Top-and-side dual-view microfluidic device with embedded prism

Yuto Kazama, Edwin T. Carlen, Albert van den Berg, Akihide Hibara*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)
1 Downloads (Pure)


A polydimethylsiloxane microfluidic device enabling dual-view visualization is proposed and demonstrated. A prism with a 2 mm square base was embedded beside a 300 μm-wide microchannel. In addition to ordinary visualization from the top of the device, the microchannel could be viewed from the side, and its optical path was reflected to the top by the prism. The top and side dual visualization in a single field of view was then realized with a single objective lens. The shifts in the focal point in the top and side directions were modeled, and a compensation method utilizing a flat sheet was used. After simultaneous bright-field and dark-field visualization was attained, dual-view fluorescence imaging of the fluorescent solution and cells was realized.

Original languageEnglish
Pages (from-to)753-760
Number of pages8
JournalSensors and actuators. B: Chemical
Publication statusPublished - 2017


  • Dark-field view
  • Dual view
  • Fluorescence
  • Microfluidic channel
  • PDMS

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