultaneous bright-field and dark-field visualization was attained, dual-view fluorescence imaging of the fluorescent solution and cells was realized.

KW - Dark-field view

KW - Dual view

KW - Fluorescence

KW - Microfluidic channel

KW - PDMS

KW - 2023 OA procedure

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M3 - Article

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EP - 760

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Top-and-side dual-view microfluidic device with embedded prism

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Keywords

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