TY - JOUR
T1 - Toward a real liquid biopsy in metastatic breast and prostate cancer
T2 - Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap)
AU - Andree, Kiki C.
AU - Mentink, Anouk
AU - Zeune, Leonie L.
AU - Terstappen, Leon W.M.M.
AU - Stoecklein, Nikolas H.
AU - Neves, Rui P.
AU - Driemel, Christiane
AU - Lampignano, Rita
AU - Yang, Liwen
AU - Neubauer, Hans
AU - Fehm, Tanja
AU - Fischer, Johannes C.
AU - Rossi, Elisabetta
AU - Manicone, Mariangela
AU - Basso, Umberto
AU - Marson, Piero
AU - Zamarchi, Rita
AU - Loriot, Yohann
AU - Lapierre, Valerie
AU - Faugeroux, Vincent
AU - Oulhen, Marianne
AU - Farace, Françoise
AU - Fowler, Gemma
AU - Sousa Fontes, Mariane
AU - Ebbs, Berni
AU - Lambros, Maryou
AU - Crespo, Mateus
AU - Flohr, Penny
AU - de Bono, Johann S.
N1 - Wiley deal
PY - 2018/11/15
Y1 - 2018/11/15
N2 - Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL of blood is too small to reliably determine tumor heterogeneity and to be representative as a “liquid biopsy”. In the EU FP7 program CTCTrap, we aimed to validate and optimize the recently introduced Diagnostic LeukApheresis (DLA) to screen liters of blood. Here we present the results obtained from 34 metastatic cancer patients subjected to DLA in the participating institutions. About 7.5 mL blood processed with CellSearch® was used as “gold standard” reference. DLAs were obtained from 22 metastatic prostate and 12 metastatic breast cancer patients at four different institutions without any noticeable side effects. DLA samples were prepared and processed with different analysis techniques. Processing DLA using CellSearch resulted in a 0–32 fold increase in CTC yield compared to processing 7.5 mL blood. Filtration of DLA through 5 μm pores microsieves was accompanied by large CTC losses. Leukocyte depletion of 18 mL followed by CellSearch yielded an increase of the number of CTC but a relative decrease in yield (37%) versus CellSearch DLA. In four out of seven patients with 0 CTC detected in 7.5 mL of blood, CTC were detected in DLA (range 1–4 CTC). The CTC obtained through DLA enables molecular characterization of the tumor. CTC enrichment technologies however still need to be improved to isolate all the CTC present in the DLA.
AB - Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL of blood is too small to reliably determine tumor heterogeneity and to be representative as a “liquid biopsy”. In the EU FP7 program CTCTrap, we aimed to validate and optimize the recently introduced Diagnostic LeukApheresis (DLA) to screen liters of blood. Here we present the results obtained from 34 metastatic cancer patients subjected to DLA in the participating institutions. About 7.5 mL blood processed with CellSearch® was used as “gold standard” reference. DLAs were obtained from 22 metastatic prostate and 12 metastatic breast cancer patients at four different institutions without any noticeable side effects. DLA samples were prepared and processed with different analysis techniques. Processing DLA using CellSearch resulted in a 0–32 fold increase in CTC yield compared to processing 7.5 mL blood. Filtration of DLA through 5 μm pores microsieves was accompanied by large CTC losses. Leukocyte depletion of 18 mL followed by CellSearch yielded an increase of the number of CTC but a relative decrease in yield (37%) versus CellSearch DLA. In four out of seven patients with 0 CTC detected in 7.5 mL of blood, CTC were detected in DLA (range 1–4 CTC). The CTC obtained through DLA enables molecular characterization of the tumor. CTC enrichment technologies however still need to be improved to isolate all the CTC present in the DLA.
KW - UT-Hybrid-D
KW - Circulating tumor cells (CTC)
KW - Liquid biopsy
KW - Filtration
KW - CellSearch
KW - Diagnostic leukapheresis
KW - filtration
KW - circulating tumor cells
KW - diagnostic leukapheresis
KW - liquid biopsy
UR - http://www.scopus.com/inward/record.url?scp=85053496687&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/toward-real-liquid-biopsy-metastatic-breast-prostate-cancer-diagnostic-leukapheresis-increases-ctc-y
U2 - 10.1002/ijc.31752
DO - 10.1002/ijc.31752
M3 - Article
AN - SCOPUS:85053496687
SN - 0020-7136
VL - 143
SP - 2584
EP - 2591
JO - International journal of cancer
JF - International journal of cancer
IS - 10
ER -